Diversity of fungi associated with Rhododendron argyrophyllum and R. floribundum hair roots in Sichuan, China

doi:10.3724/SP.J.1003.2010.076

Abstract

Abstract:

We investigated the fungal associates of Rhododendron argyrophyllum and R. floribundum in Sichuan Province. A total of 41 fungal taxa were identified from hair roots based on ITS sequence analyses belonging to the following orders: Helotiales, Eurotiales, Pezizales, and Pleosporales in Ascomycetes, Sebacinales and Agaricales, Erythrobasidiales, and Filobasidiales in Basidiomycetes. Fungal diversity was rich, including ericoid mycorrhizal (ERM), ectomycorrhizal (ECM) and other root-associated fungi. Sebacinales and Helotiales occupied a high proportion of total fungal samples.

Key words: ericoid mycorrhizal fungi, fungal diversity, ITS, Rhododendron

Yu Zheng, Bo Gao, Lifu Sun, Yanhong Bing, Kequan Pei. Diversity of fungi associated with Rhododendron argyrophyllum and R. floribundum hair roots in Sichuan, China[J]. Biodiv Sci, 2010, 18(1): 76-82.

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URL: https://www.biodiversity-science.net/EN/10.3724/SP.J.1003.2010.076

https://www.biodiversity-science.net/EN/Y2010/V18/I1/76

Figures/Tables 3

References 33

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克隆 Clone	序列号 GenBank accession no.	BLAST最接近的比对 Closest BLAST match	重叠区 Overlap (bp)	相似度 Identity (%)	克隆数目 Clone No.
克隆 Clone	序列号 GenBank accession no.	BLAST最接近的比对 Closest BLAST match	重叠区 Overlap (bp)	相似度 Identity (%)	RA		RF
子囊菌纲 Ascomycota
061229	GU256941	Alternaria alternate (DQ023279)	608	100	1	3
061227¹	GU256942	—	—	—	1	0
061266	GU256943	Uncultured Geoglossaceae clone Y43 (DQ273321)	565	90.2	8	6
061626	GU256944	Uncultured Rhizoscyphus ericae complex clone K10-16 (AY394687)	591	93.3	1	5
061206	GU256945	Uncultured Rhizoscyphus ericae complex clone K10-16 (AY394687)	550	92.5	7	12
061619	GU256946	Rhizoscyphus ericae aggregate (AM084704)	573	94.5	0	2
061239	GU256947	Rhizoscyphus ericae aggregate (AY394684)	550	87.1	3	0
061286	GU256948	Oidiodendron maius strain UAMH 8921 (AF062800)	527	99.1	9	0
061210	GU256949	Cryptosporiopsis ericae voucher UAMH 10419 (AY853167)	599	88.1	1	0
061235	GU256950	Phialea strobilina strain CBS 643.85 (EF596821)	571	88.3	3	0
061214	GU256951	Salal root associated fungus UBCtra1011.13 (AF300726)	367	99.2	1	0
061655	GU256952	Mycorrhizal ascomycete of Rhododendron type 3 (AB089663)	522	93.3	0	4
061223	GU256953	Mycorrhizal ascomycete of Rhododendron type 2 (AB089660)	521	95.4	1	0
061245	GU256954	Mycorrhizal ascomycete of Rhododendron type 2 (AB089660)	530	80.8	4	0
061289	GU256955	Penicillium griseofulvum (AB369901)	577	99.6	12	0
061642	GU256956	Penicillium sp. 5/97-5 (AJ279476)	615	99.1	2	2
061211	GU256957	Paecilomyces sp. JCM 12545 (AB217857)	632	100	1	0
担子菌纲 Basidiomyota
061221	GU256958	Erythrobasidium hasegawianum strain CBS 8253 (AF444522)	617	85.5	1	0
061284	GU256959	Erythrobasidium hasegawianum strain CBS 8253 (AF444522)	635	81.6	1	0
061230	GU256960	Sporobolomyces yunnanensis (AB030353)	601	99.1	10	0
061277	GU256961	Uncultured ectomycorrhiza (Basidiomycota) (AY641466)	701	91.1	4	0
061201	GU256962	Cryptococcus terricola strain CBS 6435 (AF444377)	645	100	2	0
061241	GU256963	Cryptococcus sp. BF73 (AM901689)	631	98.3	1	0
061624	GU256964	Uncultured cf. Tricholoma sp. clone d485.8 (AY254876)	542	79.4	0	14
061265	GU256965	Uncultured cf. Tricholoma sp. clone d485.8 (AY254876)	542	82.4	2	0
061647	GU256966	Uncultured cf. Tricholoma sp. clone d485.8 (AY254876)	536	89.7	0	3
061692	GU256967	Uncultured cf. Tricholoma sp. clone d485.8 (AY254876)	542	80.8	0	1
061632	GU256968	Uncultured cf. Tricholoma (AY097046)	670	98.5	0	2
061628	GU256969	Uncultured ectomycorrhiza (Tricholomataceae) (AM113458)	645	85.4	1	20
061610	GU256970	Tricholoma sp. Umpqua (AF377204)	725	82.6	0	1
061207	GU256971	Uncultured mycorrhiza (Sebacinaceae) 4078 (AY634132)	664	84.6	5	0
061220	GU256972	Uncultured mycorrhiza (Sebacinales) voucher 04A16 (DQ352049)	494	88.5	1	0
0616101	GU256973	Uncultured Sebacinales clone R33 (EF030897)	412	93.0	0	1
061203	GU256974	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	674	92.8	2	20
061209	GU256975	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	674	91.6	2	0
061606	GU256976	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	671	91.6	0	2
061262	GU256977	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	673	90.6	4	0
061607	GU256978	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	689	96.3	0	1
061267	GU256979	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	679	92.1	1	0
061668	GU256980	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	669	91.2	0	2
061675	GU256981	Gymnopus austrosemihirtipes AWW65 (AY263422)	796	95.3	0	4

克隆 Clone	序列号 GenBank accession no.	BLAST最接近的比对 Closest BLAST match	重叠区 Overlap (bp)	相似度 Identity (%)	克隆数目 Clone No.
克隆 Clone	序列号 GenBank accession no.	BLAST最接近的比对 Closest BLAST match	重叠区 Overlap (bp)	相似度 Identity (%)	RA		RF
子囊菌纲 Ascomycota
061229	GU256941	Alternaria alternate (DQ023279)	608	100	1	3
061227¹	GU256942	—	—	—	1	0
061266	GU256943	Uncultured Geoglossaceae clone Y43 (DQ273321)	565	90.2	8	6
061626	GU256944	Uncultured Rhizoscyphus ericae complex clone K10-16 (AY394687)	591	93.3	1	5
061206	GU256945	Uncultured Rhizoscyphus ericae complex clone K10-16 (AY394687)	550	92.5	7	12
061619	GU256946	Rhizoscyphus ericae aggregate (AM084704)	573	94.5	0	2
061239	GU256947	Rhizoscyphus ericae aggregate (AY394684)	550	87.1	3	0
061286	GU256948	Oidiodendron maius strain UAMH 8921 (AF062800)	527	99.1	9	0
061210	GU256949	Cryptosporiopsis ericae voucher UAMH 10419 (AY853167)	599	88.1	1	0
061235	GU256950	Phialea strobilina strain CBS 643.85 (EF596821)	571	88.3	3	0
061214	GU256951	Salal root associated fungus UBCtra1011.13 (AF300726)	367	99.2	1	0
061655	GU256952	Mycorrhizal ascomycete of Rhododendron type 3 (AB089663)	522	93.3	0	4
061223	GU256953	Mycorrhizal ascomycete of Rhododendron type 2 (AB089660)	521	95.4	1	0
061245	GU256954	Mycorrhizal ascomycete of Rhododendron type 2 (AB089660)	530	80.8	4	0
061289	GU256955	Penicillium griseofulvum (AB369901)	577	99.6	12	0
061642	GU256956	Penicillium sp. 5/97-5 (AJ279476)	615	99.1	2	2
061211	GU256957	Paecilomyces sp. JCM 12545 (AB217857)	632	100	1	0
担子菌纲 Basidiomyota
061221	GU256958	Erythrobasidium hasegawianum strain CBS 8253 (AF444522)	617	85.5	1	0
061284	GU256959	Erythrobasidium hasegawianum strain CBS 8253 (AF444522)	635	81.6	1	0
061230	GU256960	Sporobolomyces yunnanensis (AB030353)	601	99.1	10	0
061277	GU256961	Uncultured ectomycorrhiza (Basidiomycota) (AY641466)	701	91.1	4	0
061201	GU256962	Cryptococcus terricola strain CBS 6435 (AF444377)	645	100	2	0
061241	GU256963	Cryptococcus sp. BF73 (AM901689)	631	98.3	1	0
061624	GU256964	Uncultured cf. Tricholoma sp. clone d485.8 (AY254876)	542	79.4	0	14
061265	GU256965	Uncultured cf. Tricholoma sp. clone d485.8 (AY254876)	542	82.4	2	0
061647	GU256966	Uncultured cf. Tricholoma sp. clone d485.8 (AY254876)	536	89.7	0	3
061692	GU256967	Uncultured cf. Tricholoma sp. clone d485.8 (AY254876)	542	80.8	0	1
061632	GU256968	Uncultured cf. Tricholoma (AY097046)	670	98.5	0	2
061628	GU256969	Uncultured ectomycorrhiza (Tricholomataceae) (AM113458)	645	85.4	1	20
061610	GU256970	Tricholoma sp. Umpqua (AF377204)	725	82.6	0	1
061207	GU256971	Uncultured mycorrhiza (Sebacinaceae) 4078 (AY634132)	664	84.6	5	0
061220	GU256972	Uncultured mycorrhiza (Sebacinales) voucher 04A16 (DQ352049)	494	88.5	1	0
0616101	GU256973	Uncultured Sebacinales clone R33 (EF030897)	412	93.0	0	1
061203	GU256974	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	674	92.8	2	20
061209	GU256975	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	674	91.6	2	0
061606	GU256976	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	671	91.6	0	2
061262	GU256977	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	673	90.6	4	0
061607	GU256978	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	689	96.3	0	1
061267	GU256979	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	679	92.1	1	0
061668	GU256980	Uncultured Sebacinales clone Rhoner_6300 (EF127237)	669	91.2	0	2
061675	GU256981	Gymnopus austrosemihirtipes AWW65 (AY263422)	796	95.3	0	4

真菌 Fungus	种类数目 No. of ITS taxa	相对频率 Relative frequency¹
真菌 Fungus	种类数目 No. of ITS taxa	RA	RF
Ascomycota
Helotiales	10	28.2	27.6
Eurotiales	3	16.3	1.9
Oidiodendron	1	9.9	0
Other Ascomycetes	3	5.4	2.8
Subtotal	17	59.8	32.3
Basidiomycota
Sebacinales	10	16.3	24.8
Erythrobasidiales	3	13.0	0
Agaricales	9	7.6	42.9
Filobasidiales	2	3.3	0
Subtotal	24	40.2	67.7

真菌 Fungus	种类数目 No. of ITS taxa	相对频率 Relative frequency¹
真菌 Fungus	种类数目 No. of ITS taxa	RA	RF
Ascomycota
Helotiales	10	28.2	27.6
Eurotiales	3	16.3	1.9
Oidiodendron	1	9.9	0
Other Ascomycetes	3	5.4	2.8
Subtotal	17	59.8	32.3
Basidiomycota
Sebacinales	10	16.3	24.8
Erythrobasidiales	3	13.0	0
Agaricales	9	7.6	42.9
Filobasidiales	2	3.3	0
Subtotal	24	40.2	67.7