Biodiv Sci ›› 2010, Vol. 18 ›› Issue (4): 414-419. DOI: 10.3724/SP.J.1003.2010.414
Special Issue: 传粉生物学; 昆虫多样性与生态功能
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Yiyi Long, Liyuan Yang, Wanjin Liao()
Received:
2010-04-22
Accepted:
2010-06-26
Online:
2010-07-20
Published:
2010-07-20
Contact:
Wanjin Liao
Yiyi Long, Liyuan Yang, Wanjin Liao. Identifying cryptic species in pollinating-fig wasps by PCR-RFLP on mtDNA COI gene[J]. Biodiv Sci, 2010, 18(4): 414-419.
样品编号 Sample No. | 大果榕小蜂 C. emarginatus | 鸡嗉果榕小蜂 C. gravelyi | |||
---|---|---|---|---|---|
隐种A头数 Number of A | 隐种B头数Number of B | 隐种A头数 Number of A | 隐种B头数 Number of B | ||
1 | 1 | 10 | 6 | 2 | |
2 | 6 | 2 | 10 | 2 | |
3 | 10 | 2 | 20 | 2 | |
4 | 20 | 2 | 1 | 10 | |
5 | 2 | 20 | 2 | 20 | |
6 | 2 | 10 | 2 | 10 | |
7 | 2 | 6 | 2 | 6 | |
8 | 4 | 4 | 4 | 4 | |
9 | 0 | 2 | 0 | 2 | |
10 | 2 | 0 | 2 | 0 |
Table 1 Composition of cryptic species (A and B) in Ceratosolen emarginatus and C. gravelyi used in this study
样品编号 Sample No. | 大果榕小蜂 C. emarginatus | 鸡嗉果榕小蜂 C. gravelyi | |||
---|---|---|---|---|---|
隐种A头数 Number of A | 隐种B头数Number of B | 隐种A头数 Number of A | 隐种B头数 Number of B | ||
1 | 1 | 10 | 6 | 2 | |
2 | 6 | 2 | 10 | 2 | |
3 | 10 | 2 | 20 | 2 | |
4 | 20 | 2 | 1 | 10 | |
5 | 2 | 20 | 2 | 20 | |
6 | 2 | 10 | 2 | 10 | |
7 | 2 | 6 | 2 | 6 | |
8 | 4 | 4 | 4 | 4 | |
9 | 0 | 2 | 0 | 2 | |
10 | 2 | 0 | 2 | 0 |
Fig. 1 The PCR products of COI gene in Ceratosolen emarginatus (A) and C. gravelyi (B). M indicates the DNA marker, and the numbers indicate the sample number. See Table 1 for the composition of cryptic species for each sample.
Fig. 2 The digested products by XhoI (a and b) and BssSI (c and d) in Ceratosolen emarginatus. a and c show the digested results of purified PCR products; b and d, unpurified. M is the DNA marker (DL 2000). 1-10 correspond to the sample numbers in Table 1.
Fig. 3 The digested products by BmrI (a and b) and AvaI (c and d) in Ceratosolen gravelyi. a and c show the digested results of purified PCR products; b and d, unpurified. 1-10 correspond to the sample numbers in Table 1.
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