生物多样性 ›› 1997, Vol. 05 ›› Issue (1): 14-25.  DOI: 10.17520/biods.1997003

• 论文 • 上一篇    下一篇

多角体蛋白的结构多样性和杆状病毒的进化研究*

刘子夜,齐义鹏,王家旺,黄永秀   

  1. (武汉大学病毒研究所,武汉 430072)
  • 收稿日期:1996-03-07 修回日期:1996-09-04 出版日期:1997-02-20 发布日期:1997-02-20

Structural comparisons of polyhedrin proteins and phylogeny of baculoviruses

Liu Ziye, Qi Yipeng, Wang jiawang, Huang Yongxiu   

  1. Institute of Virology,Wuhan University,Wuhan 430072
  • Received:1996-03-07 Revised:1996-09-04 Online:1997-02-20 Published:1997-02-20

摘要:

在测定LsMNPV多角体蛋白基因的全序列并推导出多角体蛋白氨基酸顺序的基础上,与22种杆状病毒多角体蛋白的氨基酸顺序进行比较,以氨基酸变异曲线研究蛋白质一级结构中氨基酸的保守性,并推测出一个模式多角体蛋白(MPh)氨基酸顺序;用PROSIS软件对MPh及其它5种代表性病毒的多角体蛋白进行了氨基酸亲水性分析,还对24种多角体蛋白的二级结构作出了推测,指出了特征性结构区与氨基酸高变区、亲水区多样性变化的相互关系;通过多角体蛋白间氨基酸顺序最大同源性分析,绘制了23种杆状病毒的系统进化树,进一步讨论了MPh氨基酸顺序的典型性及多角体蛋白所体现的杆状病毒多样性和宿主依赖性进化。

关键词: 西双版纳, 生境, 陆生软体动物, 物种多样性

Abstract

We have sequenced the polyhedrin gene from LsMNPV and the deduced amino acid sequence was compared with other polyhedrin or granulin amino acid sequences from 22 baculoviruses. Conservation of amino acid sequences was counted using a curve and a model polyhedrin (MPh) was given reasonably. Hydrophilicity of MPh and polyhedrins from some representative baculoviruses was studied by the PROSIS software. Prediction of secondary structure of MPh and 23 polyhedrins was stated in computer. Relationship among the predicted structure in special amino acid regions and the variability, the hydrophilicity was found. Evolution of 23 baculoviruses was discussed according to the maximum homologous analysis of amino acid sequences between two polyhedrin separately.

Key words: Xishuangbanna, habitats, terrestrial molluskes, species diversity