生物多样性 ›› 2018, Vol. 26 ›› Issue (12): 1289-1295.DOI: 10.17520/biods.2018121

• 研究报告 • 上一篇    下一篇

西江中下游鳤的遗传多样性与种群动态历史

杨计平1, 李策1,2, 陈蔚涛1, 李跃飞1, 李新辉1,*()   

  1. 1 中国水产科学研究院珠江水产研究所, 广州 510380
    2 上海海洋大学海洋科学学院, 上海 201306
  • 收稿日期:2018-04-16 接受日期:2018-04-16 出版日期:2018-12-20 发布日期:2019-02-11
  • 通讯作者: 李新辉
  • 作者简介:# 共同第一作者
  • 基金资助:
    中国水产科学研究院基本科研业务费(2016HY-ZC0503)

Genetic diversity and population demographic history of Ochetobius elongatus in the middle and lower reaches of the Xijiang River

Jiping Yang1, Ce Li1,2, Weitao Chen1, Yuefei Li1, Xinhui Li1,*()   

  1. 1 Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380
    2 College of Marine Sciences, Shanghai Ocean University, Shanghai 201306
  • Received:2018-04-16 Accepted:2018-04-16 Online:2018-12-20 Published:2019-02-11
  • Contact: Li Xinhui
  • About author:# 同等贡献作者 Contributed equally to this work

摘要:

鳤(Ochetobius elongatus)曾经是我国许多河流的重要经济鱼类, 然而由于环境污染和人为干扰等原因, 鳤的资源量萎缩严重, 种群处于极度濒危的境地。目前, 常规采样方法获得鳤样本的难度较大, 致使相关研究难以开展。本研究通过仔鱼采集和成鱼采集的手段, 测定两个线粒体基因和两个核基因, 对西江中下游鳤的遗传多样性和种群动态历史进行研究。结果显示, 西江中下游鳤的遗传多样性处于较低水平并且出现了衰退的迹象, 可能经历了遗传瓶颈效应。此外, 种群动态历史分析发现, 西江中下游鳤在后更新世期间(0.06和0.13百万年前)经历了种群扩张, 刚好处于中更新世(0.78-0.126百万年前)冰期褪去之后, 表明更新世气候的波动影响了西江中下游鳤的种群动态。作为鳤可能的重要产卵场, 西江中下游部分江段可以考虑建立鳤的自然保护区, 用于保育和修复鳤的种质资源。

关键词: 鳤, 仔鱼采集, 线粒体基因和核基因, 种群扩张, 保护

Abstract:

Ochetobius elongatus used to be an economically important fish species in many rivers of China. However, due to environmental destruction and human disturbance the resources of this fish species have reduced rapidly, and its populations are in a critically endangered condition. At present, it is very difficult to obtain specimens of O. elongatus, which limits research on this species. We obtained specimens of O. elongatus via larva and adult fish collection. Two mitochondrial genes and two nuclear genes were used to explore the genetic diversity and population demography of O. elongatus in middle and lower Xijiang River. Results showed that the genetic diversity of O. elongatus was low and is in recession, suggesting that O. elongatus might have undergone a genetic bottleneck event. Additionally, population demographic analyses revealed that O. elongatus population expanded during the Late Pleistocene (0.06 and 0.13 Million years ago), following the glacial retreat of the Middle Pleistocene glaciations (0.78-0.126 Million years ago). The results also revealed that Pleistocene climatic fluctuations have influenced the population demography of O. elongatus. Due to its potential as an important spawning ground for O. elongatus, sections of middle and lower Xijiang River should be considered as candidates for creating nature reserves to conserve and restore its genetic resources.

Key words: Ochetobius elongatus, larva fish collection, mitochondrial and nuclear genes, population expansion, conservation