生物多样性 ›› 2025, Vol. 33 ›› Issue (2): 24541. DOI: 10.17520/biods.2024541 cstr: 32101.14.biods.2024541
• • 上一篇
收稿日期:
2024-12-06
接受日期:
2025-02-21
出版日期:
2025-02-20
发布日期:
2025-03-25
通讯作者:
*E-mail: Received:
2024-12-06
Accepted:
2025-02-21
Online:
2025-02-20
Published:
2025-03-25
Contact:
*E-mail: 摘要:
我从事分类学研究60年, 从《中国植物志》到世界专著性研究, 受到国内外的肯定。在这一过程中体会到, 完善而合理的研究方法至关重要。分类学是一门综合性很强的学科, 涉及到生物学几乎所有学科, 其中形态学、孢粉学、细胞学、遗传学、分子生物学和统计学是必须掌握的学科。针对中国的植物分类学研究历史和现状, 我以世界芍药属(科) (Paeonia, Paeoniaceae)和桔梗科党参属(Codonopsis, Campanulaceae)以及近缘类群的研究为例, 应用了上述各个学科, 重点强调野外考察和统计分析是不可或缺的研究方法。只有通过野外考察才能获取必要的实验材料, 才能分清居群多态性和种间差异; 唯有统计分析才能区分是种内的变异还是遗传隔离。分子系统学的兴起已使分类学进入一个崭新的时代, 分类学工作者必须认识它, 学习它。我的团队在上述两项研究中应用了它, 获得超预期的成果。我的感想是: 高水平的研究必须有完善的科学方法。
洪德元 (2025) 分类学中的方法论小叙. 生物多样性, 33, 24541. DOI: 10.17520/biods.2024541.
Hong Deyuan (2025) A brief discussion on methodology in taxonomy. Biodiversity Science, 33, 24541. DOI: 10.17520/biods.2024541.
图1 桔梗科沙参属泡沙参(Adenophora potaninii Korsh.)居群内叶形的多态性。A. 原亚种subsp. potaninii; B. 多歧沙参subsp. wawreana (Zahlbr.) S. Ge & D.Y. Hong。A和B分别表示一个居群的取样; a和b与c和d分别表示一个居群两个个体的叶形及其后代的叶形(引自葛颂和洪德元, 1995)。
Fig. 1 Population polymorphism of leaf morphology in Adenophora potaninii Korsh. (Campanulaceae). A. subsp. potaninii; B. subsp. wawreana (Zahlbr.) S. Ge & D.Y. Hong. A & B, respectively showing sampling of a population. a, b, c & d respectively showing leaf morphology of two individuals from one population and leaf morphology of their offspring (Ge & Hong, 1995).
图2 滇牡丹(Paeonia delavayi Franch.)云南中甸(香格里拉)两个居群内花色的多态性。A. 哈那村居群; B. 翁水居群(Hong et al, 1998)。
Fig. 2 Population polymorphism of petals in two populations of Paeonia delavayi Franch. in Shangri-la county, Yunnan. A. Hana Village population; B. Wengshui population (Hong et al, 1998).
图3 由4个叶绿体DNA片段构建的分子系统树。树上附有外部形态(花盘或腺体有否)、花粉类型(长沟或短沟)和染色体(2n = 14或16)。图示来自4个学科(外部形态、孢粉学、细胞学、分子生物学)信息的融合(Wang et al, 2014)。
Fig. 3 Molecular phylogenetic tree constructed from four chloroplast DNA fragments. The tree is annotated with external morphology (presence or absence of floral disc or glands), pollen morphology (long colpus or short colpus), and chromosome number (2n = 14 or 16). The illustration demonstrates the integration of information from four disciplines (external morphology, palynology, cytology, and molecular biology (Wang et al, 2014).
图4 对滇牡丹(Paeonia delavayi Franch.) 12个居群的叶裂片数(A)和叶裂片宽度(B)作的标准差分析(引自Hong et al, 1998)
Fig. 4 Standard deviation of 12 populations for Paeonia delavayi Franch.: A. Number of leafy segments; B. Width of leaf segments (Hong et al, 1998; with permission)
[1] | APG IV (2016) An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG IV. Botanical Journal of the Linnean Society, 181, 1-20. |
[2] | CBOL Plant Working Groups (2009) A DNA barcode for land plants. Proceedings of the National Academy of Sciences, USA, 106, 12794-12797. |
[3] | Chase MW, Soltis DE, Olmstead RG, Morgan D, Les DH, Mishler BD, Duvall MR, Price RA, Hills HG, Qiu Y-L, Kron KA, Rettig JH, Conti E, Palmer JD, Manhart JR, Sytsma KJ, Michaels HJ, Kress WJ, Karol KG, Clark WD, Hedren M, Gaut BS, Jansen RK, Kim KJ, Wimpee CF, Smith JF, Furnier GR, Strauss SH, Xiang QY, Plunkett GM, Soltis PS, Swensen SM, Williams SE, Gadek PA, Quinn CJ, Eguiarte LE, Golenberg E, Learn GH, Graham SW, Barrett SCH, Dayanandan S, Albert VA (1993) Phylogenetics of seed plants: An analysis of nucleotide sequences from the plastid gene rbcL. Annals of the Missouri Botanical Garden, 80, 528-580. |
[4] | Fang WP (1958) Notes on Chinese paeonies. Acta Phytotaxonomica Sinica, 7, 297-323, Pl. LXI-LXIII. (in Chinese and in English) |
[ 方文培 (1958) 中国芍药属的研究. 植物分类学报, 7, 297-323, Pl. LXI-LXIII.] | |
[5] | Ge S, Hong DY (1995) Biosystematic studies on Adenophora potaninii Korsh. complex (Campanulaceae). III. Genetic variation and taxonomic value of morphological characters. Acta Phytotaxonomica Sinica, 33, 433-443. (in Chinese with English abstract) |
[ 葛颂, 洪德元 (1995) 泡沙参复合体(桔梗科)的物种生物学研究. Ⅲ. 性状的遗传变异及其分类价值. 植物分类学报, 33, 433-443.] | |
[6] | Haw SG (2001) Paeonia delavayi, a variable species. The New Plantsman, 8, 251-253. |
[7] | Hong DY (1984) Echinocodon Hong, a new genus of Campanulaceae and its systematic position. Acta Phytotaxonomica Sinica, 22, 181-184. (in Chinese with English abstract) |
[ 洪德元 (1984) 刺萼参属——桔梗科一新属及其系统位置. 植物分类学报, 22, 181-184.] | |
[8] | Hong DY (1997) Paeonia (Paeoniaceae) in Xizang (Tibet). Novon, 7, 156-161. |
[9] | Hong DY (2010) Peonies of the World:Taxonomy and Phytogeography. Royal Botanical Garden, Kew Publishing, London & Missouri Botanical Garden Press, St. Louis. |
[10] | Hong DY (2011) Paeonia rotundiloba (D. Y. Hong) D. Y. Hong: A new status in tree peonies (Paeoniaceae). Journal of Systematics and Evolution, 49, 464-467. |
[11] | Hong DY (2015) A Monograph of Codonopsis and Allied Genera (Campanulaceae). Elsevier, Amsterdam. |
[12] | Hong DY (2016) Biodiversity pursuits need a scientific and operative species concept. Biodiversity Science, 24, 979-999. (in Chinese with English abstract) |
[ 洪德元 (2016) 生物多样性事业需要科学、可操作的物种概念. 生物多样性, 24, 979-999.]
DOI |
|
[13] | Hong DY (2021) Peonies of the World III:Phylogeny and Evolution. Royal Botanical Gardens, Kew Publishing, London & Missouri Botanical Garden Press, St. Louis. |
[14] | Hong DY, Pan KY (2012) Pollen morphology of the Platycodonoid group (Campanulaceae s. str.) and its systematic implications. Journal of Integrative Plant Biology, 54, 773-789. |
[15] | Hong DY, Pan KY (1998) The restoration of the genus Cyclocodon (Campanulaceae) and its evidence from pollen and seed-coat. Journal of Systematics and Evolution, 36, 106-110. |
[16] | Hong DY, Pan KY, Yu H (1998) Taxonomy of the Paeonia delavayi complex (Paeoniaceae). Annals of the Missouri Botanical Garden, 85, 554-564. |
[17] | Jansen RK, Palmer JD (1987) A chloroplast DNA inversion marks an ancient evolutionary split in the sunflower family (Asteraceae). Proceedings of the National Academy of Sciences, USA, 84, 5818-5822. |
[18] | Lammers TG (1998) Campanulaceae.In: Flora Taiwan (Vol. 4) (ed. Huang TC), pp. 775-802 Editorial Committee of the Flora of Taiwan, Department of Botany, Taiwan University, Taipei. |
[19] | Lammers TG (2007) Campanulaceae. In: The Families and Genera of Vascular Plants Flowering Plants (Vol. 8): Eudicots, Asterales (ed. Kubitzki K), pp. 26-56. Springer, Berlin. |
[20] | Li DZ (2018) A Dictionary of the Families and Genera of Chinese Vascular Plants. Science Press, Beijing. (in Chinese) |
[ 李德铢 (2018) 中国维管植物科属词典. 科学出版社, 北京.] | |
[21] | Mabberley D (2008/2017) Mabberley’s Plant-book: A Portable Dictionary of Plants, Their Classification and Uses. Cambridge University Press, Cambridge, UK. |
[22] | Pan KY (1979) Paeonia. In: Flora Reipublicae Polularies Sinicae, Tomus 27 (ed. Delectis Florae Reipublicae Popularis Sinicae Agendae Academicae Sinicae Edita), pp. 37-59. Science Press. Beijing. (in Chinese) |
[ 潘开玉 (1979) 芍药属. 见: 中国植物志, 第二十七卷 (中国科学院中国植物志编辑委员会), 37-59页. 科学出版社, 北京.] | |
[23] |
Saunders AP, Stebbins GL (1938) Cytogenetic studies in Paeonia I. The compatibility of the species and the appearance of the hybrids. Genetics, 23, 65-82.
DOI PMID |
[24] | Stern FC (1946) A Study of the Genus Paeonia. The Royal Horticultural Society, London. |
[25] |
Wang Q, Ma XT, Hong DY (2014) Phylogenetic analyses reveal three new genera of the Campanulaceae. Journal of Systematics and Evolution, 52, 541-550.
DOI |
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