Biodiv Sci ›› 2021, Vol. 29 ›› Issue (8): 1073-1086.DOI: 10.17520/biods.2021001

• Original Papers: Plant Diversity • Previous Articles     Next Articles

Effects of environmental and genetic factors on phenotypic traits and species classification of Dendrobium huoshanense

Zhenbin Jiao1,2, Yibo Luo1,*()   

  1. 1 State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093
    2 University of Chinese Academy of Sciences, Beijing 100049
  • Received:2021-01-02 Accepted:2021-03-22 Online:2021-08-20 Published:2021-05-28
  • Contact: Yibo Luo

Abstract:

Aims: Dendrobium is one of the largest genera in Orchidaceae. The species of this genus are characterized by high morphological diversity. Dendrobium huoshanense is an endemic species of China with high pharmaceutical and conservation value. However, the boundary of the species is still in debate. In this study, we aim to explore the variations of phenotypic traits affected by environmental and genetic factors, and define the boundaries among D. huoshanense and its related species.

Methods: We measured 12 phenotypic traits from 2,279 specimens in 16 populations from the dendrobiums planting base in Huoshan County, Anhui Province, East China, including wild and F1, F2 generation of D. huoshanense, wild D. henanense, D. moniliforme and D. catenatum. The F1 and F2 generations of D. huoshanense were cultivated from the seeds of random hand-pollination flowers in wild population and F1 generation population from this base, respectively. We first used common-garden experiment method and intergenerational comparison to estimate the phenotypic variations of D. huoshanense affected by environmental and genetic factors. Furthermore, the phenotypic variations of D. huoshanense and its related species were estimated by statistical analysis.

Results: The results showed that F1 and F2 generations of D. huoshanense in greenhouse and woodland conditions had larger and longer pseudobulbs than that of wild specimens under the common-garden experiment method. With the intergenerational comparison, we found that the pseudobulb diameters of F1 generation were wider than that of F2 generation. Among related species, we found that the pseudobulb lengths and diameters as well as the petals of D. huoshanense were significantly different from that of D. catenatum and D. moniliforme. At the same time, D. huoshanense only showed significant differences in morphological traits of pseudobulbs with that of D. henanense.

Conclusion: Our results suggest that both environmental and genetic factors have significant effects on phenotypic changes of D. huoshanense and affect the delineation of species boundaries among D. huoshanense and its related species.

Key words: Dendrobium, Orchidaceae, phenotypic traits, environmental factors, genetic factors