河北省安国地区丹参根围AM真菌多样性

doi:10.3724/SP.J.1003.2010.09000

摘要/Abstract

摘要：

已有研究证实, AM真菌能够促进植物生长发育, 提高植物抗病性和抗逆性。为探明药用植物丹参(Salvia miltiorrhiza)根围AM真菌资源状况, 作者分别于2008年8月和2009年8月, 在河北省安国市4个样地(齐村、中药材种植基地、祈州、沙头)采集丹参根围0-30 cm土层土壤样品, 研究根围AM真菌的生态分布、多样性及其与土壤因子的相关性。共分离鉴定出AM真菌4属24种, AM真菌最大孢子密度为77.55级/土样, 最大定殖率为72.72%。其中球囊霉属(Glomus)16种, 平均分离频度为100%, 相对多度最高达76.4%, 是4个样地的共同优势属; 无梗囊霉属(Acaulospora)5种, 分离频度及相对多度仅次于球囊霉属; 盾巨孢囊霉属(Scutellospora)2种, 仅在祈州样地出现; 内养囊霉属(Entrophospora)1种, 仅在沙头样地出现; 聚丛球囊霉(G. aggregatum)是4个样地的共同优势种。4个样地平均Shannon-Wiener指数从大到小依次为齐村、祈州、沙头、中药材种植基地; 中药材种植基地AM真菌组成与其他样地差异显著, 其孢子密度、种丰度随土壤速效P、碱解N、有机质和pH值升高而降低。上述结果显示丹参根围AM真菌资源丰富, 并与丹参形成良好的共生关系, 这为进一步筛选高效优势菌种, 利用菌根技术提高丹参产量和品质提供了依据。

关键词: 分离频度, 相对多度, 物种多样性, 土壤因子, Salvia miltiorrhiza

Abstract

Arbuscular mycorrhizal (AM) fungi can improve vigor and reduce susceptibility of hosts challenged by root pathogens. Salvia miltiorrhiza, native to China and Japan, is highly valued for its roots in traditional Chinese medicine. To elucidate species composition and diversity of AM fungi associated withS.miltiorrhiza, we collected soil samples from the rhizosphere of S. miltiorrhiza in Shatou, Zhongzhijidi, Qicun, and Qizhou in Anguo City of Hebei Province in August 2008 and 2009. A total of 24 AM fungal species belonging to four genera were isolated, of which 16 belonged to Glomus, 5 belonged to Acaulospora, 2 were Scutellosporaunique to Qizhou, and 1 belonged to Entrophospora only found in Shatou. Glomuswas the dominant genus with a frequency of 100% occurrence and a relative abundance of 76.4% in the four sites. Glomus aggregatum was the dominant species. Maximum spore density was 77.55 degrees per soil sample and maximum colonization rate was 72.72%. In Zhongzhijidi spore density and species richness decreased with soil available P and N, organic matter and increasing pH. We found a significant difference in AM fungal composition between Zhongzhijidi and other sites. Shannon-Wiener indices at the four sites listed in descending order were: Qicun, Qizhou, Shatou and Zhongzhijidi. Our results provide some of the first descriptions of the abundant AM fungal species associated withS. miltiorrhiza.

Key words: frequency, relative abundance, species diversity, soil factors, Salvia miltiorrhiza

贺学礼, 王凌云, 马晶, 赵丽莉 (2010) 河北省安国地区丹参根围AM真菌多样性. 生物多样性, 18, 175-181. DOI: 10.3724/SP.J.1003.2010.09000.

Xueli He, Lingyun Wang, Jing Ma, Lili Zhao (2010) AM fungal diversity in the rhizosphere of Salvia miltiorrhiza in Anguo City of Hebei Province. Biodiversity Science, 18, 175-181. DOI: 10.3724/SP.J.1003.2010.09000.

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链接本文: https://www.biodiversity-science.net/CN/10.3724/SP.J.1003.2010.09000

https://www.biodiversity-science.net/CN/Y2010/V18/I2/175

图/表 6

参考文献 32

[1]	Abbot LK, Robson AD (1985) Formation of external hyphae and soil by four species of vesicular-arbuscular mycorrhizal fungi. New Phytologist, 99,245-255.
[2]	Camargo-Ricalde SL, Dhillion SS (2003) Endemic Mimosa species can serve as mycorrhizal “resource islands” within semiarid communities of the Tehuacán-Cuicatlán Valley, Mexico. Mycorrhiza, 13,129-136. DOI URL PMID
[3]	Cheng LT (程俐陶), Liu ZY (刘作易), Guo QS (郭巧生), Zhu GS (朱国胜) (2009) Advances in studies on arbuscular mycorrhizas in medicinal plant. Chinese Traditional and Herbal Drug (中草药), 40(1),156-160. (in Chinese with English abstract)
[4]	Fontenla S, Godoy R, Rosso P (1998) Root associations in Austrocedrus forests and seasonal dynamics of arbuscular mycorrhizas . Mycorrhiza, 8,29-33.
[5]	Gai JP (盖京苹), Feng G (冯固), Li XL (李晓林) (2004) Diversity of arbuscular mycorrhizal fungi in field soils from North China. Biodiversity Science (生物多样性), 12,435-440. (in Chinese with English abstract)
[6]	He XL (贺学礼), Li J (李君), He C (贺超) (2009) Effects of AM fungi on the chemical components of Salvia miltiorrhiza Bge. under different N-applied levels . Chinese Agricultural Science Bulletin (中国农学通报), 25,182-185. (in Chinese with English abstract)
[7]	He XL (贺学礼), Li B (李斌) (1999) Selection research of VA mycorrhizal fungus and plant. Acta Botanica Boreali-Occidentalia Sinica (西北植物学报), 19,471-475. (in Chinese with English abstract)
[8]	Ianson DC, Allen MF (1986) The effects of soil texture on extraction of vesicular arbuscular mycorrhizal spores from arid soils. Mycologia, 78,164-168.
[9]	Lambert DH, Cloe H, Baker DE (1980) Variation in the response of Alfalfa clones and cultivars of mycorrhiza and phosphorus. Crop Science, 20,615-618.
[10]	Liu RJ (刘润进), Liu PQ (刘鹏起), Xu K (徐坤) (1999) Ecological distribution of arbuscular mycorrhizal fungi in saline alkaline soils of China. Chinese Journal of Applied Ecology (应用生态学报), 10,721-724. (in Chinese with English abstract)
[11]	Liu RJ (刘润进), Chen YL (陈应龙) (2007) Mycorrhizology (菌根学). Science Press, Beijing. (in Chinese)
[12]	Lu RK (鲁如坤) (1999) Agricultural Chemical Analysis on Soil (土壤农业化学分析方法). China Agricultural Science and Technology Press, Beijing. (in Chinese)
[13]	Lu YQ (卢彦琦), He XL (贺学礼) (2008) Effects of AM fungi on biomass and nitrogen content of Atractylodes macrocephala under different nitrogen levels . Journal of Henan Agricultural Sciences (河南农业科学), 4,94-96. (in Chinese with English abstract)
[14]	Ma J (马晶), He XL (贺学礼), Jiang ZM (姜在民), Wang LY (王凌云) (2009) Influence of soil factors on arbuscular mycorrhizal fungal infection of Salvia miltiorrhiza . Acta Agriculturae Boreali-Occidentalis Sinica (西北农业学报), 18 (5),194-198. (in Chinese with English abstract)
[15]	Muthukumar T, Udaiyan K (1997) Influence of native endomycorrhiza, soil flooding and nurse plant on mycorrhizal status and growth of purple nutsedge ( Cyperus rotundus L.) . Agriculture, Ecosystems and Environment, 6,51-58.
[16]	Oehl F, Sieverding E, Mader P, Dubois D, Ineichen K, Boller T, Wiemken A (2004) Impact of long-term conventional and organic farming on the diversity of arbuscular mycorrhizal fungi. Oecologia, 138,574-583. URL PMID
[17]	Pande M, Tarafdar JC (2004) Arbuscular mycorrhizal fungal diversity in neem-based agroforestry systems in Rajasthan. Soil Ecology, 26,233-241.
[18]	Phillips JM, Hayman DS (1970) Improved procedures for clearing roots and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of the British Mycological Society, 55,158-161.
[19]	Rabatin SC, Stinner BR (1991) The significance of vesicular arbuscular mycorrhiza fungi-macroinvertebrate interactions in agroecosystems. Agriculture Ecosystems and Environment, 27,195-204.
[20]	Schenck NC, Péréz Y (1988) Manual for the Identification of VA Mycorrhizal Fungi, 2nd edn. INVAM, University of Florida, Gainesville, USA.
[21]	Schwob L, Ducher M, Coudret A (1999) Effect of climatic factors on native arbuscular mycorrhizae and Meloidogyne exigua in a Brazilian rubber tree ( Hevea brasiliensis) plantation . Plant Pathology, 48,19-25.
[22]	Shi L (石蕾), He XL (贺学礼) (2007) Effects of AM fungi on growth and the physiological characters of Astragalus mongholicus under different P-applied level . Acta Agriculturae Boreali-Occidentalis Sinica (西北农业学报), 16(1),46-50. (in Chinese with English abstract)
[23]	Smith SE, Read DJ (1997) Mycorrhizal Symbiosis, 2nd edn. Academic Press, London.
[24]	Tadych M, Blaszkowski J (2000) Arbuscular fungi and mycorrhizae (Glomales) of the Slowinski National Park, Poland. Mycotaxon, 74,463-482.
[25]	Vander Heijden MGA, Klironomos JN, Ursic M, Moutoglis P, Streitwolf-Engel R, Boller T, Wiemken A, Sanders IR (1998) Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity. Nature, 396,69-72.
[26]	Wang FY (王发园), Liu RJ (刘润进) (2001) A preliminary survey of arbuscular mycorrhizal fungi in saline-alkaline soil of the Yellow River Delta. Biodiversity Science (生物多样性), 9,389-392. (in Chinese with English abstract)
[27]	Wang MY (王淼焱), Li M (李敏), Liu ST (刘树堂), Sui FG (隋方功), Liu RJ (刘润进) (2006a) Arbuscular mycorrhizal fungi in long-term fixed fertilization field. Journal of Fungal Research (菌物研究), 4 (4),5-9. (in Chinese with English abstract)
[28]	Wang MY (王淼焱), Cong L (丛蕾), Li M (李敏), Liu RJ (刘润进) (2006b) Three new records of arbuscular mycorrhizal fungi in China. Mycosystema (菌物学报), 25,244-246. (in Chinese)
[29]	Zhang MQ (张美庆), Wang YS (王幼珊), Zhang C (张弛), Huang L (黄磊) (1994) The ecological distribution characteristics of some genera and species of VAM fungi in northern China. Acta Mycologica Sinica (真菌学报), 13,166-172. (in Chinese with English abstract)
[30]	Zhang MQ (张美庆), Wang YS (王幼珊), Xing LJ (邢礼军) (1998) The ecological distribution of AM fungi community in south and east coast of China. Mycosystema (菌物系统), 17,274-277. (in Chinese with English abstract)
[31]	Zhang MQ (张美庆), Wang YS (王幼珊), Xing LJ (邢礼军) (1999) The relationship between the distribution of AM fungi and environmental factors. Mycosystema (菌物系统), 18(1),25-29. (in Chinese with English abstract)
[32]	Zhao DD (赵丹丹), Liang CC (梁昌聪), Zhao ZW (赵之伟) (2006) Arbuscular mycorrhizas in the tributary dry-hot valleys (Puduhe and Xiaojiang) of Jinsha River. Acta Botanica Yunnanica (云南植物研究), 28,250-256. (in Chinese with English abstract)

	齐村 Qicun			祈州 Qizhou						沙头 Shatou
	F (%)	RA (%)	I (%)	F (%)	RA (%)	I (%)	F (%)	RA (%)	I (%)	F (%)	RA (%)	I (%)
球囊霉属 Glomus	100^a	79.4^a	89.7^a	100^a	88.2^a	94.1^a	100^a	64.4^a	82.2^a	100^a	73.5^a	86.8^a
无梗囊霉属 Acaulospora	100^a	20.6^b	60.3^b	100^a	12.5^b	56.3^b	100^a	35.6^b	67.8^b	100^a	26.3^b	63.2^b
盾巨孢囊霉属 Scutellospora	0.0^b	0.0^c	0.0^c	50.0^b	0.3^c	25.2^c	0.0^b	0.0^c	0.0^c	0.0^c	0.0^c	0.0^d
内养囊霉属 Entrophospora	0.0^b	0.0^c	0.0^c	0.0^c	0.0^c	0.0^d	0.0^b	0.0^c	0.0^c	27.8^b	0.2^c	14.0^c

	齐村 Qicun			祈州 Qizhou						沙头 Shatou
	F (%)	RA (%)	I (%)	F (%)	RA (%)	I (%)	F (%)	RA (%)	I (%)	F (%)	RA (%)	I (%)
球囊霉属 Glomus	100^a	79.4^a	89.7^a	100^a	88.2^a	94.1^a	100^a	64.4^a	82.2^a	100^a	73.5^a	86.8^a
无梗囊霉属 Acaulospora	100^a	20.6^b	60.3^b	100^a	12.5^b	56.3^b	100^a	35.6^b	67.8^b	100^a	26.3^b	63.2^b
盾巨孢囊霉属 Scutellospora	0.0^b	0.0^c	0.0^c	50.0^b	0.3^c	25.2^c	0.0^b	0.0^c	0.0^c	0.0^c	0.0^c	0.0^d
内养囊霉属 Entrophospora	0.0^b	0.0^c	0.0^c	0.0^c	0.0^c	0.0^d	0.0^b	0.0^c	0.0^c	27.8^b	0.2^c	14.0^c

	齐村 Qicun				祈州 Qizhou				种植基地 Zhongzhijidi				沙头 Shatou
	F (%)	RA (%)	I (%)	Dom	F (%)	RA (%)	I (%)	Dom	F (%)	RA (%)	I (%)	Dom	F (%)	RA (%)	I (%)	Dom
双网无梗囊霉 A.bireticulata	100.0	15.7	57.9	B	100.0	15.4	57.70	B	100.0	28.6	64.3	A	100.0	18.0	59.0	B
孔窝无梗囊霉 A. foveata	100.0	1.5	50.8	B	97.2	0.9	49.1	B	100.0	4.1	52.1	B	83.7	2.8	43.3	B
光壁无梗囊霉 A. laevis	41.7	0.6	21.2	C	55.6	0.4	28.0	C	0.0	0.0	0.0	-	47.2	0.6	23.9	C
瑞氏无梗囊霉 A. rehmii	86.1	4.2	45.2	B	0.0	0.0	0.0	-	0.0	0.0	0.0	-	100.0	2.6	51.3	B
细凹无梗囊霉 A. scrobiculata	50.0	1.5	25.8	C	100.0	0.9	50.5	B	16.7	0.2	8.5	D	0.0	0.0	0.0	-
聚丛球囊霉 G. aggregatum	100.0	48.3	74.2	A	100.0	42.9	71.5	A	100.0	38.0	69.0	A	100.0	34.4	67.2	A
明球囊霉 G. clarum	100.0	5.0	52.5	B	100.0	3.1	51.6	B	100.0	4.9	52.5	B	100.0	5.6	52.8	B
缩球囊霉 G. constrictum	100.0	1.8	50.9	B	75.0	1.3	38.2	C	83.3	2.9	43.1	C	91.7	2.0	46.9	B
沙荒球囊霉 G. deserticola	25.0	0.2	12.6	D	83.3	1.1	42.2	B	0.0	0.0	0.0	-	83.7	1.7	43.0	B
长孢球囊霉 G. dolichosporum	58.3	0.7	29.5	C	33.4	0.3	16.9	D	33.3	0.8	17.1	D	0.0	0.0	0.0	-
集球囊霉 G. fasciculatum	36.1	1.0	18.1	D	88.9	0.6	44.8	B	41.9	1.4	21.7	C	41.9	1.7	21.8	C
地球囊霉 G. geosporum	0.0	0.0	0.0	-	97.2	1.1	49.2	B	0.0	0.0	0.0	-	33.4	0.5	17.0	D
斑点球囊霉 G. maculosum	0.0	0.0	0.0	-	0.0	0.0	0.0	-	33.3	0.3	16.8	D	33.3	0.2	16.8	D
微丛球囊霉 G. microaggregatum	100.0	1.3	50.6	B	100.0	1.7	50.9	B	0.0	0.0	0.0	-	50.0	0.2	25.1	C
摩西球囊霉 G. mosseae	100.0	8.3	54.2	B	100.0	8.8	54.4	B	100.0	14.1	57.1	B	100.0	17.0	58.5	B
多梗球囊霉 G. multicaule	0.0	0.0	0.0	-	33.3	0.8	17.1	D	0.0	0.0	0.0	-	0.0	0.0	0.0	-
凹坑球囊霉 G. multiforum	100.0	1.4	50.7	B	33.3	8.2	20.8	C	0.0	0.0	0.0	-	66.7	0.7	33.7	C
网状球囊霉 G. reticulatum	100.0	14.5	57.3	B	100.0	26.2	63.1	A	100.0	12.8	56.4	B	100.0	12.9	56.5	B
扭形球囊霉 G. tortuosum	100.0	1.3	50.7	B	0.0	0.0	0.0	-	50.0	0.6	25.3	C	0.0	0.0	0.0	-
地表球囊霉 G. versiforme	0.0	0.0	0.0	-	0.0	0.0	0.0	-	16.7	0.1	8.4	D	0.0	0.0	0.0	-
粘质球囊霉 G. viscosum	50.0	0.6	25.3	C	0.0	0.0	0.0	-	0.0	0.0	0.0	-	0.0	0.0	0.0	-
丽孢盾巨孢囊S.calospora	0.0	0.0	0.0	-	66.7	0.3	33.5	C	0.0	0.0	0.0	-	0.0	0.0	0.0	-
红色盾巨孢囊霉S. erythropa	0.0	0.0	0.0	-	33.3	0.2	16.7	D	0.0	0.0	0.0	-	0.0	0.0	0.0	-
稀有内养囊霉 E. infrequens	0.0	0.0	0.0	-	0.0	0.0	0.00	-	0.0	0.0	0.0	-	41.9	0.7	21.3	C

	齐村 Qicun				祈州 Qizhou				种植基地 Zhongzhijidi				沙头 Shatou
	F (%)	RA (%)	I (%)	Dom	F (%)	RA (%)	I (%)	Dom	F (%)	RA (%)	I (%)	Dom	F (%)	RA (%)	I (%)	Dom
双网无梗囊霉 A.bireticulata	100.0	15.7	57.9	B	100.0	15.4	57.70	B	100.0	28.6	64.3	A	100.0	18.0	59.0	B
孔窝无梗囊霉 A. foveata	100.0	1.5	50.8	B	97.2	0.9	49.1	B	100.0	4.1	52.1	B	83.7	2.8	43.3	B
光壁无梗囊霉 A. laevis	41.7	0.6	21.2	C	55.6	0.4	28.0	C	0.0	0.0	0.0	-	47.2	0.6	23.9	C
瑞氏无梗囊霉 A. rehmii	86.1	4.2	45.2	B	0.0	0.0	0.0	-	0.0	0.0	0.0	-	100.0	2.6	51.3	B
细凹无梗囊霉 A. scrobiculata	50.0	1.5	25.8	C	100.0	0.9	50.5	B	16.7	0.2	8.5	D	0.0	0.0	0.0	-
聚丛球囊霉 G. aggregatum	100.0	48.3	74.2	A	100.0	42.9	71.5	A	100.0	38.0	69.0	A	100.0	34.4	67.2	A
明球囊霉 G. clarum	100.0	5.0	52.5	B	100.0	3.1	51.6	B	100.0	4.9	52.5	B	100.0	5.6	52.8	B
缩球囊霉 G. constrictum	100.0	1.8	50.9	B	75.0	1.3	38.2	C	83.3	2.9	43.1	C	91.7	2.0	46.9	B
沙荒球囊霉 G. deserticola	25.0	0.2	12.6	D	83.3	1.1	42.2	B	0.0	0.0	0.0	-	83.7	1.7	43.0	B
长孢球囊霉 G. dolichosporum	58.3	0.7	29.5	C	33.4	0.3	16.9	D	33.3	0.8	17.1	D	0.0	0.0	0.0	-
集球囊霉 G. fasciculatum	36.1	1.0	18.1	D	88.9	0.6	44.8	B	41.9	1.4	21.7	C	41.9	1.7	21.8	C
地球囊霉 G. geosporum	0.0	0.0	0.0	-	97.2	1.1	49.2	B	0.0	0.0	0.0	-	33.4	0.5	17.0	D
斑点球囊霉 G. maculosum	0.0	0.0	0.0	-	0.0	0.0	0.0	-	33.3	0.3	16.8	D	33.3	0.2	16.8	D
微丛球囊霉 G. microaggregatum	100.0	1.3	50.6	B	100.0	1.7	50.9	B	0.0	0.0	0.0	-	50.0	0.2	25.1	C
摩西球囊霉 G. mosseae	100.0	8.3	54.2	B	100.0	8.8	54.4	B	100.0	14.1	57.1	B	100.0	17.0	58.5	B
多梗球囊霉 G. multicaule	0.0	0.0	0.0	-	33.3	0.8	17.1	D	0.0	0.0	0.0	-	0.0	0.0	0.0	-
凹坑球囊霉 G. multiforum	100.0	1.4	50.7	B	33.3	8.2	20.8	C	0.0	0.0	0.0	-	66.7	0.7	33.7	C
网状球囊霉 G. reticulatum	100.0	14.5	57.3	B	100.0	26.2	63.1	A	100.0	12.8	56.4	B	100.0	12.9	56.5	B
扭形球囊霉 G. tortuosum	100.0	1.3	50.7	B	0.0	0.0	0.0	-	50.0	0.6	25.3	C	0.0	0.0	0.0	-
地表球囊霉 G. versiforme	0.0	0.0	0.0	-	0.0	0.0	0.0	-	16.7	0.1	8.4	D	0.0	0.0	0.0	-
粘质球囊霉 G. viscosum	50.0	0.6	25.3	C	0.0	0.0	0.0	-	0.0	0.0	0.0	-	0.0	0.0	0.0	-
丽孢盾巨孢囊S.calospora	0.0	0.0	0.0	-	66.7	0.3	33.5	C	0.0	0.0	0.0	-	0.0	0.0	0.0	-
红色盾巨孢囊霉S. erythropa	0.0	0.0	0.0	-	33.3	0.2	16.7	D	0.0	0.0	0.0	-	0.0	0.0	0.0	-
稀有内养囊霉 E. infrequens	0.0	0.0	0.0	-	0.0	0.0	0.00	-	0.0	0.0	0.0	-	41.9	0.7	21.3	C

	祈州 Qizhou	种植基地 Zhongzhijidi	沙头 Shatou
齐村 Qicun	0.80^a	0.62^c	0.76^b
祈州 Qizhou		0.61^c	0.75^b
种植基地 Zhongzhijidi			0.60^c