Biodiv Sci ›› 2019, Vol. 27 ›› Issue (4): 366-372.DOI: 10.17520/biods.2018332

• Original Papers: Plant Diversity • Previous Articles     Next Articles

Assembling and analysis of Sanicula orthacantha chloroplast genome

Chen Zhixiang1,Yao Xueying1,Stephen R. Downie2,Wang Qizhi1,*()   

  1. 1 Department of Horticulture, Huaqiao University, Xiamen, Fujian 361021, China
    2 Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana 61801
  • Received:2018-12-18 Accepted:2019-01-09 Online:2019-04-20 Published:2019-06-05
  • Contact: Wang Qizhi

Abstract:

Sanicula orthacantha is a perennial herb widely distributed in China and is a widely used for medicinal purposes. In this study, the whole chloroplast genome was sequenced and analyzed using bioinformatics methods. The results showed that it was a circular molecule 157,163 bp in length with a typical quadripartite structure including a pair of inverted repeats (IRa and IRb) of 26,247 bp that were separated by large and small single copy regions (LSC and SSC) of 87,547 bp and 17,122 bp, respectively. A total of 129 predicted genes, including 84 protein-coding genes, 37 tRNA genes and eight rRNA genes were identified. Compared with other Apiaeceae species, the S. orthacantha chloroplast genome had few differences in size, order and structure. The success of sequencing the S. orthacantha chloroplast genome provides a new method for the complete chloroplast genome assembly and characterization of Sanicula species, providing a methodological guide for the study of Sanicula plant evolution and phylogeny.

Key words: high-throughput sequencing, Sanicula L., gene assembly, phylogenetic analysis