Biodiversity Science ›› 2015, Vol. 23 ›› Issue (5): 665-672.doi: 10.17520/biods.2014158

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• Orginal Article • Previous Article     Next Article

Eupatorium adenophorum invasion alters soil bacterial community and diversity

Xunzhi Zhu1, 2, Qiang Li1, Yangping Li3, Hongbo Han4, Keping Ma2, *()   

  1. 1 College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018
    2 State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093
    3 Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan 650223
    4 School of Biological and Chemical Engineering, Panzhihua University, Panzhihua, Sichuan 617000
  • Received:2015-02-03 Accepted:2015-04-18 Online:2015-10-12
  • Ma Keping E-mail:kpma@ibcas.ac.cn

The invasion of alien species can affect biological community structure and ecosystem functioning, but the relationships between invasive plants and soil microbial composition and diversity are still unclear. In order to examine the effect of the invasion of an exotic plant Eupatorium adenophorum on soil chemical properties and microbial community structure and diversity, we compared the differences in soil microbial community of three communities with different densities of E. adenophorum (native plant community, E. adenophorum and native plant mixed community, and E. adenophorum dominated community) by high-throughput sequencing. Analysis of soil chemical properties showed that soil pH, organic matter, total nitrogen and total potassium decreased with increasing invasion of E. adenophorum. However, total phosphorus was highest in the most heavily invaded soil. The high-throughput sequencing results showed that there were 7,755 soil microbial OTUs (operational taxonomic unit) in total. The invasion of E. adenophorum did not exert heavy impacts on soil microbial diversity. ACE index and Chao index showed no significant differences among the three different communities. However, soil microbial diversity of the mixed community of E. adenophorum and native plant species showed the lowest Shanonn index. The relative abundances of Acidobacteria and Verrucomicrobia in the medium invaded community were the highest. In sum, the invasion of E. adenophorum altered the diversity and structure of soil microbial communities, and changed the soil chemical properties.

Key words: plant invasion, Eupatorium adenophorum, high throughput sequencing, soil bacteria, biodiversity

Table 1

The chemical properties of soil in different sites. E, D, R represent samples from native plant community, Eupatorium adenophorum and native plant mixed community, and E. adenophorum dominated community. Values (mean ± SE) followed by different letters in the same column indicate significant difference at P <0.05 level."

样品
Sample
pH 全N
Total N (%)
有机质
Organic matter (%)
全P
Total P (mg/kg)
全K
Total K (%)
E 6.03±0.19a 0.337±0.131a 7.93±3.06a 1,256.75±249.93a 1.76±0.08a
D 5.67±0.26ac 0.256±0.026a 6.58±0.86a 747.00±24.76a 1.42±0.13b
R 5.18±0.10bc 0.250±0.069a 5.25±1.60a 5,659.00±615.13b 0.62±0.08c

Fig. 1

Alpha diversity for different samples obtained at genetic distances of 0.03. The letters E, D, R represent samples from native plant community, Eupatorium adenophorum and native plants mixed habitat, and E. adenophorum dominated habitat."

Fig. 2

The rarefaction of different samples obtained at the genetic distances of 0.03"

Fig. 3

Composition of the bacterial community at different taxonomic levels. (A) Phylum; (B) Order; (C) Genus. In (C), different colors represent relative abundances of bacteria. D1-D4 are soils from Eupatorium adenophorum and native plant mixed community; E1-E4 are soils from native plant community; R1-R4 are from communities dominated by E. adenophorum."

Fig. 4

Principal Component Analysis (PCA) of all microbial communities base on OTU data"

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