Biodiv Sci ›› 2023, Vol. 31 ›› Issue (6): 22659.  DOI: 10.17520/biods.2022659

• Original Papers: Microbial Diversity • Previous Articles     Next Articles

Impact of Phyllostachys edulis expansion to Chinese fir forest on the soil bacterial community

Yinger Mao1, Xiumei Zhou2, Nan Wang2, Xiuxiu Li1, Yuke You1, Shangbin Bai1,2,*()   

  1. 1. Zhejiang Provincial Key Laboratory of Carbon Cycling in Forest Ecosystems and Carbon Sequestration, Zhejiang A&F University, Hangzhou 311300
    2. Jiyang College, Zhejiang A&F University, Zhuji, Zhejiang 311800
  • Received:2022-11-25 Accepted:2023-04-03 Online:2023-06-20 Published:2023-04-25
  • Contact: * E-mail:


Aims: Soil microorganisms are an important component of forest ecosystems, and the expansion of bamboo (Phyllostachys edulis) causes changes in vegetation types, thereby affecting soil microbial communities. The purpose of this study is to reveal the impact of bamboo expansion into Chinese fir forests on soil bacterial communities.

Methods: In Nanshan Village, Yuqian Town, Lin’an District, Hangzhou City, Zhejiang Province, with pure Chinese fir (Cunninghamia lanceolata) forest (s) as the control, two kinds of stands of mixed bamboo and Chinese fir forest (h) and pure bamboo forest (m) were selected to explore the impact of bamboo expansion on soil bacterial communities of Chinese fir forest through high-throughput sequencing techniques.

Results: The expansion of bamboo led to a decrease in the alpha diversity of soil bacterial communities in Chinese fir forests. Compared with Chinese fir forests, the bacterial diversity and richness in mixed forests was decreased by 2.4% and 8.6%, but there was no significant difference, and in moso bamboo forest land was decreased by 5.9% and 16%, with significant differences (P < 0.05). After the expansion of Chinese fir forest by bamboo, the relative abundance of soil bacterial flora was changed; The relative abundance of Proteobacteria, Unassigned, and Verrucomicrobia in Chinese fir forests was the highest, accounting for 32.39%, 3.7%, and 3.2%, respectively; The relative abundance of Acidobacteria and Bacteroides in bamboo forests was the highest, accounting for 45.05% and 5.19%, respectively; The relative abundance of Planctomycotes, Actinobacteria, and Chloroflexi in the mixed forest was the highest, accounting for 7.51%, 6.79%, and 5.12%, respectively. After the expansion of bamboo into a mixed forest, the relative abundance of Planctomycotes and Actinobacteria increased significantly (P < 0.05), increasing by 3.12% and 2.71%. The relative abundance of Elusimicrobia and Germatimonadetes decreased significantly (P < 0.05), decreasing by 0.43% and 0.36%; The relative abundance of Thaumarchaeota decreased significantly (P < 0.05). The beta diversity analysis results showed that bamboo expansion had a significant impact on the soil bacterial community structure of Chinese fir forests (P < 0.05). The soil bacterial structure of bamboo forests and bamboo fir mixed forests was grouped together and separate from the Chinese fir forests. LEfSe analysis showed that the relative abundance of Nitrospira, Gemmatimonadaceae, and Mycobacterium could be used as an indicator for the changes in soil bacterial community structure in the expanded fir forest of bamboo. Soil NH4+ may be the main soil environmental factor that causes changes in bacterial communities.

Conclusion: These results indicate that the expansion of bamboo into moso Chinese fir forests can cause changes in the soil bacterial community in the forest land, providing basic data for understanding the cycling of soil carbon and nitrogen, expansion mechanism, and expansion regulation of bamboo.

Key words: moso bamboo expansion, Chinese fir forest, bacterial community structure, high-throughput sequencing techniques