生物多样性 ›› 2016, Vol. 24 ›› Issue (8): 932-939.DOI: 10.17520/biods.2016096

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Metabarcoding技术在真菌多样性研究中的应用

曹云1,2, 沈文静2, 陈炼3, 胡飞龙2, 周蕾4, 徐海根2,*()   

  1. 1 南京大学生命科学学院, 南京 210093
    2 环境保护部南京环境科学研究所国家环境保护生物安全重点实验室, 南京 210042
    3 江苏第二师范学院生命科学与化学化工学院, 南京 210013
    4 南京林业大学林学院, 南京 210037
  • 收稿日期:2016-04-01 接受日期:2016-05-27 出版日期:2016-08-20 发布日期:2016-09-02
  • 通讯作者: 徐海根
  • 基金资助:
    国家自然科学基金(31500455)、中国博士后面上基金(2015M571663)和环保公益性行业科研专项(201409061)

Application of metabarcoding technology in studies of fungal diversity

Yun Cao1,2, Wenjing Shen2, Lian Chen3, Feilong Hu2, Lei Zhou4, Haigen Xu2,*()   

  1. 1 School of Life Sciences, Nanjing University, Nanjing 210093
    2 State Environmental Protection Key Laboratory of Biosafety, Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042
    3 College of Life Sciences, Chemistry and Chemical Engineering, Jiangsu Second Normal University, Nanjing 210013
    4 College of Forestry, Nanjing Forestry University, Nanjing 210037
  • Received:2016-04-01 Accepted:2016-05-27 Online:2016-08-20 Published:2016-09-02
  • Contact: Xu Haigen

摘要:

由于受到气候变化、土地利用变化及环境污染等诸多因素的干扰, 真菌多样性受到不容忽视的威胁, 亟需得到保护。构建物种数据库是实现真菌多样性研究和保护的重要前提。近年来兴起的DNA条形码及metabarcoding技术能够在很大程度上弥补传统鉴定方法的缺陷, 可对真菌物种进行大规模、准确、快速、高效地鉴定。本文梳理了metabarcoding技术在真菌物种多样性评估、真菌多样性影响机制和真菌古生态重建等研究中的应用, 同时强调了metabarcoding技术用于真菌多样性研究尚处于初期阶段, 在构建有效参照数据库、优化实验流程以及升级生物信息学工具等方面仍需要进一步的完善。建议加强真菌分类学家、生态学家以及计算机工具研发工程师之间的合作, 共同解决metabarcoding技术在真菌多样性研究及应用中面临的问题, 为宏观尺度上真菌多样性保护提供更加科学的依据。

关键词: metabarcoding, DNA条形码, 真菌多样性保护, 物种鉴定, 高通量测序

Abstract:

Fungal diversity is threatened by climate change, land-use change, and environmental pollution, and requires urgent conservation action. Construction of the fungal species database is an important prerequisite for the study and conservation of fungal diversity. Recently developed DNA barcoding and metabarcoding technologies can provide accurate, rapid, and highly efficient identification on a large scale, and to a large extent compensate for the defects of traditional identification methods. In this paper, we review the application of metabarcoding in fungal species diversity assessment, the study of mechanisms underlying fungal diversity, and the reconstruction of fungal palaeoecology. We emphasize that the application of metabarcoding technology in fungal diversity studies is still in the primary phase, and greater efforts are needed in the construction of reliable reference databases, the optimization of experimental procedures, and updates of bioinformatics tools. Hence, we suggest enhancing cooperation among fungal taxonomists, ecologists, and computer technicians. They should work together to address problems in fungal diversity studies via metabarcoding, which would provide more sound scientific evidence for fungal diversity conservation on a large scale.

Key words: metabarcoding, DNA barcoding, fungal diversity conservation, species identification, high- throughput sequencing