Biodiversity Science ›› 2015, Vol. 23 ›› Issue (1): 68-78.doi: 10.17520/biods.2014148

• Original Papers: Microbial Diversity • Previous Article     Next Article

Influence of long-term fertilization on the community structure and diversity of soybean rhizobia in black soil

Dawei Guan1, 2, Li Li1, 2, Xin Jiang1, 2, Mingchao Ma1, 2, Fengming Cao1, 2, Baoku Zhou3, Jun Li1, 2*   

  1. 1Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081

    2Laboratory of Quality & Safety Risk Assessment for Microbial Products, Ministry of Agriculture, Beijing 100081

    3Institute of Soil Fertilizer and Environment Resources, Heilongjiang Academy of Agricultural Sciences, Harbin 150006
  • Received:2014-07-10 Revised:2014-12-18 Online:2015-05-04
  • Jun Li E-mail:jli@caas.ac.cn

The objectives of this study were to explore the effects of long-term fertilization on the community structure and diversity of soybean rhizobia in black soil. BOX-PCR, IGS-PCR-RFLP and 16S rDNA gene sequencing methods were used to analyze the genetic diversity of the 254 soybean rhizobia isolated from 7 treatments at Heilongjiang Long-term Fertilization Experiment Station. The 7 treatments were as follows: no fertilization (CK), organic manure (OM), chemical nitrogen (N1), double chemical nitrogen (N2), chemical nitrogen-phosphorus (N1P1), double chemical nitrogen-phosphorus (N2P2), chemical nitrogen plus organic manure (N1+OM). Soil properties were measured and analyzed to test for relationships with the community and diversity of soybean rhizobia. All of the tested rhizobia belonged to Bradyrhizobium, of which the majority were closely related to B. japonicum and the others closely to B. liaoningense. BOX-PCR fingerprints showed that the tested rhizobia could be divided into 15 groups at 70% similarity level. These groups clustered into 3 communities that correspond to no chemical fertilizer treatments (CK, OM), chemical nitrogen fertilizer treatments (N1, N2, N1+OM), and chemical nitrogen-phosphorus treatments (N1P1, N2P2), respectively. Canonical correspondence analysis showed that pH, available N, and available P of the soil were significantly correlated with the community of rhizobia (P=0.002, 0.004, 0.002, respectively). Soybean rhizobia diversity indices varied significantly among fertilization treatments. Shannon-Wiener and species richness index were highest in N2P2 and Simpson index was highest in the OM treatment. The species richness, Shannon-Wiener and Simpson index in N1 and N2 were significantly lower than those in other treatments. The results of path analysis showed that the pH and available P had the greatest direct positive effects on the 3 indices, and that available N indirectly affected the 3 indices through pH. In conclusion, long-term chemical fertilization results in changes to community structure of soybean rhizobia in black soil, and application of chemical nitrogen fertilizer by itself reduces the diversity of soybean rhizobia, while application of nitrogenphosphorus has the opposite effect.

Key words: diversity indices, BOX-PCR, IGS-PCR-RFLP, canonical correspondence analysis, path analysis

[1] Xing Yuan, Wu Xiaoping, Ouyang Shan, Zhang Junqian, Xu Jing, Yin Senlu, Xie Zhicai. Assessment of macrobenthos biodiversity and potential human-induced stressors in the Ganjiang River system [J]. Biodiv Sci, 2019, 27(6): 648-657.
[2] Nuonan Ye, Naping Shen, Tianqi Shang, Hongdi Gao, Jieran Guan, Lita Yi. Vegetation Structure and Internal Relationship Between Distribution Patterns of Vegetation and Environment in Ecological Service Forest of Rui’an City in Zhejiang Province [J]. Chin Bull Bot, 2017, 52(4): 496-510.
[3] Youyin Ye,Peng Xiang,Yu Wang,Mao Lin. Phytoplankton diversity and its relationship with currents in the six bays of Fujian [J]. Biodiv Sci, 2017, 25(3): 285-293.
[4] Ye Liu,Peng Li,Yue Xu,Songlin Shi,Lingxiao Ying,Wanjun Zhang,Peihao Peng,Zehao Shen. Quantitative classification and ordination for plant communities in dry valleys of Southwest China [J]. Biodiv Sci, 2016, 24(4): 378-388.
[5] TAN Li-Ping,LIU Su-Xia,MO Xing-Guo,YANG Li-Hu,LIN Zhong-Hui. Environmental controls over energy, water and carbon fluxes in a plantation in Northern China [J]. Chin J Plan Ecolo, 2015, 39(8): 773-784.
[6] CAO Jing,MIAO Yan-Ming,FENG Fei,XU Qiang,ZHANG Qin-Di,BI Run-Cheng. Comparison of different treatments of rare species in canonical correspondence analysis [J]. Chin J Plan Ecolo, 2015, 39(2): 167-175.
[7] CHAI Yi, PENG Ting, GUO Kun, HE Yong-Feng, YANG De-Guo, and LUO Jing-Bo. Community characteristics of phytoplankton in Lake Changhu and relationships with environmental factors in the summer of 2012 [J]. Chin J Plan Ecolo, 2014, 38(8): 857-867.
[8] Chunyan Pi,Yan Liu. Bryophyte composition and diversity within anthropogenic habitats in a residential area of Chongqing municipality city [J]. Biodiv Sci, 2014, 22(5): 583-588.
[9] Jia Hang,Yun Shi,Wenhui Liu,Dahan He. Diversity of ground-dwelling beetles (Coleoptera) in restored habitats in the hill and gully area of Loess Plateau, Ningxia Hui Autonomous Region [J]. Biodiv Sci, 2014, 22(4): 516-524.
[10] YU Min, ZHOU Zhi-Yong, KANG Feng-Feng, OUYANG Shuai, MI Xiang-Cheng, and SUN Jian-Xin. Gradient analysis and environmental interpretation of understory herb-layer communities in Xiaoshegou of Lingkong Mountain, Shanxi, China [J]. Chin J Plan Ecolo, 2013, 37(5): 373-383.
[11] ZHU Jun-Tao, YU Jing-Jie, WANG Ping, WANG Zhi-Yong. Quantitative classification and analysis of relationships between plant communities and their groundwater environment in the Ejin Desert Oasis of China [J]. Chin J Plan Ecolo, 2011, 35(5): 480-489.
[12] Mo Gao, Renyong Hu, Xianxing Chen, Weicheng Li, Bingyang Ding. Effects of disturbance, topography, and soil conditions on the distribution of invasive plants in Wenzhou [J]. Biodiv Sci, 2011, 19(4): 424-431.
[13] Zengxiang Qi, Weihua Xu, Xingyao Xiong, Zhiyun Ouyang, Hua Zheng, Dexin Gan. Assessment of potential habitat for Ursus thibetanus in the Qinling Mountains [J]. Biodiv Sci, 2011, 19(3): 343-352.
[14] WANG You-Shi, CHU Cheng-Jin. A brief introduction of structural equation model and its application in ecology [J]. Chin J Plan Ecolo, 2011, 35(3): 337-344.
[15] SUN Ju, LI Xiu-Zhen, WANG Xian-Wei, Lü Jiu-Jun , LI Zong-Mei, HU Yuan-Man. Analysis of structures of permafrost wetland plant communities along environmental gradients in the Da Hinggan Mountains, China [J]. Chin J Plan Ecolo, 2010, 34(10): 1165-1173.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] . [J]. Plant Diversity, 2005, 27(02): 211 -216 .
[2] ZHANG Xiu-Jun, XU Hui, CHEN Guan-Xiong. N2O Emission Rate from Trees[J]. Chin J Plan Ecolo, 2002, 26(5): 538 -542 .
[3] Wang-Zhen GUO, Dong FANG, Wen-Duo YU and Tian-Zhen ZHANG. Sequence Divergence of Microsatellites and Phylogeny Analysis in Tetraploid Cotton Species and Their Putative Diploid Ancestors[J]. J Integr Plant Biol, 2005, 47(12): 1418 -1430 .
[4] Li Da Jue;Han Yun-zhou and Wan Li-ping. Studies on Germplasm Collections of Carthamus tinctorius IV Screening of the characterization of Seed Domancy[J]. Chin Bull Bot, 1990, 7(02): 50 -52 .
[5] Huanhuan Xu, Jian Kang, Mingxiang Liang. Research Advances in the Metabolism of Fructan in Plant Stress Resistance[J]. Chin Bull Bot, 2014, 49(2): 209 -220 .
[6] Lang Kai-Yung. Plantae Novae Aspidistrae Sinicae[J]. J Syst Evol, 1978, 16(1): 76 -77 .
[7] Naicheng Li, Xiaoshou Liu, Zhaodong Xu, Rui Zhao, Honghua Shi. Biodiversity of macrofauna in the southern waters of Miaodao Archipelago, China[J]. Biodiv Sci, 2015, 23(1): 41 -49 .
[8] Hong Ma, Kang Chong and Xing-Wang Deng. Rice Research: Past, Present and Future[J]. J Integr Plant Biol, 2007, 49(6): 729 -730 .
[9] Wang Kai-fa. Palynological Analysis Peat-bogs of Siyao Lake, Mount Hsishan, Nanchang, Kiangsi Province[J]. J Integr Plant Biol, 1974, 16(1): .
[10] YAN Kai, FU Deng-Gao, HE Feng, DUAN Chang-Qun. Leaf nutrient stoichiometry of plants in the phosphorus-enriched soils of the Lake Dianchi watershed, southwestern China[J]. Chin J Plan Ecolo, 2011, 35(4): 353 -361 .