Four species, Tetrataenium wallichii, Quercus baloot, Clinopodium nepalense and Garnotia emodi are reported as newly recorded species in China. Twenty-four species, Pleurospermum decurrens, Hoya linearis, Gynura nepalensis, Solidago decurrens, Euonymus vaganoides, Momordica subangulata subsp. renigera, Zehneria japonica, Elaeagnus sarmentosa, Crotalaria tetragona, Flemingia macrophylla, Coleus forskohlii, Litsea taronensis, Bulbophyllum purpureofuscum, Bulbophyllum repens, Cephalantheropsis obcordata, Dendrobium forrestii, Diglyphosa latifolia, Gastrochilus yunnanensis, Liparis assamica, Otochilus porrectus, Petrosavia sakuraii, Gynochthodes lacunosa, Alectra avensis and Alniphyllum fortunei are reported as newly recorded species in Xizang. Notably, the family Petrosaviaceae as well as the genera Solidago, Zehneria, Flemingia, Coleus, Diglyphosa, Petrosavia, Gynochthodes, Alectra and Alniphyllum have not been previously recorded in Xizang and are reported herein. The voucher specimens for each reported species were collected and deposited in the Herbarium of Kunming Institute of Botany (KUN), Chinese Academy of Sciences.