Biodiv Sci ›› 2000, Vol. 08 ›› Issue (3): 337-342.  DOI: 10.17520/biods.2000048

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Molecular techniques and their application to the study of microbial diversity

YANG Yong-Hua, YAO Jian   

  1. Department of Biological Sciences and Technology , Nanjing University , Nanjing  210093
  • Received:1999-08-17 Revised:2000-05-16 Online:2000-08-20 Published:2000-08-20
  • Contact: YANG Yong-Hua

Abstract: Microbial diversity is an important part of biodiversity. Microbial diversity research and its conservation and utilization are quite different from those of macroorganisms including animals and plants. In particular , it is necessary to develop new techniques suitable for microbial diversity research. Recently , as the development of modern molecular biology , several molecular techniques have been used to study microbial diversity , including 1) total DNA extraction of microbe and its genetic diversity assay , 2) 16S rRNA gene sequence analysis , 3) nucleic acid hybridization , and 4) DNA kinetics. In order to promote extensive and intensive research on microbial diversity , it’s necessary to integrate these methods to enhance a combination of traditional and modern techniques , and to develop novel method.