Biodiv Sci ›› 2019, Vol. 27 ›› Issue (9): 993-1001.DOI: 10.17520/biods.2019135

• Original Papers • Previous Articles     Next Articles

Species diversity and seasonal fluctuation of entomogenous fungi of Ascomycota in Taibaoshan Forest Park in western Yunnan

Zihong Chen1,Yuanbing Wang2,Yongdong Dai2,Kai Chen1,Ling Xu1,*(),Qingcheng He3,*()   

  1. 1 Institute of Biological Resources of Gaoligong Mountains, Baoshan University, Baoshan, Yunnan 678000
    2 Yunnan Herbal Laboratory, Institute of Herbal Biotic Resources, Yunnan University, Kunming 650091
    3 Editorial Office of Journal of Yunnan Agricultural University, Kunming 650201
  • Received:2019-04-18 Accepted:2019-06-21 Online:2019-09-20 Published:2019-09-25
  • Contact: Ling Xu,Qingcheng He

Abstract:

Yunnan Province and the Tibetan Plateau are home to a large diversity of entomogenous fungi. We selected Taibaoshan Forest Park of Baoshan City, in Yunnan to investigate the species diversity of entomogenous fungi of Ascomycota and their seasonal fluctuations throughout the year. We collected samples of soil and infected insects monthly in 2016, and the entomogenous fungal strains were isolated before multigene (nrSSU, nrLSU, EF-1α, RPB1, and RPB2) phylogenetic analyses were used to identify species. Finally we quanitifed the fluctuations throughout the year in alpha diversity. Entomogenous fungi of Ascomycota in Taibaoshan Forest Park were found through the entire year and we identified a total of 395 fungal strains, including 24 species from nine genera of three families. Cordyceps (199 strains of six species) and Beauveria (80 strains of five species) were the dominant genera. Akanthomyces sp., Cordyceps tenuipes, C. cicadae, C. fumosorosea and Beauveria bassiana were the dominant species. The detection rate of strains was the highest (85 strains, 21.4% of the total) in July, But the species richness (15 species) and Shannon-Wiener diversity index (2.35) were the highest in August. Among the five most dominant species, only C. cicadae was much affected by seasonal changes: occurring from May to September and being most prosperous in July (35 strains, 41.2% of the total strains in July). The other four species were detected through most of the year (11 or 12 months), indicating a constant abundance of entomogenous Ascomycota were abundant in Taibaoshan Forest Park, regardless of seasonal changes.

Key words: Taibaoshan Forest Park, entomogenous fungi, species diversity, dominant genera and species, population dynamics