Biodiv Sci ›› 2015, Vol. 23 ›› Issue (1): 50-60.DOI: 10.17520/biods.2014089

• Original Papers: Animal Diversity • Previous Articles     Next Articles

DNA barcoding of hydromedusae in northern Beibu Gulf for species identification

Dangni Zhang3, Lianming Zheng1,2,3,*(), Jinru He3, Wenjing Zhang1,3, Yuanshao Lin1,3, Yang Li3   

  1. 1 Marine Biodiversity and Global Change Research Center, Xiamen University, Xiamen, Fujian 361102
    2 Fujian Provincial Key Laboratory for Coastal Ecology and Environmental Studies (CEES), Xiamen University, Xiamen, Fujian 361102
    3 College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian 361102;
  • Received:2014-05-09 Accepted:2014-11-19 Online:2015-01-20 Published:2015-05-04
  • Contact: Lianming Zheng

Abstract:

Being a major component of coastal zooplankton assemblages, hydromedusae play a key role in material recycling and energy flow of marine ecosystems. Species identification is challenging for this group due to their phonetic simplicity. DNA barcoding provides an efficient method for species identification. In the present study, 92 COI and 116 16S sequences from 28 hydromedusae species were amplified. A neighbor- joining phylogenetic tree was constructed based on Kimura 2-parameter genetic distance and further studied using Klee-diagram vector analysis. Intra-specific K2P genetic distance averaged 0.008±0.005 (0-0.033) for COI, and 0.006±0.010 (0-0.047) for 16S; inter-specific K2P genetic distance averaged 0.298±0.128 (0.092- 0.597), and 0.394±0.195 (0.068-0.898) for COI and 16S, respectively. An obvious “barcoding gap” was detected for all species in both markers and all individuals of a species clustered together in both the COI and 16S trees. Further confirmatory evidence was also provided through indicator vector analysis. Hence, both COI and 16S appear to be accurate and efficient markers for hydromedusae identification in northern Beibu Gulf.

Key words: DNA barcoding, molecular marker, vector analysis, species identification, hydromedusae