Biodiv Sci ›› 2013, Vol. 21 ›› Issue (3): 315-325.DOI: 10.3724/SP.J.1003.2013.11035
• Orginal Article • Previous Articles Next Articles
Zhouhe Du1,2, Junfeng Liu1,*, Binbin Liu1, Yanchun Zuo1, Jianmei Wu1, Yi-an Chen1, Jianfei Zhang1, Cheng Lu2,*()
Received:
2013-02-04
Accepted:
2013-04-26
Online:
2013-05-20
Published:
2013-06-05
Contact:
Liu Junfeng,Lu Cheng
Zhouhe Du,Junfeng Liu,Binbin Liu,Yanchun Zuo,Jianmei Wu,Yi-an Chen,Jianfei Zhang,Cheng Lu. Genetic diversity and molecular genealogy of local silkworm varieties[J]. Biodiv Sci, 2013, 21(3): 315-325.
序号 No. | 品种 Variety | 代号 Code | 生态类型 Ecotype | 序号 No. | 品种 Variety | 代号 Code | 生态类型 Ecotype |
---|---|---|---|---|---|---|---|
1 | 小石丸 Xiaoshiwan | XSW | CV1 | 37 | 萨尼斯 Sanisi | SNS | EV1 |
2 | 二毛 Ermao | EM | CV1 | 38 | 罗尼7 Luoni7 | LN7 | EV1 |
3 | 土三虎斑 Tusanhuban | TSHB | CV1 | 39 | 法408 Fa408 | F408 | EV1 |
4 | 中土 Zhongtu | ZT | CV1 | 40 | 欧18 Ou18 | O18 | EV1 |
5 | 达县土种 Daxiantuzhong | DXTZ | CV1 | 41 | 意16 Yi16 | Y16 | EV1 |
6 | 土一肉黄圆 Tuyirouhuangyuan | TYRHY | CV1 | 42 | 乌D WuD | WD | EV2 |
7 | 土八 Tuba | Tba | CV1 | 43 | 乌G WuG | WG | EV2 |
8 | 鲁1 Lu1 | L1 | CV1 | 44 | 乌B WuB | WB | EV2 |
9 | Re黑 Rehei | RH | CV1 | 45 | 乌2 Wu2 | W2 | EV2 |
10 | 鲁10 Lu10 | L10 | CV1 | 46 | 日110 Ri110 | R110 | T |
11 | 土种01 Tuzhong01 | TZ01 | CV1 | 47 | 大造 Dazao | DZ | T |
12 | 诸桂 Zhugui | ZG | CV1 | 48 | 高花 Gaohua | GH | T |
13 | 温州种 Wenzhouzhong | WZZ | CV1 | 49 | 第五白卵 Diwubailuan | DWBL | T |
14 | 芙蓉灰卵 Furonghuiluan | FRHL | CV1 | 50 | 演金黄白 Yanjinhuangbai | YJHB | T |
15 | 灰色卵 Huiseluan | HSL | CV1 | 51 | 15-100 | 15-100 | T |
16 | 盱眙种 Xuyizhong | XYZ | CV1 | 52 | 07-310 | 07-310 | T |
17 | 余杭24 Yuhang24 | YH24 | CV1 | 53 | 高白 Gaobai | GB | T |
18 | 沛县种 Peixianzhong | PXZ | CV1 | 54 | 琼山海南 Qiongshanhainan | QSHN | T |
19 | 邯郸种 Handanzhong | HDZ | CV1 | 55 | 655 | 655 | T |
20 | 中4010 Zhong4010 | Z4010 | CV2 | 56 | 防四 Fangsi | FS | T |
21 | 泰 Tai | T | CV2 | 57 | 印度种 Yinduzhong | YDZ | T |
22 | 玉溪2号 Yuxi2hao | YX | CV2 | 58 | 加秋 Jiaqiu | JQ | T |
23 | 平 Ping | P | CV2 | 59 | 白夏B BaixiaB | BXB | T |
24 | C108 | C108 | CV2 | 60 | 天龙青白 Tianlongqingbai | TLQB | JV1 |
25 | 余杭2 Yuhang2 | YH2 | CV2 | 61 | 赤熟 Chishu | Cshu | JV1 |
26 | 土白 Tubai | Tbai | CV2 | 62 | 日9 Ri9 | R9 | JV1 |
27 | 松花形吴 Songhuaxingwu | SHXW | CV2 | 63 | 姬蚕 Jican | JC | JV1 |
28 | 保黄 Baohuang | BH | CVd | 64 | 黑子 Heizi | HZ | JV1 |
29 | MCH101 | MCH101 | CVd | 65 | 日10 Ri10 | R10 | JV1 |
30 | BT924 | BT924 | CVd | 66 | 绵蚕 Miancan | MC | JV1 |
31 | BH863 | BH863 | CVd | 67 | 日限2 Rixian2 | RX2 | JV2 |
表1 (续) Table 1 (continued) | |||||||
序号 No. | 品种 Variety | 代号 Code | 生态类型 Ecotype | 序号 No. | 品种 Variety | 代号 Code | 生态类型 Ecotype |
32 | 琼十 Qiongshi | QS | CVd | 68 | 赢纹形 Yingwenxing | YWX | JV2 |
33 | 四海 Sihai | SH | CVd | 69 | J115 | J115 | JV2 |
34 | 兰十 Lanshi | LanS | CVd | 70 | 830 | 830 | JV2 |
35 | 罗萨 Luosa | LuoS | EV1 | 71 | 春四 Chunsi | CS | JV2 |
36 | 阿利可斯 Alikesi | ALKS | EV1 | 72 | T8 | T8 | JV2 |
Table 1 The origin and characters of 72 silkworm varieties used in this study
序号 No. | 品种 Variety | 代号 Code | 生态类型 Ecotype | 序号 No. | 品种 Variety | 代号 Code | 生态类型 Ecotype |
---|---|---|---|---|---|---|---|
1 | 小石丸 Xiaoshiwan | XSW | CV1 | 37 | 萨尼斯 Sanisi | SNS | EV1 |
2 | 二毛 Ermao | EM | CV1 | 38 | 罗尼7 Luoni7 | LN7 | EV1 |
3 | 土三虎斑 Tusanhuban | TSHB | CV1 | 39 | 法408 Fa408 | F408 | EV1 |
4 | 中土 Zhongtu | ZT | CV1 | 40 | 欧18 Ou18 | O18 | EV1 |
5 | 达县土种 Daxiantuzhong | DXTZ | CV1 | 41 | 意16 Yi16 | Y16 | EV1 |
6 | 土一肉黄圆 Tuyirouhuangyuan | TYRHY | CV1 | 42 | 乌D WuD | WD | EV2 |
7 | 土八 Tuba | Tba | CV1 | 43 | 乌G WuG | WG | EV2 |
8 | 鲁1 Lu1 | L1 | CV1 | 44 | 乌B WuB | WB | EV2 |
9 | Re黑 Rehei | RH | CV1 | 45 | 乌2 Wu2 | W2 | EV2 |
10 | 鲁10 Lu10 | L10 | CV1 | 46 | 日110 Ri110 | R110 | T |
11 | 土种01 Tuzhong01 | TZ01 | CV1 | 47 | 大造 Dazao | DZ | T |
12 | 诸桂 Zhugui | ZG | CV1 | 48 | 高花 Gaohua | GH | T |
13 | 温州种 Wenzhouzhong | WZZ | CV1 | 49 | 第五白卵 Diwubailuan | DWBL | T |
14 | 芙蓉灰卵 Furonghuiluan | FRHL | CV1 | 50 | 演金黄白 Yanjinhuangbai | YJHB | T |
15 | 灰色卵 Huiseluan | HSL | CV1 | 51 | 15-100 | 15-100 | T |
16 | 盱眙种 Xuyizhong | XYZ | CV1 | 52 | 07-310 | 07-310 | T |
17 | 余杭24 Yuhang24 | YH24 | CV1 | 53 | 高白 Gaobai | GB | T |
18 | 沛县种 Peixianzhong | PXZ | CV1 | 54 | 琼山海南 Qiongshanhainan | QSHN | T |
19 | 邯郸种 Handanzhong | HDZ | CV1 | 55 | 655 | 655 | T |
20 | 中4010 Zhong4010 | Z4010 | CV2 | 56 | 防四 Fangsi | FS | T |
21 | 泰 Tai | T | CV2 | 57 | 印度种 Yinduzhong | YDZ | T |
22 | 玉溪2号 Yuxi2hao | YX | CV2 | 58 | 加秋 Jiaqiu | JQ | T |
23 | 平 Ping | P | CV2 | 59 | 白夏B BaixiaB | BXB | T |
24 | C108 | C108 | CV2 | 60 | 天龙青白 Tianlongqingbai | TLQB | JV1 |
25 | 余杭2 Yuhang2 | YH2 | CV2 | 61 | 赤熟 Chishu | Cshu | JV1 |
26 | 土白 Tubai | Tbai | CV2 | 62 | 日9 Ri9 | R9 | JV1 |
27 | 松花形吴 Songhuaxingwu | SHXW | CV2 | 63 | 姬蚕 Jican | JC | JV1 |
28 | 保黄 Baohuang | BH | CVd | 64 | 黑子 Heizi | HZ | JV1 |
29 | MCH101 | MCH101 | CVd | 65 | 日10 Ri10 | R10 | JV1 |
30 | BT924 | BT924 | CVd | 66 | 绵蚕 Miancan | MC | JV1 |
31 | BH863 | BH863 | CVd | 67 | 日限2 Rixian2 | RX2 | JV2 |
表1 (续) Table 1 (continued) | |||||||
序号 No. | 品种 Variety | 代号 Code | 生态类型 Ecotype | 序号 No. | 品种 Variety | 代号 Code | 生态类型 Ecotype |
32 | 琼十 Qiongshi | QS | CVd | 68 | 赢纹形 Yingwenxing | YWX | JV2 |
33 | 四海 Sihai | SH | CVd | 69 | J115 | J115 | JV2 |
34 | 兰十 Lanshi | LanS | CVd | 70 | 830 | 830 | JV2 |
35 | 罗萨 Luosa | LuoS | EV1 | 71 | 春四 Chunsi | CS | JV2 |
36 | 阿利可斯 Alikesi | ALKS | EV1 | 72 | T8 | T8 | JV2 |
序列长度 Sequence length (bp) | 碱基插入/缺失位点数 Insert/delete (%) | 变异位点数 Variable site (%) | 单个突变位点 Singleton variable site (%) | 简约信息位点 Parsimony variable site (%) | |
---|---|---|---|---|---|
外显子 Exon | 493 | 2(0.4)* | 10(2.0) | 2(0.4) | 8(1.6) |
内含子 Intron | 670 | 139(20.7) | 55(8.2) | 7(1.0) | 48(7.2) |
全序列 Entire | 1,163 | 141(12.1) | 65(5.6) | 9(0.8) | 56(4.8) |
Table 2 Statistics of the varieties bases of 72 local silkworm varieties in the Bmamy2 gene amplified region
序列长度 Sequence length (bp) | 碱基插入/缺失位点数 Insert/delete (%) | 变异位点数 Variable site (%) | 单个突变位点 Singleton variable site (%) | 简约信息位点 Parsimony variable site (%) | |
---|---|---|---|---|---|
外显子 Exon | 493 | 2(0.4)* | 10(2.0) | 2(0.4) | 8(1.6) |
内含子 Intron | 670 | 139(20.7) | 55(8.2) | 7(1.0) | 48(7.2) |
全序列 Entire | 1,163 | 141(12.1) | 65(5.6) | 9(0.8) | 56(4.8) |
CV2 | EV1 | T | CV1M3 | JV2 | CV1M4 | JV1 | CVd | |
---|---|---|---|---|---|---|---|---|
CV2 | ||||||||
EV1 | 0.025 | |||||||
T | 0.027 | 0.024 | ||||||
CV1M3 | 0.026 | 0.015 | 0.023 | |||||
JV2 | 0.026 | 0.012 | 0.024 | 0.016 | ||||
CV1M4 | 0.026 | 0.013 | 0.024 | 0.017 | 0.014 | |||
JV1 | 0.027 | 0.025 | 0.024 | 0.025 | 0.026 | 0.025 | ||
CVd | 0.025 | 0.025 | 0.024 | 0.025 | 0.026 | 0.025 | 0.024 | |
EV2 | 0.025 | 0.044 | 0.029 | 0.039 | 0.044 | 0.042 | 0.026 | 0.026 |
Table 3 Genetic distance among different ecotypic varieties of local silkworm
CV2 | EV1 | T | CV1M3 | JV2 | CV1M4 | JV1 | CVd | |
---|---|---|---|---|---|---|---|---|
CV2 | ||||||||
EV1 | 0.025 | |||||||
T | 0.027 | 0.024 | ||||||
CV1M3 | 0.026 | 0.015 | 0.023 | |||||
JV2 | 0.026 | 0.012 | 0.024 | 0.016 | ||||
CV1M4 | 0.026 | 0.013 | 0.024 | 0.017 | 0.014 | |||
JV1 | 0.027 | 0.025 | 0.024 | 0.025 | 0.026 | 0.025 | ||
CVd | 0.025 | 0.025 | 0.024 | 0.025 | 0.026 | 0.025 | 0.024 | |
EV2 | 0.025 | 0.044 | 0.029 | 0.039 | 0.044 | 0.042 | 0.026 | 0.026 |
Fig. 4 Phylogenetic (NJ) tree based on nucleotide sequence of Bmamy2 gene of Bombyx mori. Nucleotide P-distance model and pairwise deletion of gaps were selected for the tree reconstruction in the program MEGA 4.0, and bootstrap values (1,000 replicate) of >40% are shown. Variety codes see Table 1.
聚类支 Clade | 地理生态类型 Ecotype | 品种数(个) No. of varieties | 占各支百分比 % |
---|---|---|---|
A | 中系一化 CV1 | 15 | 36.59 |
中系二化 CV2 | 4 | 9.76 | |
中系多化 CVd | 3 | 7.32 | |
日系一化 JV1 | 3 | 7.32 | |
日系二化 JV2 | 5 | 12.20 | |
欧系一化 EV1 | 6 | 14.63 | |
热带系统 T | 5 | 12.20 | |
小计 Sum | 41 | ||
B | 中系一化 CV1 | 6 | 33.33 |
中系多化 CVd | 2 | 11.11 | |
日系一化 JV1 | 2 | 11.11 | |
日系二化 JV2 | 1 | 5.56 | |
欧系一化 EV1 | 1 | 5.46 | |
热带系统 T | 6 | 33.33 | |
小计 Sum | 18 | ||
C | 中系二化 CV2 | 4 | 30.77 |
中系多化 CVd | 2 | 15.38 | |
日系一化 JV1 | 2 | 14.38 | |
欧系二化 EV2 | 4 | 30.77 | |
热带系统 T | 1 | 7.69 | |
小计 Sum | 13 | ||
合计 | Total | 72 |
Table 4 Silkworm varieties belonging to different clades
聚类支 Clade | 地理生态类型 Ecotype | 品种数(个) No. of varieties | 占各支百分比 % |
---|---|---|---|
A | 中系一化 CV1 | 15 | 36.59 |
中系二化 CV2 | 4 | 9.76 | |
中系多化 CVd | 3 | 7.32 | |
日系一化 JV1 | 3 | 7.32 | |
日系二化 JV2 | 5 | 12.20 | |
欧系一化 EV1 | 6 | 14.63 | |
热带系统 T | 5 | 12.20 | |
小计 Sum | 41 | ||
B | 中系一化 CV1 | 6 | 33.33 |
中系多化 CVd | 2 | 11.11 | |
日系一化 JV1 | 2 | 11.11 | |
日系二化 JV2 | 1 | 5.56 | |
欧系一化 EV1 | 1 | 5.46 | |
热带系统 T | 6 | 33.33 | |
小计 Sum | 18 | ||
C | 中系二化 CV2 | 4 | 30.77 |
中系多化 CVd | 2 | 15.38 | |
日系一化 JV1 | 2 | 14.38 | |
欧系二化 EV2 | 4 | 30.77 | |
热带系统 T | 1 | 7.69 | |
小计 Sum | 13 | ||
合计 | Total | 72 |
变异来源 Source of variance | 自由度 df | 方差分量 Variance component | 变异百分比 (%) | P-value |
---|---|---|---|---|
地理生态类型组群间 Among geo-voltings | 7 | 6.93028 | 19.86 | > 0.05 |
地理生态类型组群内 Among population within geo-voltings | 1 | 1.23267 | 3.53 | > 0.05 |
种群内 Within populations | 63 | 26.73978 | 76.61 | < 0.01 |
Table 5 AMOVA of Bmamy2 gene for 72 silkworm varieties of Bombyx mori based on geo-ecotype
变异来源 Source of variance | 自由度 df | 方差分量 Variance component | 变异百分比 (%) | P-value |
---|---|---|---|---|
地理生态类型组群间 Among geo-voltings | 7 | 6.93028 | 19.86 | > 0.05 |
地理生态类型组群内 Among population within geo-voltings | 1 | 1.23267 | 3.53 | > 0.05 |
种群内 Within populations | 63 | 26.73978 | 76.61 | < 0.01 |
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