生物多样性 ›› 2003, Vol. 11 ›› Issue (1): 10-19.DOI: 10.17520/biods.2003002

• 论文 • 上一篇    下一篇

Trichoderma harzianum及其近缘种的分子系统学研究

章初龙, 徐同*   

  1. (浙江大学农业与生物技术学院生物技术研究所,杭州 310029)
    (中国科学院微生物研究所真菌地衣系统学开放研究实验室,北京 100080)
  • 收稿日期:2002-03-19 修回日期:2002-06-22 出版日期:2003-01-20 发布日期:2003-01-20
  • 通讯作者: 徐 同

Molecular phylogenetic analysis of Trichoderma harzianum and its related species

ZHANG Chu Long, XU Tong*   

  1. 1 Institute of Biotechnology,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310029
    2 Systematic Mycology & Lichenology Laboratory,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100080
  • Received:2002-03-19 Revised:2002-06-22 Online:2003-01-20 Published:2003-01-20
  • Contact: XU Tong

摘要: Trichoderma harzianum是木霉属内最常见的一个“集合种”。本研究对来源不同的T.harzianum及其相似种的46个菌株进行了ITS序列测定,将其ITS1-5.8S-ITS2序列与来自EMBL的参考菌株的序列进行比较,并进行系统发育分析,此外对其中的18个菌株进行了RAPD多态性分析,试图明确T. harzianum 的多样性以及与其相似种之间的关系。ITS结果表明,T. harzianum及其相似种可分成2个群(A、B):A群由T.hamatumT.asperellumT. atrovirideT.koningiiT.viride组成,并形成 2个分支,表明T.virideT.koningiiT. atroviride的亲缘关系较近,而与T.hamatumT.asperellum较远;B群由T.spiraleT.hamatumT.inhamatumT.harzianumT.anam.Hypocrea vinosa组成,并形成6个分支。T.inhamatum可分成2个群(Ti1、Ti2)、T.harzianum至少可分成5个群(Th1、Th2、Th4、Th5、Th6)。结果还表明T.hamatum的遗传差异较大,T. hamatum的模式菌株归属于A群,而其他的T.hamatum的菌株归属于B群。RAPD结果与ITS的结果基本一致。

AbstractTrichoderma harzianum is one of the most common aggregate species of the genus. Based on different molecular characterizations, strains of T. harzianum have been divided into different subgroups, which has created confusion. We reexamined reference strains representing different subgroups under the name T. harzianum and its related species. Phylogenetic analysis using the NEIGHBOR program in the PHYLIP package based on ITS1 5.8S rDNA ITS2 sequence data distributed the tested strains among two phylogenetic groups (A and B), which was supported by high bootstrap values. Group A contains T. hamatum , T.asperellum, T. atroviride, T.koningii and T.viride , and is divided into two clades. T.viride is more closely related to T.koningii and T.atroviride than to T. hamatum and T. asperellum . Group B contains T. spirale , T. hamatum , T. inhamatum , T. harzianum and T. anam. Hypocrea vinosa , and is divided into six clades. T. inhamatum is further divided into two subgroups (Ti1 and Ti2), and T. harzianum comprises at least five subgroups(Th1, Th2, Th4, Th5 and Th6). The phylogenetic analysis of ITS1 5.8S rDNA ITS2 sequence also indicated the heterogeneity of T. hamatum ,since a neotype of T. hamatum is located in group A but other strains of T. hamatum are located in group B. The RAPD analysis of 18 strains representing T. harzianum and its related species coincided with phylogeny based on sequence analysis of the ITS regions.