Genetic diversity in rhizobia isolated from Sphaerophysa salsula in several regions of northwestern China

doi:10.3724/SP.J.1003.2009.08218

Abstract

Abstract:

Sphaerophysa salsula is a highly nutritive and drought-tolerant perennial grass distributed in mid-Asia and northwestern China. This legume plant is highly prized for the revegetation of drought and high alkali soils. Our objective was to better understand the diversity and phylogeny of rhizobia collected from nodules of Sphaerophsa salsula in different regions of northwestern China. We estimated genetic diversity using 16S rDNA PCR-RFLP and 16S rDNA sequencing. Nine genotypes were tested from 57 strains. 16S rDNA sequencing revealed that these rhizobial strains belonged to the following genera: Mesorhizobium, Rhizobium, Sinorhizobium, Agrobacterium, Phyllobacterium and Shinella kummerowiae. The Jaccard similarity coefficient of the strains from Yinchuan was low while the strains isolated from Minle and Linze had rich diversity, with Simpson indices of 0.826 and 0.710, respectively, and Shannon-Wiener indices of 1.831 and 1.530, respectively. This study provides basic information for the phylogeny of rhizobia in northwestern China.

Key words: Sphaerophysa salsula, phylogeny, 16S rDNA PCR-RFLP, 16S rDNA sequencing, genetic diversity

Lin Xu, Jiajie Xu, Qiaoli Liu, Ruimei Xie, Gehong Wei. Genetic diversity in rhizobia isolated from Sphaerophysa salsula in several regions of northwestern China[J]. Biodiv Sci, 2009, 17(1): 69-75.

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URL: https://www.biodiversity-science.net/EN/10.3724/SP.J.1003.2009.08218

https://www.biodiversity-science.net/EN/Y2009/V17/I1/69

Figures/Tables 4

References 22

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酶切类型 Fingerprint pattern	分离地 Location
酶切类型 Fingerprint pattern	甘肃民乐县 Minle County, Gansu	甘肃张掖市 Zhangye City, Gansu	甘肃临泽县 Linze County, Gansu	甘肃玉门市 Yumen City, Gansu	宁夏银川市 Yinchuan City, Ningxia	新疆伊犁市 Yili City, Xinjiang
AAB	GS0181, GS0185, GS0188, GS0192, GS0198	GS0215	GS0240
BBD	GS0184		GS0218	GS0237
CCB	GS0170, GS0171 GS0168		GS0219, GS0223, GS0227, GS0230, GS0231, GS0232		NX0171, NX0172, NX0173	XJ0216
CEB	GS0176, GS0196, GS0199, GS0200, GS0201, GS0204	GS0206, GS0209 GS0210, GS0214	GS0216, GS0233 GS0234
DDB			GS0228
DFB	GS0172, GS0202
EDB	GS0177, GS0178, GS0187			GS0238
EDC	GS0166, GS0169, GS0173, GS0180	GS0207, GS0211, GS0212, GS0213	GS0220			XJ0217, XJ0218, XJ0219, XJ0221
FDC						XJ0229

酶切类型 Fingerprint pattern	分离地 Location
酶切类型 Fingerprint pattern	甘肃民乐县 Minle County, Gansu	甘肃张掖市 Zhangye City, Gansu	甘肃临泽县 Linze County, Gansu	甘肃玉门市 Yumen City, Gansu	宁夏银川市 Yinchuan City, Ningxia	新疆伊犁市 Yili City, Xinjiang
AAB	GS0181, GS0185, GS0188, GS0192, GS0198	GS0215	GS0240
BBD	GS0184		GS0218	GS0237
CCB	GS0170, GS0171 GS0168		GS0219, GS0223, GS0227, GS0230, GS0231, GS0232		NX0171, NX0172, NX0173	XJ0216
CEB	GS0176, GS0196, GS0199, GS0200, GS0201, GS0204	GS0206, GS0209 GS0210, GS0214	GS0216, GS0233 GS0234
DDB			GS0228
DFB	GS0172, GS0202
EDB	GS0177, GS0178, GS0187			GS0238
EDC	GS0166, GS0169, GS0173, GS0180	GS0207, GS0211, GS0212, GS0213	GS0220			XJ0217, XJ0218, XJ0219, XJ0221
FDC						XJ0229

来源 Location	Jaccard相似性系数 Jaccard similarity coefficient					Simpson 指数 Simpson index	Shannon-Wiener指数 Shannon-Wiener index
来源 Location	甘肃张掖市 Zhangye City, Gansu	甘肃临泽县 Linze County, Gansu	甘肃玉门市 Yumen City, Gansu	宁夏银川市 Yinchuan City, Ningxia	新疆伊犁市 Yili City, Xinjiang	Simpson 指数 Simpson index	Shannon-Wiener指数 Shannon-Wiener index
甘肃张掖市 Zhangye City, Gansu						0.593	0. 965
甘肃临泽县 Linze County, Gansu	0.50					0.710	1.530
甘肃玉门市 Yumen City, Gansu	0.25	0.14				0.500	0.693
宁夏银川市 Yinchuan City, Ningxia	0.00	0.17	0.00			0.000	0.000
新疆伊犁市 Yili City, Xinjiang	0.20	0.30	0.00	0.33		0.500	0.868
甘肃民乐县 Minle County, Gansu	0.38	0.56	0.25	0.13	0.22	0.826	1.831

来源 Location	Jaccard相似性系数 Jaccard similarity coefficient					Simpson 指数 Simpson index	Shannon-Wiener指数 Shannon-Wiener index
来源 Location	甘肃张掖市 Zhangye City, Gansu	甘肃临泽县 Linze County, Gansu	甘肃玉门市 Yumen City, Gansu	宁夏银川市 Yinchuan City, Ningxia	新疆伊犁市 Yili City, Xinjiang	Simpson 指数 Simpson index	Shannon-Wiener指数 Shannon-Wiener index
甘肃张掖市 Zhangye City, Gansu						0.593	0. 965
甘肃临泽县 Linze County, Gansu	0.50					0.710	1.530
甘肃玉门市 Yumen City, Gansu	0.25	0.14				0.500	0.693
宁夏银川市 Yinchuan City, Ningxia	0.00	0.17	0.00			0.000	0.000
新疆伊犁市 Yili City, Xinjiang	0.20	0.30	0.00	0.33		0.500	0.868
甘肃民乐县 Minle County, Gansu	0.38	0.56	0.25	0.13	0.22	0.826	1.831