生物多样性 ›› 2015, Vol. 23 ›› Issue (1): 50-60.DOI: 10.17520/biods.2014089

• 研究报告: 动物多样性 • 上一篇    下一篇

基于线粒体COI和16S片段序列的北部湾北部水螅水母DNA条形码分析

张珰妮3, 郑连明1,2,3,*(), 何劲儒3, 张文静1,3, 林元烧1,3, 李阳3   

  1. 1 厦门大学海洋生物多样性与全球变化研究中心, 福建厦门 361102
    2 福建省海陆界面生态环境重点实验室, 福建厦门 361102
    3 厦门大学海洋与地球学院, 福建厦门 361102
  • 收稿日期:2014-05-09 接受日期:2014-11-19 出版日期:2015-01-20 发布日期:2015-05-04
  • 通讯作者: 郑连明
  • 作者简介:E-mail: zhlm@xmu.edu.cn
  • 基金资助:
    国家自然科学基金青年科学基金(41006078);海洋公益性行业专项(201005012-3);海洋公益性行业专项(201005015-5);厦门大学中央高校基本科研业务费专项资金(2010121037);厦门大学海洋科学基地科研训练及科研能力提高项目(J1210050)

DNA barcoding of hydromedusae in northern Beibu Gulf for species identification

Dangni Zhang3, Lianming Zheng1,2,3,*(), Jinru He3, Wenjing Zhang1,3, Yuanshao Lin1,3, Yang Li3   

  1. 1 Marine Biodiversity and Global Change Research Center, Xiamen University, Xiamen, Fujian 361102
    2 Fujian Provincial Key Laboratory for Coastal Ecology and Environmental Studies (CEES), Xiamen University, Xiamen, Fujian 361102
    3 College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian 361102;
  • Received:2014-05-09 Accepted:2014-11-19 Online:2015-01-20 Published:2015-05-04
  • Contact: Lianming Zheng

摘要:

水螅水母类是浮游动物群落的重要组成部分, 在近岸海洋生态系统物质循环和能量流动中扮演着重要角色。水螅水母类形态结构简单, 但其物种的准确鉴定一直是分类工作中的难点。DNA条形码极大地促进了水螅水母物种的快速、准确鉴定。本研究扩增了北部湾北部28种水螅水母的线粒体COI和16S序列, 分别为92条和116条; 比较了2个基因片段的种内、种间K2P (Kimura 2-parameter)遗传距离; 构建了基于这2个基因片段的系统发育邻接树(neighbor-joining phylogenetic tree); 并结合矢量分析构建了Klee-diagram图。结果显示: COI序列的种内遗传距离为0.008±0.005(0-0.033), 种间遗传距离为0.298±0.128 (0.092-0.597); 16S序列的种内遗传距离为0.006± 0.010(0-0.047), 种间遗传距离为0.394±0.195(0.068-0.898)。2个基因序列在所调查种类中, 种内遗传差异均小于种间遗传差异, 存在明显的条形码间隔(barcoding gap)。基于2个基因片段的NJ树均显示, 单种所有个体都位于同一独立分枝。研究结果表明, 以COI和16S作为DNA条形码均能对北部湾北部常见水螅水母类进行物种鉴定。

关键词: DNA条形码, 分子标记, 矢量分析, 物种鉴定, 水螅水母

Abstract

Being a major component of coastal zooplankton assemblages, hydromedusae play a key role in material recycling and energy flow of marine ecosystems. Species identification is challenging for this group due to their phonetic simplicity. DNA barcoding provides an efficient method for species identification. In the present study, 92 COI and 116 16S sequences from 28 hydromedusae species were amplified. A neighbor- joining phylogenetic tree was constructed based on Kimura 2-parameter genetic distance and further studied using Klee-diagram vector analysis. Intra-specific K2P genetic distance averaged 0.008±0.005 (0-0.033) for COI, and 0.006±0.010 (0-0.047) for 16S; inter-specific K2P genetic distance averaged 0.298±0.128 (0.092- 0.597), and 0.394±0.195 (0.068-0.898) for COI and 16S, respectively. An obvious “barcoding gap” was detected for all species in both markers and all individuals of a species clustered together in both the COI and 16S trees. Further confirmatory evidence was also provided through indicator vector analysis. Hence, both COI and 16S appear to be accurate and efficient markers for hydromedusae identification in northern Beibu Gulf.

Key words: DNA barcoding, molecular marker, vector analysis, species identification, hydromedusae