Effect of soil factors on arbuscular mycorrhizal fungi in saline alkaline soils of Gansu, Inner Mongolia and Ningxia

doi:10.3724/SP.J.1003.2011.08145

Abstract

Abstract:

In order to explore abuscular mycorrhizal (AM) fungal resources and reveal the relationship between soil factors and AM fungal diversity, we collected soil samples from the rhizosphere of main plants in saline alkaline soils of Gansu, Inner Mongolia and Ningxia. A total of 28 AM fungal species were identified. Of these, 20 species belonged to the genus Glomus, 2 to Diversispora, 2 to Archaeospora, and 4 to Acaulospora. The frequencies of G. intraradices and Ar. leptotichum were the highest. Correlation analysis revealed that AM fungal species richness was significantly correlated with the contents of soil organic matter and available N, while Shannon-Wiener diversity index was significantly related with soil organic matter content. Path analysis revealed that organic matter had an significantly direct negative influence on Shannon-Wiener diversity index; Cl^-, CO₃^2-, K⁺, Mg²⁺, organic matter, available P and available K directly impacted Shannon-Wiener diversity index, while HCO₃^-, Na⁺, Ca²⁺, SO₄^2-, soluble salt, pH value and available N indirectly affected Shannon-Wiener diversity index; Cl^-, CO₃^2-, HCO₃^-, Na⁺, Ca²⁺, organic matter, available P and available K directly impacted AM fungal species richness, while SO₄^2-, K⁺, soluble salt, Mg²⁺, pH value and available N indirectly affected AM fungal species richness. Redundancy analysis revealed that the relative abundance of 14 AM fungal species was significantly correlated with soil factors. Our results indicate that there are plenty of AM fungal species in the saline alkaline soils of the three regions, and AM fungal diversity is related with nutrient status and contents of soluble saline and ions in soils.

Key words: saline alkaline soil, soil factor, arbuscular mycorrhizal fungi, species richness, relative abundance, Shannon-Wiener diversity index

Min Sheng, Ming Tang, Fengfeng Zhang, Yanhui Huang. Effect of soil factors on arbuscular mycorrhizal fungi in saline alkaline soils of Gansu, Inner Mongolia and Ningxia[J]. Biodiv Sci, 2011, 19(1): 85-92.

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URL: https://www.biodiversity-science.net/EN/10.3724/SP.J.1003.2011.08145

https://www.biodiversity-science.net/EN/Y2011/V19/I1/85

Figures/Tables 6

References 27

[1]	Agwa HE, Abdel-Fattah GM (2002) Arbuscular mycorrhizal fungi (Glomales) in Egypt. II. An ecological view of some saline affected plants in the deltaic Mediterranean coastal land. Acta Botanica Hungarica, 44,1-17. DOI URL
[2]	Aliasgharzadeh N, Saleh Rastin N, Towfighi H, Alizadeh A (2001) Occurrence of arbuscular mycorrhizal fungi in saline soils of the Tabriz Plain of Iran in relation to some physical and chemical properties of soil. Mycorrhiza, 11,119-122. URL PMID
[3]	Bao SD (鲍士旦) (2000) Soil Agricultural Chemical Analysis (土壤农化分析). China Agricultural Press, Beijing. (in Chinese)
[4]	Gai JP (盖京苹), Feng G (冯固), Li XL (李晓林) (2004) Diversity of arbuscular mycorrhizal fungi in field soils from north China. Biodiversity Science (生物多样性), 12,435-440. (in Chinese with English abstract)
[5]	Hildebrandt U, Janetta K, Ouziad F, Renne B, Nawrath K, Bothe H (2001) Arbuscular mycorrhizal colonization of halophytes in Central European salt marshes. Mycorrhiza, 10,175-183. DOI URL
[6]	Hu XP (胡小平), Wang CF (王长发) (2001) SAS Basis and Statistical Examples (SAS基础及统计实例教程). Xi’an Cartographic Publishing House, Xi’an. (in Chinese)
[7]	Joner EJ, Jakobsen I (1995) Growth and extracellular phosphatase activity of arbuscular mycorrhizal hyphae as influenced by soil organic matter. Soil Biology and Biochemistry, 27,1153-1159.
[8]	Juniper S, Abbott LK (2006) Soil salinity delays germination and limits growth of hyphae from propagules of arbuscular mycorrhizal fungi. Mycorrhiza, 16,371-379. URL PMID
[9]	Koske RE, Walker C (1984) Gigaspora erythropa, a new species forming arbuscular mycorrhizae. Mycologia, 76,250-255.
[10]	Liu RJ (刘润进), Li XL (李晓林) (2000) Arbuscular Mycorrhizae and Its Applications (丛枝菌根及其应用). Science Press, Beijing. (in Chinese)
[11]	Liu RJ (刘润进), Liu PQ (刘鹏起), Xu K (徐坤), Lü ZF (吕志范) (1999) Ecological distribution of arbuscular mycorrhizal fungi in saline soils of China. Chinese Journal of Applied Ecology (应用生态学报), 10,721-724. (in Chinese with English abstract)
[12]	Maas EV, Grieve CM (1987) Sodium-induced calcium deficiency in salt-stressed corn. Plant, Cell and Environment, 10,559-564.
[13]	Oliveira RS, Vosatka M, Dodd JC, Castro PM (2005) Studies on the diversity of arbuscular mycorrhizal fungi and the efficacy of two native isolates in a highly alkaline anthropogenic sediment. Mycorrhiza, 16,23-31. URL PMID
[14]	Schenck NC, Perez Y (1988) Manual for the Identification of Vesicular Arbuscular Mycorrhizal Fungi, 2nd edn. pp.1-233. S ynergistic Publications, Gaineville, Florida.
[15]	Shannon CE, Weaver W (1963) The Mathematical Theory of Communication. University of Illinois Press, Urbana and Chicago.
[16]	Sheng M (盛敏), Tang M (唐明), Zhang FF (张峰峰), Huang YH (黄艳辉), Yang BW (杨保伟) (2008) Effect of soil factors on arbuscular mycorrhizal fungi in northwest saline alkaline soil. Acta Pedologica Sinica (土壤学报), 45,758-763. (in Chinese with English abstract)
[17]	Sheng M, Tang M, Chen H, Yang BW, Zhang FF, Huang YH (2009) Influence of arbuscular mycorrhizae on root system of maize plants under salt stress. Canadian Journal of Microbiology, 55,879-886. URL PMID
[18]	Shi YL (石玉林) (1991) 1: 1000000 Map of Chinese land Resource (《中国1 : 100 万土地资源图》土地资源数据集) China Renmin University Press, Beijing. (in Chinese)
[19]	Tang M (唐明), Huang YH (黄艳辉), Sheng M (盛敏), Zhang FF (张峰峰), Xiao WF (肖文发) (2007) Diversity and distribution of arbuscular mycorrhizal fungi in saline alkaline soil, Inner Mongolia. Acta Pedologica Sinica (土壤学报), 44,1104-1110. (in Chinese with English abstract)
[20]	Tawaraya K, Saito M, Morioka M, Waqatsuma T (1994) Effect of phosphate application to arbuscular mycorrhizal onion on the development and succinate dehydrogenase activity of internal hyphae. Soil Science and Plant Nutrition, 40,667-673.
[21]	Tawaraya K, Watanabe S, Yoshida E, Waqatsuma T (1996) Effect of onion (Allium cepa) root exudates on the hyphal growth of Gigaspora margarita. Mycorrhiza, 6,57-59.
[22]	van Hoorn JW, Katerji N, Hamdy A, Mastrorilli M (2001) Effect of salinity on yield and nitrogen uptake of four grain legumes and on biological nitrogen contribution from the soil. Agricultural Water Management, 51,87-98.
[23]	Wang FY, Liu RJ, Lin XG, Zhou JM (2004) Arbuscular mycorrhizal status of wild plants in saline-alkaline soils of the Yellow River Delta. Mycorrhiza, 14,133-137. URL PMID
[24]	Wang FY (王发园), Liu RJ (刘润进) (2001) A preliminary survey of arbuscular mycorrhizal fungi in saline-alkaline soil of the Yellow River Delta. Biodiversity Science (生物多样性), 9,389-392. (in Chinese with English abstract)
[25]	Wilde P, Manal A, Stodden M, Ewald S, Ulrich H, Hermann B (2009) Biodiversity of arbuscular mycorrhizal fungi in roots and soils of two salt marshes. Environmental Microbiology, 11,1548-1561. DOI URL PMID
[26]	Walker C, Schüßler A (2004) Nomenclatural clarifications and new taxa in the Glomeromycota. Mycological Research, 108,981-982.
[27]	Yano-Melo AM, Saggin OJ Jr, Maia LC (2003) Tolerance of mycorrhized banana ( Musa sp. cv. Pacovan) plantlets to saline stress. Agriculture, Ecosystems and Environment, 95,343-348.

采样地点 Sampling site	植物种类 Plant species	海拔 Elevation (m)	经纬度 Locality
甘肃安西 Anxi County, Gansu	苜蓿(Medicago sativa)、小麦(Triticum aestivum)	1,135	40°29′N, 95°36′E
甘肃敦煌 Dunhuang County, Gansu	棉花(Gossypium herbaceum)、骆驼刺(Alhagi sparsifolia)	1,050	40°25′N, 94°41′E
甘肃玉门镇 Yumen Town, Gansu	沙枣(Elaegnus angustifolia)、马蔺(Iris lactea)、芨芨草(Achnatherum splendens)、甘草(Glycyrrhiza uralensis)	1,404	40°26′N, 97°00′E
宁夏平罗 Pingluo County, Ningxia	玉米(Zea mays)、葱(Allium fistulosum)、胡杨(Populus euphratica)、椿树(Ailanthus altissima)、砂柳(Salix gordejevii)、水稻(Oryza sativa)、冰草(Agropyron cristatum)、枸杞(Lycium chinense)、大米草(Spartina anglica)、芨芨草、白刺(Nitraria tangutorum)	1,099	38°48′N, 106°16′E
内蒙古呼和浩特市托克托县 Tuoketok County, Inner Mongolia	白刺、芦苇(Phragmites australis)	986	40°17′N, 111°09′E
内蒙古哲里木盟奈曼旗 Naiman Banner, Zhelimu League, Inner Mongolia	寸草苔(Carex duriuscula)、冰草、盐角草(Saliconia europaea)、芦苇、马蔺、柠条(Caragana korshinkii)
内蒙古科尔沁左翼中旗 Ke′erqin Left Middle Banner, Inner Mongolia	向日葵(Helianthus annnus)、羊草(Leymus chinensis)、筛草(Carex kobomugi)	259	45°3′N, 121°28′E

采样地点 Sampling site	植物种类 Plant species	海拔 Elevation (m)	经纬度 Locality
甘肃安西 Anxi County, Gansu	苜蓿(Medicago sativa)、小麦(Triticum aestivum)	1,135	40°29′N, 95°36′E
甘肃敦煌 Dunhuang County, Gansu	棉花(Gossypium herbaceum)、骆驼刺(Alhagi sparsifolia)	1,050	40°25′N, 94°41′E
甘肃玉门镇 Yumen Town, Gansu	沙枣(Elaegnus angustifolia)、马蔺(Iris lactea)、芨芨草(Achnatherum splendens)、甘草(Glycyrrhiza uralensis)	1,404	40°26′N, 97°00′E
宁夏平罗 Pingluo County, Ningxia	玉米(Zea mays)、葱(Allium fistulosum)、胡杨(Populus euphratica)、椿树(Ailanthus altissima)、砂柳(Salix gordejevii)、水稻(Oryza sativa)、冰草(Agropyron cristatum)、枸杞(Lycium chinense)、大米草(Spartina anglica)、芨芨草、白刺(Nitraria tangutorum)	1,099	38°48′N, 106°16′E
内蒙古呼和浩特市托克托县 Tuoketok County, Inner Mongolia	白刺、芦苇(Phragmites australis)	986	40°17′N, 111°09′E
内蒙古哲里木盟奈曼旗 Naiman Banner, Zhelimu League, Inner Mongolia	寸草苔(Carex duriuscula)、冰草、盐角草(Saliconia europaea)、芦苇、马蔺、柠条(Caragana korshinkii)
内蒙古科尔沁左翼中旗 Ke′erqin Left Middle Banner, Inner Mongolia	向日葵(Helianthus annnus)、羊草(Leymus chinensis)、筛草(Carex kobomugi)	259	45°3′N, 121°28′E

AM真菌种属 Genera and species of AM fungi	分离频度 Frequency (%)	AM真菌种属 Genera and species of AM fungi	分离频度 Frequency (%)
无梗囊霉属 Acaulospora	36.7	明球囊霉 G. clarum	6.7
浅窝无梗囊霉 A. lacunosa	3.3	缩球囊霉 G. constrictum	66.7
皱壁无梗囊霉 A. rugosa	6.7	副冠球囊霉 G. coronatum	26.7
细凹无梗囊霉 A. scrobiculata	16.7	透光球囊霉 G. diaphanum	3.3
刺无梗囊霉 A. spinosa	16.7	聚生球囊霉 G. fasciculatum	10.0
原囊霉属 Archaeospora	83.3	地球囊霉 G. geosporum	76.7
格氏原囊霉 Ar. gerdemannii	6.7	根内球囊霉 G. intraradices	80.0
薄壁原囊霉 Ar. leptotichum	80.0	木薯球囊霉 G. manihotis	23.3
多孢囊霉属 Diversispora	76.7	摩西球囊霉 G. mosseae	33.3
幼套多孢囊霉 D. etunicatum	3.3	膨果球囊霉 G. pansihalos	3.3
地表多孢囊霉 D. versiforme	73.3	具疱球囊霉 G. pustulatum	16.7
球囊霉属 Glomus	96.7	网状球囊霉 G. reticulatum	3.3
聚丛球囊霉 G. aggregatum	6.7	荫性球囊霉 G. tenebrosum	43.3
白色球囊霉 G. albidum	3.3	三壁球囊霉 G. trimurales	3.3
澳洲球囊霉 G. australe	26.7	疣壁球囊霉 G. verruculosum	20.0
近明球囊霉 G. claroideum	13.3	泡囊球囊霉 G. vesiculiferum	3.3

AM真菌种属 Genera and species of AM fungi	分离频度 Frequency (%)	AM真菌种属 Genera and species of AM fungi	分离频度 Frequency (%)
无梗囊霉属 Acaulospora	36.7	明球囊霉 G. clarum	6.7
浅窝无梗囊霉 A. lacunosa	3.3	缩球囊霉 G. constrictum	66.7
皱壁无梗囊霉 A. rugosa	6.7	副冠球囊霉 G. coronatum	26.7
细凹无梗囊霉 A. scrobiculata	16.7	透光球囊霉 G. diaphanum	3.3
刺无梗囊霉 A. spinosa	16.7	聚生球囊霉 G. fasciculatum	10.0
原囊霉属 Archaeospora	83.3	地球囊霉 G. geosporum	76.7
格氏原囊霉 Ar. gerdemannii	6.7	根内球囊霉 G. intraradices	80.0
薄壁原囊霉 Ar. leptotichum	80.0	木薯球囊霉 G. manihotis	23.3
多孢囊霉属 Diversispora	76.7	摩西球囊霉 G. mosseae	33.3
幼套多孢囊霉 D. etunicatum	3.3	膨果球囊霉 G. pansihalos	3.3
地表多孢囊霉 D. versiforme	73.3	具疱球囊霉 G. pustulatum	16.7
球囊霉属 Glomus	96.7	网状球囊霉 G. reticulatum	3.3
聚丛球囊霉 G. aggregatum	6.7	荫性球囊霉 G. tenebrosum	43.3
白色球囊霉 G. albidum	3.3	三壁球囊霉 G. trimurales	3.3
澳洲球囊霉 G. australe	26.7	疣壁球囊霉 G. verruculosum	20.0
近明球囊霉 G. claroideum	13.3	泡囊球囊霉 G. vesiculiferum	3.3

	Cl^-	SO₄^2-	CO₃^2-	HCO₃^-	Na⁺	K⁺	Ca²⁺	Mg²⁺	水溶性全盐 Soluble salt	pH	有机质 Organic matter	速效P Available P	速效K Available K	速效N Available N
SR	-0.36	0.16	0.23	0.17	-0.210	0.0084	-0.0055	-0.082	0.071	0.22	-0.55^*	-0.14	0.021	-0.44^*
H	-0.26	0.21	0.11	0.15	-0.092	0.069	0.058	0.0410	0.130	0.14	-0.48^*	-0.29	0.048	-0.32