生物多样性 ›› 2010, Vol. 18 ›› Issue (3): 241-250.DOI: 10.3724/SP.J.1003.2010.241

• 论文 • 上一篇    下一篇

基于rDNA ITS序列探讨粉煤灰污染对萼花臂尾轮虫种群遗传多样性的影响

张根1, 席贻龙1,*(), 薛颖昊1, 胡忻2, 项贤领1, 温新利1   

  1. 1 安徽师范大学生命科学学院; 安徽省高校生物环境与生态安全省级重点实验室, 安徽芜湖 241000
    2 南京大学现代分析测试中心, 江苏南京 210093
  • 收稿日期:2010-02-02 接受日期:2010-05-11 出版日期:2010-05-20 发布日期:2012-02-08
  • 通讯作者: 席贻龙
  • 作者简介: E-mail: ylxi1965@yahoo.com.cn
  • 基金资助:
    国家自然科学基金(30770352);国家自然科学基金(30870369);安徽省优秀青年基金滚动资助项目(08040106904);安徽省重要生物资源的保护和利用研究重点实验室专项基金资助项目;安徽省教育厅自然科学基金项目(KJ2009B089Z);安徽省高等学校优秀青年基金(2009SQRZ029)

Effects of coal ash pollution on the genetic diversity of Brachionus calyciflorus based on rDNA ITS sequences

Gen Zhang1, Yilong Xi1,*(), Yinghao Xue1, Xin Hu2, Xianling Xiang1, Xinli Wen1   

  1. 1 Provincial Key Laboratory of Biotic Environment & Ecological Safety in Anhui, College of Life Sciences, Anhui Normal University, Wuhu, Anhui 241000
    2 Center of Modern Analysis, Nanjing University, Nanjing 210093
  • Received:2010-02-02 Accepted:2010-05-11 Online:2010-05-20 Published:2012-02-08
  • Contact: Yilong Xi

摘要:

本文通过rDNA ITS序列测定和分析, 比较研究了粉煤灰污染水体(灰湖)与未污染水体(凤鸣湖和汀棠湖)萼花臂尾轮虫(Brachionus calyciflorus)的种群遗传多样性。结果表明, 灰湖萼花臂尾轮虫仅有1个姐妹种, 而两对照湖泊均存在2个姐妹种, 粉煤灰污染使萼花臂尾轮虫种复合体内姐妹种数量减少。就三个湖泊共有的姐妹种I而言, 灰湖轮虫种群单倍型多样性最低(h= 0.9516), 核苷酸多样性(π = 0.0066)低于汀棠湖(π = 0.0073)却高于凤鸣湖种群(π = 0.0052), 粉煤灰污染对萼花臂尾轮虫种群遗传多样性表现出一定的破坏作用; 凤鸣湖轮虫种群核苷酸多样性最低, 可能是由于水体中晶囊轮虫(Asplanchna)和桡足类动物捕食压力较高以及周围农田污染的联合作用造成的。三湖泊间, 仅汀棠湖与凤鸣湖轮虫种群间基因交流值较低(Nm = 1.95), 种群分化指数较高(Fst = 0.11358); 而灰湖与汀棠湖以及灰湖与凤鸣湖轮虫种群间基因交流水平较高(Nm均大于4), 种群分化指数较低(Fst分别为0.03535和0.00276)。AMOVA分析显示汀棠湖与凤鸣湖轮虫种群间变异所占比例较高, 为12.87%; 而灰湖与汀棠湖以及灰湖与凤鸣湖轮虫种群间变异所占比例较低, 分别为3.78%和2.78%。粉煤灰污染水体与未污染水体萼花臂尾轮虫种群间保持较高水平的基因交流, 种群分化不明显。

关键词: 序列分析, 电厂, 遗传分化, 重金属, 水质分析

Abstract

In this study, rDNA ITS sequences were analyzed to compare the genetic diversity of Brachionus calyciflorus from the coal ash contaminated (Lake Hui) and two uncontaminated lakes (Lake Tingtang and Lake Fengming). The results showed that two sibling species in Brachionus calyciflorus species complex were defined in both Lake Tingtang and Lake Fengming, but only one sibling species was found in Lake Hui. The coal ash pollution decreased the number of sibling species. Based on the sequences of sibling species I coexisting in all the three lakes, the haplotype diversity of the rotifer population from Lake Hui was the lowest (h=0.9516), and the nucleotide diversity of the rotifer population from Lake Hui (π=0.0066) was lower than that from Lake Tingtang (π=0.0073) but higher than that from Lake Fengming (π=0.0052). The lowest nucleotide diversity of the rotifer population from Lake Fengming might be attributed to the combined effects of the pollution from surrounding agricultural fields and higher predation pressure from Asplanchna and copepod. Among the three lakes, a lower Nm (1.95) but a higher Fst (0.11358) were found between rotifer populations from Lake Tingtang and Lake Fengming, but higherNm values (>4) were confirmed between the rotifer populations from Lake Hui and Lake Tingtang as well as Lake Hui and Lake Fengming, with the lowerFst values of 0.03535 and 0.00276, respectively. AMOVA indicated that a higher percentage of variation (12.87%) was found between the populations from Lake Tingtang and Lake Fengming, but lower percentages (3.78% and 2.78%) were evaluated between the populations from Lake Hui and Lake Tingtang as well as Lake Hui and Lake Fengming. There was a moderate level of gene exchange, but no obvious differentiation between the populations from coal ash polluted and unpolluted lakes.

Key words: sequence analysis, power plant, genetic differentiation, heavy metal, water quality analysis