Biodiversity Science ›› 2011, Vol. 19 ›› Issue (4): 476-484.doi: 10.3724/SP.J.1003.2011.09002

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Diversity and plant growth promoting activities of the cultivable rhizo-bacteria of Dongxiang wild rice (Oryza rufipogon)

Fei Luo1, 2, Ya Wang2, Qinggui Zeng1, Riming Yan1, Zhibin Zhang1, Du Zhu1, 2*   

  1. 1Key Laboratory of Protection and Utilization of Subtropical Plant Resources of Jiangxi Province, Jiangxi Normal Uni-versity, Nanchang 330022

    2Key Laboratory for Research on Active Ingredients in Natural Medicine of Jiangxi Province, Yichun University, Yichun 336000
  • Received:2011-01-10 Revised:2011-05-01 Online:2011-07-29
  • Du Zhu E-mail:zhudu@jxnu.edu.cn

In order to understand the diversity of cultivable rhizobacteria associated with Dongxiang wild rice at different growth stages and to discover new species and strains of plant growth-promoting rhizobacte-ria (PGPR), we isolated 118 bacteria strains with different colony morphology using the dilution plate count-ing method and tested their plant growth promoting activities (PGPA) in May, August and November 2009. Phylogenetic analysis based on partial 16S rRNA sequences indicated that 118 strains clustered into 11 gen-era, and the dominant bacteria genera were Bacillus, Pantoea and Sphingomonas. The rhizobacteria dis-played a high diversity with Shannon-Wiener indices of 2.037–2.741. PGPA analyses indicated that most strains displayed PGPR effects, including nitrogen-fixation, phosphorus-solubilization, IAA-production and siderophore-secretion, and that the dominant PGPR was Pantoea. Additionally, we found that the total amount and PGPA of cultivable rhizobacteria began to increase gradually at seedling stage of Dongxiang wild rice, and reached its peak at heading stage, then decreased at grain filling stage. Our results show that the diversity of bacterial communities in the rhizosphere soil of Dongxiang wild rice is abundant, and that the rich rhizobacteria may be a fascinating PGPR reservoir.

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