Biodiversity Science ›› 2018, Vol. 26 ›› Issue (6): 611-619.doi: 10.17520/biods.2017268

• Original Papers • Previous Article     Next Article

Difference in pH value and nutrient and bacterial diversity in the Carya cathayensis forest soil under different management models

Mei Zhang, Mashui Lin, Xiuxiu Cao, Shumin Zhao, Daqing Jiang, Bingxuan Wang, Shiying Wang, Yandi Fan, Ming Guo, Haiping Lin*()   

  1. Local and National Joint Engineering Laboratory of Biopesticide High-Efficient Preparation, Zhejiang Agriculture and Forestry University, Lin’an, Zhejiang 311300
  • Received:2017-10-11 Accepted:2018-03-08 Online:2018-09-11
  • Lin Haiping
  • About author:

    # Co-first authors

To optimize management of the Carya cathayensis forest through the diversity and function of soil bacteria, disease index, soil pH, and nutrient and soil bacterial diversity were investigated under ecological management and excessive management during the Botryosphaeria dothidea infected period using 16S rRNA gene high-throughput sequencing technology. The correlations between each interrelationship factors were also analyzed. Results showed that the disease index of the ecologically managed forest was 3.3±3.35, which was significantly lower than that of the excessively managed forest with 81.9±1.27. Moreover, ecological management significantly improved the ability of hickory forest resistant to B. dothidea. The soil pH value of the ecologically managed forest was near neutral with a reading of 6.64±0.06, which was significantly higher than that of excessively managed forest soil with 5.80±0.04. The contents of available phosphorus, available potassium, and available nitrogen in the excessively managed forest soil were 18.10±0.58 mg/kg, 698.63±11.24 mg/kg and 227.13±3.81 mg/kg, which were significantly higher than those in the ecologically managed forest respectively, with 14.94±0.27 mg/kg, 497.13±6.19 mg/kg and 195.28±6.01 mg/kg. There were 14 and 21 major bacterial genera found in the ecologically and excessively managed forest soil samples, respectively. In ecologically managed forest soils, the dominant genera were Sphingomonas, Gaiella and Lysobacter, and the relative abundance of these was significantly higher than excessively managed forest soils. Bryobacter, Candidatus Solibacter, and Bradyrhizobium were the dominant genera in the excessively managed forest soils, and their relative abundance was significantly higher than the ecologically managed forest soils. There was no significant difference of OTUs or Ace, Chao, Shannon and Simpson indices between ecologically and excessively managed forest soils. Redundancy analysis (RDA) and Monte Carlo testing showed that pH value, available phosphorous and available nitrogen had significant effects on the bacterial flora of the dominant bacterial species in the soil (P < 0.05). Our results can provide valuable references for the sustainable management of hickory forests.

Key words: ecological management, excessive management, Carya cathayensis canker disease, bacterial diversity, pH value, soil nutrient

Table 1

The comparison of pH value, nutrient contents and bacterial diversity indices of hickory forest under different management models"

pH 6.64 ± 0.06 a 5.80 ± 0.04 b
速效磷 AP (mg/kg) 14.94 ± 0.27 b 18.10 ± 0.58 a
速效钾 AK (mg/kg) 497.13 ± 6.19 b 698.63 ± 11.24 a
速效氮 AN (mg/kg) 195.28 ± 6.01 b 227.13 ± 3.81 a
有机碳 OC (g/kg) 36.27 ± 1.58 a 37.57 ± 6.13 a
OTUs 3,786 ± 82 a 3,569 ± 128 a
Ace指数 Ace index 4,198 ± 136.80 a 4,044 ± 320.22 a
Chao指数 Chao index 4,117 ± 152.61 a 4,032 ± 304.55 a
Shannon指数 Shannon index 9.861 ± 0.116 a 9.648 ± 0.079 a
Simpson指数 Simpson index 0.9969 ± 0.0003 a 0.9965 ± 0.0003 a

Fig. 1

Species relative abundance clustering heatmap of bacteria of hickory forest soil under different management models. The corresponding values in heatmap are Z values which are obtained after standardized treatment of the relative abundance of each row of the species."

Table 2

The differences of dominant bacterial flora and relative abundance of hickory forest soil under different management models"

Bacterial flora
生态经营 Ecological management (%) 过度经营Excessive management (%)
Sphingomonas 2.29 ± 0.18 a 1.47 ± 0.27 b
Gaiella 0.61 ± 0.07 a 0.41 ± 0.06 b
Lysobacter 0.57 ± 0.10 a 0.26 ± 0.05 b
Bryobacter 0.80 ± 0.08 b 1.03 ± 0.03 a
Candidatus Solibacter 0.87 ± 0.02 b 1.40 ± 0.07 a
Bradyrhizobium 0.67 ± 0.09 b 0.95 ± 0.07 a

Fig. 2

Correlation of the dominant bacterial flora between the genus level and environmental variables of hickory forest soil under different management models. AN, Available nitrogen; AP, Available phosphorus; AK, Available potassium; OC, Organic carbon."

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