Biodiv Sci ›› 2008, Vol. 16 ›› Issue (1): 91-95.  DOI: 10.1360/biodiv.070239

Special Issue: 土壤生物与土壤健康

• Original article • Previous Articles     Next Articles

Culturable bacterial diversity in rhizosphere ofBashania fangiana

Min Liu1,2, Lubin Li2, Kai Yang3, Jigang Han1, Baocheng Zhu1,4,*(), Zhenhua Peng2   

  1. 1 College of Life Science, Hebei University, Baoding 071002
    2 Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091
    3 Key Laboratory of New Technology in Agricultural Application in Beijing, Beijing Agricultural College, Beijing 102206
    4 College of Life Science, Agricultural University of Hebei, Baoding 071001
  • Received:2007-09-03 Accepted:2007-11-15 Online:2008-01-27 Published:2008-01-27
  • Contact: Baocheng Zhu


In order to understand the diversity of culturable bacteria, we collected Bashania fangiana rhizosphere soil in Wolong Nature Reserve in May 2006. Then we isolated and cultured 50 bacteria strains with different morphological character of colonies from the soil. Phylogenetic analysis based on the partial 16S rDNA sequences indicated that the 50 strains were clustered into ten genera: 27 strains of Gammaproteobacteria (42.3%), nine strains of Firmicutes (26.9%), four strains of Actinobacteria (15.4%), six strains of Betaproteobacteria (7.7%), three strains of Alphaproteobacteria (3.9%), one strain closely related to the genus Pedobacter.The dominant bacteria were Pseudomonasand Bacillus. Additionally, we found two potential novel species. The result shows that the bacterial population diversity is abundant in B. fangiana rhizosphere soil.

Key words: Bashania fangiana, rhizosphere bacteria, culturable bacteria, diversity