Effects of plant species switching on dynamics of amylase and proteinase activity of Bemisia tabaci biotype B and Trialeurodes vaporariorum

doi:10.3724/SP.J.1003.2008.08050

Abstract

Abstract:

Bemisia tabaci biotype B (Bt) and Trialeurodes vaporariorum(Tv) are two whiteflies that often coexist on greenhouse-grown vegetable crops in northern China. The host plant species ofB. tabaci biotype B have been increasing with the spread of its invaded areas. To clarify the effects of plant species on the performances of the two species, amylase and proteinase activity dynamics of both whitefly species were evaluated when their host plant species switched from tomato (preferred by both species, also used as the control) to cotton (preferred by Bt and suitable for Tv), cabbage (preferred by Bt, but unsuitable for Tv) or maize (unsuitable for both species) plants. There were no prominent effects of plant species switching on the amylase activity of the two whitefly species. The amylase activity of Bt was 1.49-1.66 folds higher than that of Tv when fed on tomato or when switched to different host species. There were no effects on the proteinase activity of Bt when switched to a different host species, but that of Tv decreased by 29.9-42.7%. The proteinase activity of Bt was 1.30 and 1.21 folds higher than that of Tv when switched to cabbage and cotton plants, respectively. There were no interspecific differences in proteinase activity when switched to maize plants. Bt amylase activity dynamics were expressed as activation then inhibition or inhibition then activation when switched to cabbage and maize plants; however, those of Tv were smooth. Amylase activity dynamics were similar for both species when switched to cotton. Although proteinase activity dynamics of Bt were expressed as activation then inhibition when switched to any of the three plant species tested, the activation period was longer when switched to maize plants. In the case of Tv, proteinase activity dynamics were similarly smooth regardless of which plant species switched to. Our results indicated that Bt was more adaptable to plant species switching than T. vaporariorum.

Key words: Bemisia tabaci biotype B, Trialeurodes vaporariorum, amylase, proteinase, dynamics, plant species switching, adaptability

Guifen Zhang, Fang Lei, Fanghao Wan, Jun Ma, Yuguo Yang. Effects of plant species switching on dynamics of amylase and proteinase activity of Bemisia tabaci biotype B and Trialeurodes vaporariorum[J]. Biodiv Sci, 2008, 16(4): 313-320.

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URL: https://www.biodiversity-science.net/EN/10.3724/SP.J.1003.2008.08050

https://www.biodiversity-science.net/EN/Y2008/V16/I4/313

Figures/Tables 4

Table 1 Effects of plant species switching on amylase activity of Bemisia tabacibiotype B and Trialeurodes vaporariorum (mean±SE, μg•mg-1•min-1)

寄主植物种类 Host plant species	B型烟粉虱 Bemisia tabaci biotype B		温室粉虱 Trialeurodes vaporariorum
寄主植物种类 Host plant species	淀粉酶活性 Amylase activity	相对比值* Ratio		淀粉酶活性 Amylase activity	相对比值* Ratio
甘蓝 Cabbage	61.89±5.59^{a A}	0.89	38.94±4.40^{a B}		0.93
棉花 Cotton	61.00±5.04^{a A}	0.88	40.98±3.07^{a B}		0.98
玉米 Maize	70.99±8.18^{a A}	1.02	42.86±3.50^{a B}		1.02
番茄(对照) Tomato (CK)	69.49±7.54^{a A}	1.00	41.89±3.86^{a B}		1.00

Table 2 Effects of plant species switching on proteinase activity of Bemisia tabacibiotype B and Trialeurodes vaporariorum (mean±SE, µg•mg-1•min-1)

寄主植物种类 Host plant species	B型烟粉虱 Bemisia tabaci biotype B		温室粉虱 Trialeurodes vaporariorum
寄主植物种类 Host plant species	蛋白酶活性 Proteinase activity	相对比值* Ratio	蛋白酶活性 Proteinase activity	相对比值* Ratio
甘蓝 Cabbage	28.29±3.53^{a A}	0.96	21.68±2.43^{abc B}	0.70
棉花 Cotton	21.52±2.75^{a A}	0.73	17.72±2.02^{a A}	0.57
玉米 Maize	20.57±4.00^{a A}	0.70	19.17±3.44^{ab A}	0.62
番茄(对照) Tomato (CK)	29.36±3.90^{a A}	1.00	30.93±3.87^{c A}	1.00

Fig. 1 Effects of plant species switching on dynamics of amylase activity of Bemisia tabacibiotype B and Trialeurodes vaporariorum. (A), Cabbage; (B), Cotton; (C), Maize. * The data are ratios of amylase activity on tomato to other host plant species at the same testing time. Data at the same testing time followed by the same letter are not significantly different at P=0.05 between B. tabacibiotype B and T. vaporariorum when feeding on the same plant species (Paired samples ttest).

Fig. 2 Effects of plant species switching on dynamics of proteinase activity of Bemisia tabacibiotype B and Trialeurodes vaporariorum. (A), Cabbage; (B), Cotton; (C), Maize. * The data are ratios of proteinase activity on tomato to other host plant species at the same testing time. Data at the same testing time followed by the same letter are not significantly different at P=0.05 between B. tabaci biotype B and T. vaporariorum when feeding on the same plant species (Paired samples ttest).

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