Biodiv Sci ›› 2009, Vol. 17 ›› Issue (1): 69-75. DOI: 10.3724/SP.J.1003.2009.08218
• Paper • Previous Articles Next Articles
Lin Xu1,2, Jiajie Xu1, Qiaoli Liu1, Ruimei Xie1, Gehong Wei1,*()
Received:
2008-08-28
Accepted:
2008-12-14
Online:
2009-01-20
Published:
2009-01-20
Contact:
Gehong Wei
Lin Xu, Jiajie Xu, Qiaoli Liu, Ruimei Xie, Gehong Wei. Genetic diversity in rhizobia isolated from Sphaerophysa salsula in several regions of northwestern China[J]. Biodiv Sci, 2009, 17(1): 69-75.
酶切类型 Fingerprint pattern | 分离地 Location | |||||
---|---|---|---|---|---|---|
甘肃民乐县 Minle County, Gansu | 甘肃张掖市 Zhangye City, Gansu | 甘肃临泽县 Linze County, Gansu | 甘肃玉门市 Yumen City, Gansu | 宁夏银川市 Yinchuan City, Ningxia | 新疆伊犁市 Yili City, Xinjiang | |
AAB | GS0181, GS0185, GS0188, GS0192, GS0198 | GS0215 | GS0240 | |||
BBD | GS0184 | GS0218 | GS0237 | |||
CCB | GS0170, GS0171 GS0168 | GS0219, GS0223, GS0227, GS0230, GS0231, GS0232 | NX0171, NX0172, NX0173 | XJ0216 | ||
CEB | GS0176, GS0196, GS0199, GS0200, GS0201, GS0204 | GS0206, GS0209 GS0210, GS0214 | GS0216, GS0233 GS0234 | |||
DDB | GS0228 | |||||
DFB | GS0172, GS0202 | |||||
EDB | GS0177, GS0178, GS0187 | GS0238 | ||||
EDC | GS0166, GS0169, GS0173, GS0180 | GS0207, GS0211, GS0212, GS0213 | GS0220 | XJ0217, XJ0218, XJ0219, XJ0221 | ||
FDC | XJ0229 |
Table 1 16S rDNA PCR-RFLP fingerprint patterns of the rhizobia collected from Sphaerophsa salsula nodules in different regions of northwestern China. The boldfaces are strains for 16S rDNA sequencing.
酶切类型 Fingerprint pattern | 分离地 Location | |||||
---|---|---|---|---|---|---|
甘肃民乐县 Minle County, Gansu | 甘肃张掖市 Zhangye City, Gansu | 甘肃临泽县 Linze County, Gansu | 甘肃玉门市 Yumen City, Gansu | 宁夏银川市 Yinchuan City, Ningxia | 新疆伊犁市 Yili City, Xinjiang | |
AAB | GS0181, GS0185, GS0188, GS0192, GS0198 | GS0215 | GS0240 | |||
BBD | GS0184 | GS0218 | GS0237 | |||
CCB | GS0170, GS0171 GS0168 | GS0219, GS0223, GS0227, GS0230, GS0231, GS0232 | NX0171, NX0172, NX0173 | XJ0216 | ||
CEB | GS0176, GS0196, GS0199, GS0200, GS0201, GS0204 | GS0206, GS0209 GS0210, GS0214 | GS0216, GS0233 GS0234 | |||
DDB | GS0228 | |||||
DFB | GS0172, GS0202 | |||||
EDB | GS0177, GS0178, GS0187 | GS0238 | ||||
EDC | GS0166, GS0169, GS0173, GS0180 | GS0207, GS0211, GS0212, GS0213 | GS0220 | XJ0217, XJ0218, XJ0219, XJ0221 | ||
FDC | XJ0229 |
Fig. 2 A phylogenetic tree based on 16S rDNA sequences using neighbour-joining method. The figures on the branches indicate the reliabilities. GenBank accession numbers are in the parenthesis. The boldfaces are strains for 16S rDNA sequencing.
来源 Location | Jaccard相似性系数 Jaccard similarity coefficient | Simpson 指数 Simpson index | Shannon-Wiener指数 Shannon-Wiener index | ||||
---|---|---|---|---|---|---|---|
甘肃张掖市 Zhangye City, Gansu | 甘肃临泽县 Linze County, Gansu | 甘肃玉门市 Yumen City, Gansu | 宁夏银川市 Yinchuan City, Ningxia | 新疆伊犁市 Yili City, Xinjiang | |||
甘肃张掖市 Zhangye City, Gansu | 0.593 | 0. 965 | |||||
甘肃临泽县 Linze County, Gansu | 0.50 | 0.710 | 1.530 | ||||
甘肃玉门市 Yumen City, Gansu | 0.25 | 0.14 | 0.500 | 0.693 | |||
宁夏银川市 Yinchuan City, Ningxia | 0.00 | 0.17 | 0.00 | 0.000 | 0.000 | ||
新疆伊犁市 Yili City, Xinjiang | 0.20 | 0.30 | 0.00 | 0.33 | 0.500 | 0.868 | |
甘肃民乐县 Minle County, Gansu | 0.38 | 0.56 | 0.25 | 0.13 | 0.22 | 0.826 | 1.831 |
Table 2 The Jaccard similarity coefficient and diversity indices of Sphaerophysa salsula rhizobia isolated from different regions of northwestern China
来源 Location | Jaccard相似性系数 Jaccard similarity coefficient | Simpson 指数 Simpson index | Shannon-Wiener指数 Shannon-Wiener index | ||||
---|---|---|---|---|---|---|---|
甘肃张掖市 Zhangye City, Gansu | 甘肃临泽县 Linze County, Gansu | 甘肃玉门市 Yumen City, Gansu | 宁夏银川市 Yinchuan City, Ningxia | 新疆伊犁市 Yili City, Xinjiang | |||
甘肃张掖市 Zhangye City, Gansu | 0.593 | 0. 965 | |||||
甘肃临泽县 Linze County, Gansu | 0.50 | 0.710 | 1.530 | ||||
甘肃玉门市 Yumen City, Gansu | 0.25 | 0.14 | 0.500 | 0.693 | |||
宁夏银川市 Yinchuan City, Ningxia | 0.00 | 0.17 | 0.00 | 0.000 | 0.000 | ||
新疆伊犁市 Yili City, Xinjiang | 0.20 | 0.30 | 0.00 | 0.33 | 0.500 | 0.868 | |
甘肃民乐县 Minle County, Gansu | 0.38 | 0.56 | 0.25 | 0.13 | 0.22 | 0.826 | 1.831 |
[1] | Beringer JE (1974) R-factor transfer in Rhizobium leguminosarum. Journal of General Microbiology, 84, 188-198. |
[2] | Chen WX (陈文新), Wang ET (汪恩涛), Chen WF (陈文峰) (2004) The relationship between the symbiotic promiscuity of rhizobia and legumes and their geographical environments. Scientia Agricultura Sinica (中国农业科学), 37, 81-86. (in Chinese with English abstract) |
[3] | Gao LF (高丽锋), Deng X (邓馨), Wang HX (王洪新) Hu ZA (胡志昂) (2004) Diversity and resistance of rhizobia isolated from Caragana intermedia in Maowusu sandland. Chinese Journal of Applied Ecology (应用生态学报), 15, 44-48. (in Chinese with English abstract) |
[4] | Gaunt MW, Turner SL, Rigottier GL, Rigottier-Gois L, Lloyd-Macgilp SA, Young JPW (2001) Phylogenies of atpD and recA support the small subunit rRNA-based classification of rhizobia. International Journal of Systematic and Evolutionary Microbiology, 51, 2037-2048. |
[5] | Ma KP (马克平) (1994) The measurement of community diversity. In: Principles and Methodologies of Biodiversity Studies (生物多样性研究的原理与方法), pp. 141-165, China Science and Technology Press, Beijing. |
[6] |
Mhamdi R, Mrabet M, Laguerre G, Tiwari R, Aouani ME (2005) Colonization of Phaseolus vulgaris nodules by Agrobacterium-like strains. Canadian Journal of Microbiology, 51, 105-111.
URL PMID |
[7] | Martens M, Dawyndt P, Coopman R, Gillis M, De Vos P, Willems A (2008) Advantages of multilocus sequence analysis for taxonomic studies: a case study using 10 housekeeping genes in the genus Ensifer (including former Sinorhizobium). International Journal of Systematic and Evolutionary Microbiology, 58, 200-214. |
[8] | Peng GX, Tan ZY, Wang ET, Reinhold-Hurek B, Chen WF and Chen WX (2002) Identification of isolates from soybean nodules in Xinjiang region as Sinorhizobium xinjiangense and genetic differentiation of S. xinjiangense from Sinorhizobium fredii. International Journal of Systematic and Evolutionary Microbiology, 52, 457-462. |
[9] | Selenska-Pobell S, Evguenieva-Hackenberg E, Radeva G, Squartini A (1996) Characterization of Rhizobium‘hedysari’by RFLP analysis of PCR amplified rDNA and by genomic PCR fingerprinting. Journal of Applied Bacteriology, 80, 517-528. |
[10] |
Stackebrandt E, Frederiksen W, Garrity GM, Grimont PAD, Kampfer P, Maiden MCJ, Nesme X, Rossello-Mora R, Swings J, Truper HG, Vauterin L, Ward AC, Whitman WB (2002) Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. International Journal of Systematic and Evolutionary Microbiology, 52, 1043-1047.
URL PMID |
[11] | Tan ZY (谭志远), Chen WX (陈文新) (1997) Sequencing the 16S rDNA of representative strain of new rhizobial group and determining of its phylogenetic relationship. Acta Microbiologica Sinica (微生物学报), 37, 411-416. (in Chinese with English abstract) |
[12] |
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876-4882.
URL PMID |
[13] | van de Peer Y, De Wachter R (1994) TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Computer Applications in the Bioscience, 10, 569-570. |
[14] | Vandamme P, Pot B, Gillis M, de Vos P, Kersters K, Swings J (1996) Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiological Reviews, 60, 407-438. |
[15] | Vincent JM (1970) A Manual for the Practical Study of Rroot-Nodule Bacteria. IBP Handbook 15. Blackwell Scientific Publications, Oxford. |
[16] | Wang LL, Wang ET, Liu J, Li Y, Chen WX (2006) Endophytic occupation of root nodules and roots of Melilotus dentatus by Agrobacterium tumefaciens. Microbial Ecology, 52, 436-443. |
[17] | Wu D (吴达), Shi YP (师彦平), Liang B (梁冰), Wang JH (王建华) (2003) Advances in studies on swainsonine. Chinese Traditional and Herbal Drugs (中草药), 34, A5-7. (in Chinese) |
[18] | Xu L (徐莉), Yang JK (杨江科), Zhang WT (张伟涛), Yuan TY (袁天英), Zhou JC (周俊初) (2007) Phenotypic and genetic diversity of cowpea rhizobia. Acta Ecologica Sinina (生态学报), 27, 3655-3662. (in Chinese with English abstract) |
[19] | Yang J, Hu Z, Guo GQ, Zheng GC (2001) In vitro plant regeneration from cotyledon explants of Swainsona salsula Taubert. Plant Cell, Tissue and Organ Culture, 66, 35-39. |
[20] | Yao RC (姚荣成) (2003) Advances in studies on Swainsona salsula. The Journal of Pharmaceutical Practice (药学实践杂志), 21, 43-44. (in Chinese) |
[21] | Zhang Y (张英) (1999) The nutrition and feed value of Sphaerophysa salsula Tauberts. Feed Research (饲料研究), (7), 22. (in Chinese) |
[22] | Zou L (邹林), Yang MQ (杨鸣琦) (2006) Progress on anti-tumor activity of swainsonine. Progress in Veterinary Medicine (动物医学进展), 27(4), 36-38. (in Chinese with English abstract) |
[1] | Weiyue Sun, Jiangping Shu, Yufeng Gu, Morigengaowa, Xiajin Du, Baodong Liu, Yuehong Yan. Conservation genomics analysis revealed the endangered mechanism of Adiantum nelumboides [J]. Biodiv Sci, 2022, 30(7): 21508-. |
[2] | Ting Wang, Jiangping Shu, Yufeng Gu, Yanqing Li, Tuo Yang, Zhoufeng Xu, Jianying Xiang, Xianchun Zhang, Yuehong Yan. Insight into the studies on diversity of lycophytes and ferns in China [J]. Biodiv Sci, 2022, 30(7): 22381-. |
[3] | Jianming Wang, Mengjun Qu, Yin Wang, Yiming Feng, Bo Wu, Qi Lu, Nianpeng He, Jingwen Li. The drivers of plant taxonomic, functional, and phylogenetic β-diversity in the gobi desert of northern Qinghai-Tibet Plateau [J]. Biodiv Sci, 2022, 30(6): 21503-. |
[4] | Togtokh Mongke, Dongyi Bai, Tugeqin Bao, Ruoyang Zhao, Tana An, Aertengqimike Tiemuqier, Baoyindeligeer Mongkejargal, Has Soyoltiin, Manglai Dugarjaviin, Haige Han. Assessment of SNPs-based genomic diversity in different populations of Eastern Asian landrace horses [J]. Biodiv Sci, 2022, 30(5): 21031-. |
[5] | Jing Cui, Mingfang Xu, Qun Zhang, Yao Li, Xiaoshu Zeng, Sha Li. Differences in genetic diversity of Pleuronichthys cornutus in the coastal water of China and Japan based on three mitochondrial markers [J]. Biodiv Sci, 2022, 30(5): 21485-. |
[6] | Xiaoyan Jiang, Shengjie Gao, Yan Jiang, Yun Tian, Xin Jia, Tianshan Zha. Species diversity, functional diversity, and phylogenetic diversity in plant communities at different phases of vegetation restoration in the Mu Us sandy grassland [J]. Biodiv Sci, 2022, 30(5): 21387-. |
[7] | Xinyu Cai, Xiaowei Mao, Yiqiang Zhao. Methods and research progress on the origin of animal domestication [J]. Biodiv Sci, 2022, 30(4): 21457-. |
[8] | Jun Sun, Yuyao Song, Yifeng Shi, Jian Zhai, Wenzhuo Yan. Progress of marine biodiversity studies in China seas in the past decade [J]. Biodiv Sci, 2022, 30(10): 22526-. |
[9] | Dongmei Li, Weihong Yang, Qingduo Li, Xi Han, Xiuping Song, Hong Pan, Yun Feng. High prevalence and genetic variation of Bartonella species inhabiting the bats in southwestern Yunnan [J]. Biodiv Sci, 2021, 29(9): 1245-1255. |
[10] | Yangkang Chen, Yi Wang, Jialiang Li, Wentao Wang, Duanyu Feng, Kangshan Mao. Principles, error sources and application suggestions of prevailing molecular dating methods [J]. Biodiv Sci, 2021, 29(5): 629-646. |
[11] | Bo Chen, Lan Jiang, Ziyang Xie, Yangdi Li, Jiaxuan Li, Mengjia Li, Chensi Wei, Cong Xing, Jinfu Liu, Zhongsheng He. Taxonomic and phylogenetic diversity of plants in a Castanopsis kawakamiinatural forest [J]. Biodiv Sci, 2021, 29(4): 439-448. |
[12] | Zhi Yao, Jun Guo, Chenzhong Jin, Yongbo Liu. Endangered mechanisms for the first-class protected Wild Plants with Extremely Small Populations in China [J]. Biodiv Sci, 2021, 29(3): 394-408. |
[13] | Junwei Ye, Bin Tian. Genetic structure and its causes of an important woody oil plant in Southwest China, Prinsepia utilis (Rosaceae) [J]. Biodiv Sci, 2021, 29(12): 1629-1637. |
[14] | Denggao Xiang, Yuefei Li, Xinhui Li, Weitao Chen, Xiuhui Ma. Population structure and genetic diversity of Culter recurviceps revealed by multi-loci [J]. Biodiv Sci, 2021, 29(11): 1505-1512. |
[15] | Jinyuan Su, Yu Yan, Chong Li, Dan Li, Fang K. Du. Informing conservation strategies with genetic diversity in Wild Plant with Extremely Small Populations: A review on gymnosperms [J]. Biodiv Sci, 2020, 28(3): 376-384. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||
Copyright © 2022 Biodiversity Science
Editorial Office of Biodiversity Science, 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: 010-62836137, 62836665 E-mail: biodiversity@ibcas.ac.cn