生物多样性 ›› 2010, Vol. 18 ›› Issue (1): 76-82.DOI: 10.3724/SP.J.1003.2010.076

• 研究报告 • 上一篇    下一篇

银叶杜鹃和繁花杜鹃根部真菌的多样性

郑钰1,3, 高博4, 孙立夫1,2, 邴艳红1, 裴克全1,*()   

  1. 1 中国科学院植物研究所植被与环境变化国家重点实验室, 北京 100093
    2 绍兴文理学院, 浙江绍兴 312000
    3 中国科学院研究生院, 北京 100049
    4 东北林业大学, 哈尔滨 150040
  • 收稿日期:2009-10-14 接受日期:2009-11-27 出版日期:2010-01-20 发布日期:2010-01-20
  • 通讯作者: 裴克全
  • 作者简介:* E-mail: peikequan@ibcas.ac.cn
  • 基金资助:
    国家自然科学基金委主任基金(30740007);国家自然科学基金重点项目(30930005)

Diversity of fungi associated with Rhododendron argyrophyllum and R. floribundum hair roots in Sichuan, China

Yu Zheng1,3, Bo Gao4, Lifu Sun1,2, Yanhong Bing1, Kequan Pei1,*()   

  1. 1 State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093
    2 Life & Science Faculty, Shaoxing College of Arts & Science, Shaoxing, Zhejiang 312000
    3 Graduate University of the Chinese Academy of Sciences, Beijing 100049
    4 Northeast Forestry University, Harbin 150040
  • Received:2009-10-14 Accepted:2009-11-27 Online:2010-01-20 Published:2010-01-20
  • Contact: Kequan Pei

摘要:

植物根系与真菌形成菌根, 在自然生态系统的物质能量循环中具有重要的生态功能。作者在四川省的中国杜鹃园选取银叶杜鹃(Rhododendron argyrophyllum)和繁花杜鹃(R. floribundum), 通过直接扩增杜鹃花根部真菌rDNA-ITS区片段, 来揭示该地区杜鹃花属植物根部真菌的多样性。ITS序列分析结果表明: 从两种杜鹃的根部共检测到41个真菌分类单元, 分别属于子囊菌纲的柔膜菌目(Helotiales)、散囊菌目(Eurotiales)、盘菌目(Pezizales)、假球壳目(Pleosporales)和担子菌纲的蜡壳耳目(Sebacinales)、伞菌目(Agaricales)、Erythrobasidiales、线黑粉菌目(Filobasidiales)。银叶杜鹃和繁花杜鹃根部真菌种类丰富, 包括了杜鹃花类菌根真菌、外生菌根真菌和其他类型真菌, 其中担子菌纲的蜡壳耳目和子囊菌纲的柔膜菌目占有较大比例。

关键词: Rhododendron, 杜鹃花类菌根, 真菌多样性, ITS

Abstract

We investigated the fungal associates of Rhododendron argyrophyllum and R. floribundum in Sichuan Province. A total of 41 fungal taxa were identified from hair roots based on ITS sequence analyses belonging to the following orders: Helotiales, Eurotiales, Pezizales, and Pleosporales in Ascomycetes, Sebacinales and Agaricales, Erythrobasidiales, and Filobasidiales in Basidiomycetes. Fungal diversity was rich, including ericoid mycorrhizal (ERM), ectomycorrhizal (ECM) and other root-associated fungi. Sebacinales and Helotiales occupied a high proportion of total fungal samples.

Key words: ericoid mycorrhizal fungi, fungal diversity, ITS, Rhododendron