生物多样性 ›› 2016, Vol. 24 ›› Issue (7): 781-790.DOI: 10.17520/biods.2016038

所属专题: 全球气候变化下的海洋生物多样性专辑

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中国近海重要生态建群红藻真江蓠的群体遗传多样性

刘若愚1,2, 孙忠民1,3, 姚建亭1,4, 胡自民1,4,,A;*(), 段德麟1,4,,A;*()   

  1. 1 .中国科学院海洋研究所实验海洋生物学重点实验室, 山东青岛 266071
    2 .中国科学院大学, 北京 100049
    3 .中国科学院海洋研究所海洋生物分类与系统演化实验室, 山东青岛 266071
    4 .青岛海洋科学与技术国家重点实验室, 山东青岛 266237
  • 收稿日期:2016-02-03 接受日期:2016-06-07 出版日期:2016-07-20 发布日期:2016-08-04
  • 通讯作者: 胡自民,段德麟
  • 基金资助:
    国家自然科学基金(31370264)

Genetic diversity of the habitat-forming red alga Gracilaria vermiculophylla along Chinese coasts

Ruoyu Liu1,2, Zhongmin Sun1,3, Jianting Yao1,4, Zimin Hu1,4,*(), Delin Duan1,4,*()   

  1. 1 Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong 266071
    2 University of Chinese Academy of Sciences, Beijing 100049
    3 Department of Marine Organism Taxonomy and Phylogeny, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong 266071
    4 Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong 266237
  • Received:2016-02-03 Accepted:2016-06-07 Online:2016-07-20 Published:2016-08-04
  • Contact: Hu Zimin,Duan Delin

摘要:

真江蓠(Gracilaria vermiculophylla)是中国近海潮间带生态系统结构组成和功能维持的重要支撑物种, 但有关其群体遗传结构和多样性分布模式的研究目前仍较缺乏。本研究利用线粒体cox1序列对我国近海19个真江蓠地理群体进行了系统发育和群体遗传分析。461个长度为641 bp的cox1序列片段共含有21个多态位点, 产生15个单倍型。基于cox1序列的系统进化分析、单倍型分析和主成分分析显示, 19个真江蓠群体分化为南北两个类群, 其中浙江嵊泗以北的13个群体形成北方类群, 福建厦门以南的6个群体形成南方类群。遗传距离和分子方差分析显示真江蓠南北各类群内的遗传分化较小, 南北类群间的遗传分化达到亚种水平。南北类群间的差异是我国近海真江蓠群体遗传变异的主要来源。

关键词: Gracilaria vermiculophylla, 线粒体cox1, 系统发育, 遗传多样性, 群体遗传分化

Abstract:

As a habitat-forming species, the red alga Gracilaria vermiculophylla has been noted to play an essential role in shaping coastal marine communities and maintaining intertidal ecosystems along Chinese coasts. However, few studies have been conducted that analyze the genetic structure of the population and the distribution patterns of genetic variation. In this study, we compiled partial mitochondrial DNA cytochrome c oxidase subunit I gene (cox1) of 19 G. vermiculophylla populations (461 individuals) and performed phylogenetic analysis and population genetic surveys. A total of 461 mt-DNA cox1 sequences were obtained and edited into 641 bp, which contained 21 polymorphic sites and yielded 15 haplotypes. Phylogenetic inferences, haplotype networking, and principal coordinate analysis consistently indicated that G. vermiculophylla populations diverged into two groups: the northern group is comprised of specimens from the Yellow-Bohai Sea and the Shengsi Islands, while the southern group is comprised of specimens from six locations south to Xiamen, Fujian Province. Analysis of the molecular variance and Nei’s genetic distances indicated low genetic differentiation between populations in each group while group-level genetic divergence is comparable to the degree of subspecies differentiation.

Key words: Gracilaria vermiculophylla, mitochondrial cox1, phylogenetics, genetic diversity, population genetic differentiation