Biodiv Sci ›› 2011, Vol. 19 ›› Issue (4): 476-484. DOI: 10.3724/SP.J.1003.2011.09002
Special Issue: 土壤生物与土壤健康
• Original Papers • Previous Articles Next Articles
Fei Luo1,2, Ya Wang2, Qinggui Zeng1, Riming Yan1, Zhibin Zhang1, Du Zhu1,2,*()
Received:
2011-01-12
Accepted:
2011-06-09
Online:
2011-07-20
Published:
2011-07-29
Contact:
Du Zhu
Fei Luo, Ya Wang, Qinggui Zeng, Riming Yan, Zhibin Zhang, Du Zhu. Diversity and plant growth promoting activities of the cultivable rhizobacteria of Dongxiang wild rice (Oryza rufipogon)[J]. Biodiv Sci, 2011, 19(4): 476-484.
Fig. 1 Cultivable rhizobacterial numbers in the three growth stages of Dongxiang wild rice. RS, rice seedling stage; HS, heading stage; GF, grain filling stage. Different small letters indicate significant difference at P < 0. 05.
类群 Microbe groups | 代表菌株 Representative strains | 基因登录号 GenBank accession no. | 最近缘物种(基因登录号) Closest relatives in NCBI (GenBank accession no.) | 最大相似度 Max. identity (%) | 生长阶段(单菌落数) Growing stage (Colony number) |
---|---|---|---|---|---|
变形菌门α亚群 α-Proteobacteria | S109 | GU998805 | Sphingomonas sp. (AB461702) | 99 | HS(21)a; GF(2) |
S22 | HQ647260 | Rhodopseudomonas palustris (AB250613.1) | 99 | HS(1); GF(1) | |
S13 | HQ647259 | Methylobacterium fujisawaense(AB558142) | 100 | RS(2); HS(1); GF(1) | |
变形菌门β亚群 β-Proteobacteria | T26 | HQ443228 | Uncultured Cupriavidus sp. (GQ417899) | 98 | GF(1) |
T24 | HQ647268 | C. basilensis (GU171379.1) | 98 | RS(2); HS(1); GF(2) | |
TS20 | GU998815 | Burkholderia cepacia (FJ652618) | 99 | HS(2); GF(1) | |
变形菌门γ亚群 γ-Proteobacteria | TS4 | GU998802 | Pantoea dispersa (GQ246183) | 99 | RS(4)a; HS(6)a; GF(1) |
T2 | HQ443233 | P. agglomerans (HM130693) | 100 | RS(6)a; HS(6)a; GF(1) | |
TS52 | GU998808 | Uncultured Pseudomonadales bacterium (DQ169126) | 99 | GF(1) | |
TS57 | GU998820 | P. aeruginosa (FJ907193) | 99 | GF(1) | |
P6 | GU998813 | P. poae (FJ937922) | 99 | RS(2); HS(3) | |
F32 | HQ647251 | P. fluorescens (GU198119) | 100 | RS(1) | |
TS51 | GU998810 | Stenotrophomonas maltophilia (GU358076) | 99 | RS(2); HS(1); GF(2) | |
产水菌门 Aquificae | TS54 | HQ647283 | Chryseobacterium indologenes (AY050493) | 98 | GF(2) |
厚壁菌门 Firmicutes | S71 | GU998803 | Bacillus subtilis (FJ392727) | 99 | RS(6)a; HS(5)a; GF(4)a |
T184 | HQ647278 | B. megaterium (EU594558) | 99 | RS(3); HS(2); GF(5)a | |
T47 | GU998812 | B. koreensis (AY667496) | 100 | GF(3)a | |
S59 | HQ443223 | B. aryabhattai (GU563348) | 99 | HS(1); GF(2) | |
T111 | HQ443236 | B. cereus (HM752769) | 98 | RS(1); HS(1); GF(3)a | |
T172 | HQ647277 | B. pumilus (FJ763642) | 99 | RS(2); HS(1); GF(2) |
Table 1 Cultivable bacteria isolates from rhizosphere soil of Dongxiang wild rice
类群 Microbe groups | 代表菌株 Representative strains | 基因登录号 GenBank accession no. | 最近缘物种(基因登录号) Closest relatives in NCBI (GenBank accession no.) | 最大相似度 Max. identity (%) | 生长阶段(单菌落数) Growing stage (Colony number) |
---|---|---|---|---|---|
变形菌门α亚群 α-Proteobacteria | S109 | GU998805 | Sphingomonas sp. (AB461702) | 99 | HS(21)a; GF(2) |
S22 | HQ647260 | Rhodopseudomonas palustris (AB250613.1) | 99 | HS(1); GF(1) | |
S13 | HQ647259 | Methylobacterium fujisawaense(AB558142) | 100 | RS(2); HS(1); GF(1) | |
变形菌门β亚群 β-Proteobacteria | T26 | HQ443228 | Uncultured Cupriavidus sp. (GQ417899) | 98 | GF(1) |
T24 | HQ647268 | C. basilensis (GU171379.1) | 98 | RS(2); HS(1); GF(2) | |
TS20 | GU998815 | Burkholderia cepacia (FJ652618) | 99 | HS(2); GF(1) | |
变形菌门γ亚群 γ-Proteobacteria | TS4 | GU998802 | Pantoea dispersa (GQ246183) | 99 | RS(4)a; HS(6)a; GF(1) |
T2 | HQ443233 | P. agglomerans (HM130693) | 100 | RS(6)a; HS(6)a; GF(1) | |
TS52 | GU998808 | Uncultured Pseudomonadales bacterium (DQ169126) | 99 | GF(1) | |
TS57 | GU998820 | P. aeruginosa (FJ907193) | 99 | GF(1) | |
P6 | GU998813 | P. poae (FJ937922) | 99 | RS(2); HS(3) | |
F32 | HQ647251 | P. fluorescens (GU198119) | 100 | RS(1) | |
TS51 | GU998810 | Stenotrophomonas maltophilia (GU358076) | 99 | RS(2); HS(1); GF(2) | |
产水菌门 Aquificae | TS54 | HQ647283 | Chryseobacterium indologenes (AY050493) | 98 | GF(2) |
厚壁菌门 Firmicutes | S71 | GU998803 | Bacillus subtilis (FJ392727) | 99 | RS(6)a; HS(5)a; GF(4)a |
T184 | HQ647278 | B. megaterium (EU594558) | 99 | RS(3); HS(2); GF(5)a | |
T47 | GU998812 | B. koreensis (AY667496) | 100 | GF(3)a | |
S59 | HQ443223 | B. aryabhattai (GU563348) | 99 | HS(1); GF(2) | |
T111 | HQ443236 | B. cereus (HM752769) | 98 | RS(1); HS(1); GF(3)a | |
T172 | HQ647277 | B. pumilus (FJ763642) | 99 | RS(2); HS(1); GF(2) |
Fig. 2 Neighbor-joining tree based on 16S rRNA gene sequences analysis showing the phylogenetic relationships among the strains and their closely related taxa. Bootstrap values (percent) calculated from 1,000 resamplings are shown at branch nodes. GenBank accession numbers are given in the parentheses.
生长阶段 Growth stages | 多样性 指数 H | 均匀度 指数 J | 优势度 指数 D | 丰富度 指数 S |
---|---|---|---|---|
秧苗期 RS | 2.232 | 0.931 | 0.097 | 11 |
抽穗期 HS | 2.037 | 0.772 | 0.208 | 14 |
成熟期 GF | 2.741 | 0.948 | 0.074 | 18 |
Table 2 The diversity index (H), richness (S), evenness index (J) and dominance index (D) of cultivable rhizobacteria from Dongxiang wild rice
生长阶段 Growth stages | 多样性 指数 H | 均匀度 指数 J | 优势度 指数 D | 丰富度 指数 S |
---|---|---|---|---|
秧苗期 RS | 2.232 | 0.931 | 0.097 | 11 |
抽穗期 HS | 2.037 | 0.772 | 0.208 | 14 |
成熟期 GF | 2.741 | 0.948 | 0.074 | 18 |
Table 3 Number and PGPA of cultivable PGPR's isolated from rhizospheric soil of Dongxiang wild rice in the three growth stages. Number in parentheses represents number of isolates.
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