银叶杜鹃和繁花杜鹃根部真菌的多样性

doi:10.3724/SP.J.1003.2010.076

生物多样性 ›› 2010, Vol. 18 ›› Issue (1): 76-82. DOI: 10.3724/SP.J.1003.2010.076

银叶杜鹃和繁花杜鹃根部真菌的多样性

郑钰¹^,³, 高博⁴, 孙立夫¹^,², 邴艳红¹, 裴克全¹^,^*()

1 中国科学院植物研究所植被与环境变化国家重点实验室, 北京 100093
2 绍兴文理学院, 浙江绍兴 312000
3 中国科学院研究生院, 北京 100049
4 东北林业大学, 哈尔滨 150040

收稿日期:2009-10-14 接受日期:2009-11-27 出版日期:2010-01-20 发布日期:2010-01-20
通讯作者: 裴克全
作者简介:* E-mail: peikequan@ibcas.ac.cn
基金资助:
国家自然科学基金委主任基金(30740007);国家自然科学基金重点项目(30930005)

Diversity of fungi associated with Rhododendron argyrophyllum and R. floribundum hair roots in Sichuan, China

Yu Zheng¹^,³, Bo Gao⁴, Lifu Sun¹^,², Yanhong Bing¹, Kequan Pei¹^,^*()

1 State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093
2 Life & Science Faculty, Shaoxing College of Arts & Science, Shaoxing, Zhejiang 312000
3 Graduate University of the Chinese Academy of Sciences, Beijing 100049
4 Northeast Forestry University, Harbin 150040

Received:2009-10-14 Accepted:2009-11-27 Online:2010-01-20 Published:2010-01-20
Contact: Kequan Pei

摘要/Abstract

摘要：

植物根系与真菌形成菌根, 在自然生态系统的物质能量循环中具有重要的生态功能。作者在四川省的中国杜鹃园选取银叶杜鹃(Rhododendron argyrophyllum)和繁花杜鹃(R. floribundum), 通过直接扩增杜鹃花根部真菌rDNA-ITS区片段, 来揭示该地区杜鹃花属植物根部真菌的多样性。ITS序列分析结果表明: 从两种杜鹃的根部共检测到41个真菌分类单元, 分别属于子囊菌纲的柔膜菌目(Helotiales)、散囊菌目(Eurotiales)、盘菌目(Pezizales)、假球壳目(Pleosporales)和担子菌纲的蜡壳耳目(Sebacinales)、伞菌目(Agaricales)、Erythrobasidiales、线黑粉菌目(Filobasidiales)。银叶杜鹃和繁花杜鹃根部真菌种类丰富, 包括了杜鹃花类菌根真菌、外生菌根真菌和其他类型真菌, 其中担子菌纲的蜡壳耳目和子囊菌纲的柔膜菌目占有较大比例。

关键词: Rhododendron, 杜鹃花类菌根, 真菌多样性, ITS

Abstract

We investigated the fungal associates of Rhododendron argyrophyllum and R. floribundum in Sichuan Province. A total of 41 fungal taxa were identified from hair roots based on ITS sequence analyses belonging to the following orders: Helotiales, Eurotiales, Pezizales, and Pleosporales in Ascomycetes, Sebacinales and Agaricales, Erythrobasidiales, and Filobasidiales in Basidiomycetes. Fungal diversity was rich, including ericoid mycorrhizal (ERM), ectomycorrhizal (ECM) and other root-associated fungi. Sebacinales and Helotiales occupied a high proportion of total fungal samples.

Key words: ericoid mycorrhizal fungi, fungal diversity, ITS, Rhododendron

郑钰, 高博, 孙立夫, 邴艳红, 裴克全 (2010) 银叶杜鹃和繁花杜鹃根部真菌的多样性. 生物多样性, 18, 76-82. DOI: 10.3724/SP.J.1003.2010.076.

Yu Zheng, Bo Gao, Lifu Sun, Yanhong Bing, Kequan Pei (2010) Diversity of fungi associated with Rhododendron argyrophyllum and R. floribundum hair roots in Sichuan, China. Biodiversity Science, 18, 76-82. DOI: 10.3724/SP.J.1003.2010.076.

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链接本文: https://www.biodiversity-science.net/CN/10.3724/SP.J.1003.2010.076

https://www.biodiversity-science.net/CN/Y2010/V18/I1/76

图/表 3

参考文献 33

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克隆 Clone	序列号 GenBank accession no.	BLAST最接近的比对 Closest BLAST match	重叠区 Overlap (bp)	相似度 Identity (%)	克隆数目 Clone No.
克隆 Clone	序列号 GenBank accession no.	BLAST最接近的比对 Closest BLAST match	重叠区 Overlap (bp)	相似度 Identity (%)	RA		RF
子囊菌纲 Ascomycota
061229	GU256941	Alternaria alternate (DQ023279)	608	100	1	3
061227¹	GU256942	—	—	—	1	0
061266	GU256943	Uncultured Geoglossaceae clone Y43 (DQ273321)	565	90.2	8	6
061626	GU256944	Uncultured Rhizoscyphus ericae complex clone K10-16 (AY394687)	591	93.3	1	5
061206	GU256945	Uncultured Rhizoscyphus ericae complex clone K10-16 (AY394687)	550	92.5	7	12
061619	GU256946	Rhizoscyphus ericae aggregate (AM084704)	573	94.5	0	2
061239	GU256947	Rhizoscyphus ericae aggregate (AY394684)	550	87.1	3	0
061286	GU256948	Oidiodendron maius strain UAMH 8921 (AF062800)	527	99.1	9	0
061210	GU256949	Cryptosporiopsis ericae voucher UAMH 10419 (AY853167)	599	88.1	1	0
061235	GU256950	Phialea strobilina strain CBS 643.85 (EF596821)	571	88.3	3	0
061214	GU256951	Salal root associated fungus UBCtra1011.13 (AF300726)	367	99.2	1	0
061655	GU256952	Mycorrhizal ascomycete of Rhododendron type 3 (AB089663)	522	93.3	0	4
061223	GU256953	Mycorrhizal ascomycete of Rhododendron type 2 (AB089660)	521	95.4	1	0
061245	GU256954	Mycorrhizal ascomycete of Rhododendron type 2 (AB089660)	530	80.8	4	0
061289	GU256955	Penicillium griseofulvum (AB369901)	577	99.6	12	0
061642	GU256956	Penicillium sp. 5/97-5 (AJ279476)	615	99.1	2	2
061211	GU256957	Paecilomyces sp. JCM 12545 (AB217857)	632	100	1	0
担子菌纲 Basidiomyota
061221	GU256958	Erythrobasidium hasegawianum strain CBS 8253 (AF444522)	617	85.5	1	0
061284	GU256959	Erythrobasidium hasegawianum strain CBS 8253 (AF444522)	635	81.6	1	0
061230	GU256960	Sporobolomyces yunnanensis (AB030353)	601	99.1	10	0
061277	GU256961	Uncultured ectomycorrhiza (Basidiomycota) (AY641466)	701	91.1	4	0
061201	GU256962	Cryptococcus terricola strain CBS 6435 (AF444377)	645	100	2	0
061241	GU256963	Cryptococcus sp. BF73 (AM901689)	631	98.3	1	0
061624	GU256964	Uncultured cf. Tricholoma sp. clone d485.8 (AY254876)	542	79.4	0	14
061265	GU256965	Uncultured cf. Tricholoma sp. clone d485.8 (AY254876)	542	82.4	2	0
061647	GU256966	Uncultured cf. Tricholoma sp. clone d485.8 (AY254876)	536	89.7	0	3
061692	GU256967	Uncultured cf. Tricholoma sp. clone d485.8 (AY254876)	542	80.8	0	1
061632	GU256968	Uncultured cf. Tricholoma (AY097046)	670	98.5	0	2
061628	GU256969	Uncultured ectomycorrhiza (Tricholomataceae) (AM113458)	645	85.4	1	20
061610	GU256970	Tricholoma sp. Umpqua (AF377204)	725	82.6	0	1
061207	GU256971	Uncultured mycorrhiza (Sebacinaceae) 4078 (AY634132)	664	84.6	5	0
061220	GU256972	Uncultured mycorrhiza (Sebacinales) voucher 04A16 (DQ352049)	494	88.5	1	0
0616101	GU256973	Uncultured Sebacinales clone R33 (EF030897)	412	93.0	0	1
061203	GU256974	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	674	92.8	2	20
061209	GU256975	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	674	91.6	2	0
061606	GU256976	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	671	91.6	0	2
061262	GU256977	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	673	90.6	4	0
061607	GU256978	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	689	96.3	0	1
061267	GU256979	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	679	92.1	1	0
061668	GU256980	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	669	91.2	0	2
061675	GU256981	Gymnopus austrosemihirtipes AWW65 (AY263422)	796	95.3	0	4

克隆 Clone	序列号 GenBank accession no.	BLAST最接近的比对 Closest BLAST match	重叠区 Overlap (bp)	相似度 Identity (%)	克隆数目 Clone No.
克隆 Clone	序列号 GenBank accession no.	BLAST最接近的比对 Closest BLAST match	重叠区 Overlap (bp)	相似度 Identity (%)	RA		RF
子囊菌纲 Ascomycota
061229	GU256941	Alternaria alternate (DQ023279)	608	100	1	3
061227¹	GU256942	—	—	—	1	0
061266	GU256943	Uncultured Geoglossaceae clone Y43 (DQ273321)	565	90.2	8	6
061626	GU256944	Uncultured Rhizoscyphus ericae complex clone K10-16 (AY394687)	591	93.3	1	5
061206	GU256945	Uncultured Rhizoscyphus ericae complex clone K10-16 (AY394687)	550	92.5	7	12
061619	GU256946	Rhizoscyphus ericae aggregate (AM084704)	573	94.5	0	2
061239	GU256947	Rhizoscyphus ericae aggregate (AY394684)	550	87.1	3	0
061286	GU256948	Oidiodendron maius strain UAMH 8921 (AF062800)	527	99.1	9	0
061210	GU256949	Cryptosporiopsis ericae voucher UAMH 10419 (AY853167)	599	88.1	1	0
061235	GU256950	Phialea strobilina strain CBS 643.85 (EF596821)	571	88.3	3	0
061214	GU256951	Salal root associated fungus UBCtra1011.13 (AF300726)	367	99.2	1	0
061655	GU256952	Mycorrhizal ascomycete of Rhododendron type 3 (AB089663)	522	93.3	0	4
061223	GU256953	Mycorrhizal ascomycete of Rhododendron type 2 (AB089660)	521	95.4	1	0
061245	GU256954	Mycorrhizal ascomycete of Rhododendron type 2 (AB089660)	530	80.8	4	0
061289	GU256955	Penicillium griseofulvum (AB369901)	577	99.6	12	0
061642	GU256956	Penicillium sp. 5/97-5 (AJ279476)	615	99.1	2	2
061211	GU256957	Paecilomyces sp. JCM 12545 (AB217857)	632	100	1	0
担子菌纲 Basidiomyota
061221	GU256958	Erythrobasidium hasegawianum strain CBS 8253 (AF444522)	617	85.5	1	0
061284	GU256959	Erythrobasidium hasegawianum strain CBS 8253 (AF444522)	635	81.6	1	0
061230	GU256960	Sporobolomyces yunnanensis (AB030353)	601	99.1	10	0
061277	GU256961	Uncultured ectomycorrhiza (Basidiomycota) (AY641466)	701	91.1	4	0
061201	GU256962	Cryptococcus terricola strain CBS 6435 (AF444377)	645	100	2	0
061241	GU256963	Cryptococcus sp. BF73 (AM901689)	631	98.3	1	0
061624	GU256964	Uncultured cf. Tricholoma sp. clone d485.8 (AY254876)	542	79.4	0	14
061265	GU256965	Uncultured cf. Tricholoma sp. clone d485.8 (AY254876)	542	82.4	2	0
061647	GU256966	Uncultured cf. Tricholoma sp. clone d485.8 (AY254876)	536	89.7	0	3
061692	GU256967	Uncultured cf. Tricholoma sp. clone d485.8 (AY254876)	542	80.8	0	1
061632	GU256968	Uncultured cf. Tricholoma (AY097046)	670	98.5	0	2
061628	GU256969	Uncultured ectomycorrhiza (Tricholomataceae) (AM113458)	645	85.4	1	20
061610	GU256970	Tricholoma sp. Umpqua (AF377204)	725	82.6	0	1
061207	GU256971	Uncultured mycorrhiza (Sebacinaceae) 4078 (AY634132)	664	84.6	5	0
061220	GU256972	Uncultured mycorrhiza (Sebacinales) voucher 04A16 (DQ352049)	494	88.5	1	0
0616101	GU256973	Uncultured Sebacinales clone R33 (EF030897)	412	93.0	0	1
061203	GU256974	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	674	92.8	2	20
061209	GU256975	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	674	91.6	2	0
061606	GU256976	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	671	91.6	0	2
061262	GU256977	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	673	90.6	4	0
061607	GU256978	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	689	96.3	0	1
061267	GU256979	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	679	92.1	1	0
061668	GU256980	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	669	91.2	0	2
061675	GU256981	Gymnopus austrosemihirtipes AWW65 (AY263422)	796	95.3	0	4

真菌 Fungus	种类数目 No. of ITS taxa	相对频率 Relative frequency¹
真菌 Fungus	种类数目 No. of ITS taxa	RA	RF
Ascomycota
Helotiales	10	28.2	27.6
Eurotiales	3	16.3	1.9
Oidiodendron	1	9.9	0
Other Ascomycetes	3	5.4	2.8
Subtotal	17	59.8	32.3
Basidiomycota
Sebacinales	10	16.3	24.8
Erythrobasidiales	3	13.0	0
Agaricales	9	7.6	42.9
Filobasidiales	2	3.3	0
Subtotal	24	40.2	67.7

真菌 Fungus	种类数目 No. of ITS taxa	相对频率 Relative frequency¹
真菌 Fungus	种类数目 No. of ITS taxa	RA	RF
Ascomycota
Helotiales	10	28.2	27.6
Eurotiales	3	16.3	1.9
Oidiodendron	1	9.9	0
Other Ascomycetes	3	5.4	2.8
Subtotal	17	59.8	32.3
Basidiomycota
Sebacinales	10	16.3	24.8
Erythrobasidiales	3	13.0	0
Agaricales	9	7.6	42.9
Filobasidiales	2	3.3	0
Subtotal	24	40.2	67.7

银叶杜鹃和繁花杜鹃根部真菌的多样性

Diversity of fungi associated with Rhododendron argyrophyllum and R. floribundum hair roots in Sichuan, China

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