生物多样性 ›› 2016, Vol. 24 ›› Issue (3): 296-303.DOI: 10.17520/biods.2015259

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基于ITS和ndhF-rpl32序列的荞麦种间亲缘关系分析

胡亚妮1,2, 张宗文2,3,*(), 吴斌2, 高佳2, 李艳琴1,*()   

  1. 1 山西大学生物技术研究所, 太原 030006
    2 中国农业科学院作物科学研究所, 北京 100081
    3 国际生物多样性中心中国办事处, 北京 100081
  • 收稿日期:2015-09-21 接受日期:2016-03-02 出版日期:2016-03-20 发布日期:2016-04-05
  • 通讯作者: 张宗文,李艳琴
  • 基金资助:
    特色小宗作物种质资源发掘与创新利用(2013BAD01B05-2)和作物种质资源保护与利用专项(2014NWB030-06)

Genetic relationships of buckwheat species based on the sequence analysis of ITS and ndhF-rpl32

Yani Hu1,2, Zongwen Zhang2,3,*(), Bin Wu2, Jia Gao2, Yanqin Li1,*()   

  1. 1 Institute of Biotechnology, Shanxi University, Taiyuan 030006
    2 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081
    3 China Office of Biodiversity International, Beijing 100081
  • Received:2015-09-21 Accepted:2016-03-02 Online:2016-03-20 Published:2016-04-05
  • Contact: Zhang Zongwen,Li Yanqin

摘要:

荞麦起源于我国, 演化形成了丰富的物种和遗传多样性。为了有效研究和利用荞麦及其野生种资源, 以从四川、甘肃、贵州等地采集的荞麦属(Fagopyrum)10个种(含变种、亚种和复合体种)共71份材料为对象, 通过ITS和叶绿体ndhF-rpl32序列分析, 利用MEGA5.0构建系统进化树, 探讨了荞麦种内及种间亲缘关系。结果表明, 在ITS序列矩阵中, 序列长度为725 bp, 信息位点为150个, 占序列总长度的20.7%; 在ndhF-rpl32序列矩阵中, 序列长度为940 bp, 信息位点为158个, 占序列总长度的16.8%。由ITS序列和ndhF-rpl32序列构建的两个进化树都可以将71份材料分为大粒荞麦种组和小粒荞麦种组; 其中, 大粒荞麦种组包括栽培苦荞和米苦荞(F. tataricum)、金荞复合体(F. cymosum complex)、栽培甜荞(F. esculentum)和野生甜荞(F. esculentum ssp. ancestralis); 小粒荞麦种组包括齿翅野荞(F. gracilipes var. odontopterum)、疏穗小野荞(F. leptopodum var. grossii)、小野荞(F. leptopodum)、密毛野荞(F. densovillosum)、细柄野荞(F. gracilipes)和硬枝万年荞(F. urophyllum)。而ndhF-rpl32序列构建的系统发育树还能区分栽培甜荞和野生甜荞, 具有更好的聚类效果。另外, 与栽培甜荞相比, 金荞复合体与栽培苦荞的亲缘关系更近。该研究为荞麦属种的分类和条形码研究提供了一定的科学依据。

关键词: Fagopyrum, 亲缘关系, ITS, ndhF-rpl32

Abstract

Buckwheat was originated in China and evolved into abundant species and genetic diversity. In order to effectively use germplasm resources of buckwheat and its wild relatives, the genetic relationships of buckwheat species were analyzed on 71 Fagopyrum samples including 10 species (subspecies, varieties and complex), which were collected mainly from Sichuan, Guizhou and Gansu provinces. Through the analysis of ITS and ndhF-rpl32 sequences and the construction of phylogenetic trees, the inter- and intra-specific relationships of these species were discussed. Results showed that the sequence length of ITS was 725 bp in the matrix, while the parsimony informative sites was 150, accounting for 20.7% of the total length. In the matrix of ndhF-rpl32 sequence, the length was 940 bp, while the parsimony informative sites was 158, accounting for 16.8% of total length. On one hand, the phylogenetic trees based on both ITS and ndhF-rpl32 sequencing data clearly separated the big-achene-group buckwheat (F. tataricum, F. cymosum complex, F. esculentum and F. esculentum ssp. ancestralis) from the small-achene-group buckwheat (F. gracilipes var. odontopterum, F. leptopodum var. grossii, F. leptopodum, F. densovillosum, F. gracilipes, and F. urophyllum). On the other hand, the phylogenetic tree based on ndhF-rpl32 sequences can be used to distinguish F. esculentum ssp. ancestralis and F. esculentum, which indicated that the ndhF-rpl32 sequence was more effective in analyzing the phylogenetic relationships of buckwheat species. These results provide a scientific basis for studies of taxonomy and establishing barcodes of Fagopyrum species.

Key words: buckwheat, genetic relationships, ITS, ndhF-rpl32