Monophyletic origin and synonymic phenomena in the sub-family Cynoglossinae inferred from mitochondrial DNA sequences

doi:10.3724/SP.J.1003.2010.275

Abstract

Abstract:

Morphological taxonomy in the fish sub-family Cynoglossinae is complicated and contentious. To clarify these ambiguities, the molecular phylogenic relationships among 14 Cynoglossinae species were studied by analyzing partial 16S rRNA and Cyt b mitochondrial gene sequences. Similar phylogentic topology inferred from both gene fragments demonstrated that Cynoglossinae is a monophyletic group. However, the phylogenetic relationships revealed by the mitochondrial DNA sequence analyses were not consistent with those inferred from morphological classification. In contrast with morphological classification, reciprocal monophyly was not detected between Paraplagusis japonica and the species in the genus Cynoglossus. Neighbor-Joining (NJ) and Maximum Parsimony (MP) trees showed that those species in the three sub-genera Cynoglossus, Cynoglossoides and Areliscus clustered on an independent branch. Also, synonymic phenomena possibly existed in the following three groups of species: C. lighti andC. joyner, C. abbreviatus and C. purpureomaculatus, as well as in C. semilaevis, C. gracilis and C. trigrammus. Our results highlight the open questions surrounding the morphological classification of Cynoglossinae species. Information on molecular phylogenic relationships will likely be of help in revising the morphological classification of Cynoglossinae species.

Key words: Cynoglossinae, 16S rRNA, Cyt b, molecular phylogenic relationship

Shufang Liu, Jinxian Liu, Zhimeng Zhuang, Tianxiang Gao, Zhiqiang Han, Dagang Chen. Monophyletic origin and synonymic phenomena in the sub-family Cynoglossinae inferred from mitochondrial DNA sequences[J]. Biodiv Sci, 2010, 18(3): 275-282.

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URL: https://www.biodiversity-science.net/EN/10.3724/SP.J.1003.2010.275

https://www.biodiversity-science.net/EN/Y2010/V18/I3/275

Figures/Tables 4

References 22

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种名 Species	亚属名 Sub-genus	样本量 Sample size	Cytb测定数 No. of Cytb DNA	16S rRNA测定数 No. of 16S rRNA
内群 Ingroup
1 双线舌鳎 Cynoglossus bilineatus	Arelia	2	2	1
2 中华舌鳎 C. sinicus	Cynoglossus	2	1	1
3 褐斑三线舌鳎 C. trigrammus	Areliscus	1	1	1
4 窄体舌鳎 C. gracilis	Areliscus	2	1	1
5 长吻红舌鳎 C. lighti	Areliscus	2	2	1
6 短吻红舌鳎 C. joyneri	Areliscus	2	2	1
7 黑鳃舌鳎 C. roulei	Areliscus	1	1	1
8 紫斑舌鳎 C. purpureomaculatus	Areliscus	2	1	1
9 短吻三线舌鳎 C. abbreviatus	Areliscus	5	2	2
10 半滑舌鳎 C. semilaevis	Areliscus	3	2	1
11 斑头舌鳎 C. puncticeps	Cynoglossoides	1	1	1
12 少鳞舌鳎 C. oligolepis	Cynoglossoides	2	2	1
13 宽体舌鳎 C. robustus	Cynoglossoides	2	1	1
14 日本须鳎 Paraplagusis japonica	Rhinoplagusia	1	1	1
外群 Outgroup
15 塞内加尔鳎 Sole senegalensis		3	3	2
16 人字钩嘴鳎 Heteromycteris matsubarai		2	1	2
17 圆斑星鲽 Verasper variegatus		1	1	1

种名 Species	亚属名 Sub-genus	样本量 Sample size	Cytb测定数 No. of Cytb DNA	16S rRNA测定数 No. of 16S rRNA
内群 Ingroup
1 双线舌鳎 Cynoglossus bilineatus	Arelia	2	2	1
2 中华舌鳎 C. sinicus	Cynoglossus	2	1	1
3 褐斑三线舌鳎 C. trigrammus	Areliscus	1	1	1
4 窄体舌鳎 C. gracilis	Areliscus	2	1	1
5 长吻红舌鳎 C. lighti	Areliscus	2	2	1
6 短吻红舌鳎 C. joyneri	Areliscus	2	2	1
7 黑鳃舌鳎 C. roulei	Areliscus	1	1	1
8 紫斑舌鳎 C. purpureomaculatus	Areliscus	2	1	1
9 短吻三线舌鳎 C. abbreviatus	Areliscus	5	2	2
10 半滑舌鳎 C. semilaevis	Areliscus	3	2	1
11 斑头舌鳎 C. puncticeps	Cynoglossoides	1	1	1
12 少鳞舌鳎 C. oligolepis	Cynoglossoides	2	2	1
13 宽体舌鳎 C. robustus	Cynoglossoides	2	1	1
14 日本须鳎 Paraplagusis japonica	Rhinoplagusia	1	1	1
外群 Outgroup
15 塞内加尔鳎 Sole senegalensis		3	3	2
16 人字钩嘴鳎 Heteromycteris matsubarai		2	1	2
17 圆斑星鲽 Verasper variegatus		1	1	1