Biodiv Sci ›› 2022, Vol. 30 ›› Issue (9): 22103.  DOI: 10.17520/biods.2022103

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Gut bacterial composition of four leopard cat subpopulations in Beijing

Fan Xia1, Jing Yang1, Jian Li2, Yang Shi3, Lixin Gai4, Wenhua Huang5, Jingwei Zhang4, Nan Yang6, Fuli Gao1, Yingying Han1, Weidong Bao1,*()   

  1. 1. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083
    2. Miyun District Forestry and Parks Bureau of Beijing Municipality, Beijing 101599
    3. Beijing Wildlife Rescue and Rehabilitation Center, Beijing 100029
    4. Beijing Songshan National Nature Reserve Administration, Beijing 102115
    5. Zhenzhuquan Township Ecological Environment Management Center of Yanqing District of Beijing Municipality, Beijing 102107
    6. Beijing Baihuashan National Nature Reserve Administration, Beijing 102311
  • Received:2022-03-09 Accepted:2022-07-11 Online:2022-09-20 Published:2022-09-09
  • Contact: Weidong Bao
  • About author:First author contact:# Co-first author


Aims: The composition of gut microbiota of wild animals is most influenced by food types and genetic background. This study aimed to investigate gut bacterial composition of leopard cat (Prionailurus bengalensis) and its influencing factors in Beijing.
Methods: In this study, fecal samples of leopard cat collected from Yunmengshan, Yunfengshan, Songshan and Baihuashan were analyzed by high-throughput sequencing on bacterial 16S rRNA hypervariable region of V3-V4.
Results: The results showed that the dominant flora of gut bacteria of leopard cat in phyla included Firmicutes (relative abundance 52.40%), Proteobacteria (25.18%), Actinobacteria (9.07%), Bacteroidetes (8.17%), and Fusobacteria (4.74%). The top 5 genera of bacteria with the highest abundance accounted for about 50% of the total abundance, which were Pseudomonas (13.37%), Blautia (11.20%), Clostridium_sensu_stricto_1 (9.10%), Peptoclostridium (8.62%), and Lactobacillus (6.08%). There was no significant difference in gut bacterial abundance and β diversity among the leopard cat subpopulations in four sampling areas, but the ACE index and Chao 1 index in Songshan subpopulation were different from those in Yunmengshan and Yunfengshan.
Conclusion: Considering there was a disparity trend in genetic structure in Songshan subpopulation, and the climate types of the four areas and food composition of the leopard cat were highly similar, we postulated that the gut bacteria of leopard cat was mainly influenced by the genetic traits for local adaptation.

Key words: leopard cat, gut bacteria, subpopulation, high-throughput sequencing, Beijing