短吻红舌鳎鱼卵、仔鱼的DNA条形码和形态学鉴定

doi:10.17520/biods.2017094

生物多样性 ›› 2017, Vol. 25 ›› Issue (8): 847-855. DOI: 10.17520/biods.2017094

• 研究报告: 动物多样性 • 上一篇下一篇

短吻红舌鳎鱼卵、仔鱼的DNA条形码和形态学鉴定

周晓梦¹, 郭书新¹, 宋娜¹, 张秀梅^1,^2,^*()

1 (中国海洋大学海水养殖教育部重点实验室, 山东青岛 266003)
2 (青岛海洋科学与技术国家实验室, 海洋渔业科学与食物产出过程功能实验室, 山东青岛 266072)

收稿日期:2017-03-23 接受日期:2017-06-19 出版日期:2017-08-20 发布日期:2017-08-31
通讯作者: 张秀梅
作者简介:# 共同第一作者 Co-first authors
基金资助:
国家自然科学基金(31572227, 41676153);青岛海洋科学与技术国家实验室鳌山科技创新计划项目(2015ASKJ01)

Identification of Cynoglossus joyneri eggs and larvae by DNA barcoding and morphological method

Xiaomeng Zhou¹, Shuxin Guo¹, Na Song¹, Xiumei Zhang^1,^2,^*()

1 Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, Shandong 266003
2 Functional Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong 266072

Received:2017-03-23 Accepted:2017-06-19 Online:2017-08-20 Published:2017-08-31
Contact: Zhang Xiumei

摘要/Abstract

摘要：

为了准确鉴定2015年7-10月青岛崂山青山湾鱼类浮游生物调查中采集的依形态学初步鉴定为某种舌鳎属的鱼卵、仔鱼样本, 本实验同时采集青岛胶州湾短吻红舌鳎(Cynoglossus joyneri)和长吻红舌鳎(C. lighti)的成鱼样本为参照, 通过线粒体COI基因测序分析, 以及同山东半岛海域分布的6种舌鳎属鱼类的COI基因序列比对, 开展了该舌鳎属鱼卵、仔鱼种类的鉴别分析, 以期为今后舌鳎属鱼类的分类及其鱼卵、仔鱼的鉴别提供参考资料。形态学研究结果显示: 鱼卵为圆球形浮性卵, 直径0.68-0.87 mm (0.73 ± 0.03 mm, n = 50), 卵膜单层, 较薄、光滑透明, 卵周隙较大, 卵膜及卵黄囊均无特殊构造, 油球6-15个, 直径0.04-0.10 mm (0.07 ± 0.01 mm, n = 50), 圆形, 大小不一, 多位于卵黄囊中与胚体相对的一侧, 既有聚集分布也有分散分布; 仔鱼的2条冠状幼鳍及右眼移位过程中体长、色素分布等的特征和变化是此鱼种仔鱼鉴别的最明显特征。遗传学分析结果显示: 该种舌鳎属样品(Cynoglossus sp.)与短吻红舌鳎和长吻红舌鳎的遗传距离最为接近, 三者K2P遗传距离为0.006-0.009; 短吻三线舌鳎(C. abbreviatus)与紫斑舌鳎(C. purpureomaculatus)亲缘关系最近, 两者K2P遗传距离仅为0.002。以带纹条鳎(Zebrias zebra)为外类群构建的NJ系统发育树显示, 该种舌鳎属样品与短吻红舌鳎和长吻红舌鳎以及短吻三线舌鳎与紫斑舌鳎分别聚为一支, 其他种类各聚为一支。综合形态特征和COI基因比对结果, 确定该舌鳎属鱼卵、仔鱼样品为短吻红舌鳎。

关键词: 舌鳎属, 同种异名, 鱼类浮游生物, COI, 鉴别

Abstract

This study was designed to accurately identify Cynoglossus eggs and larvae (Cynoglossus sp.), and to accumulate base data for further study on the taxonomy and early identification of Cynoglossus fishes. Cynoglossus sp. samples were collected in Qingshan Bay, Qingdao, between May and October 2015. Three eggs, 3 larvae and a total of 5 individuals from 2 species (C. joyneri and C. lighti) in the Jiaozhou Bay area were barcoded using COI, sequenced, and compared with 6 Cynoglossus species found in the Shandong Peninsula and recorded in GenBank. Under a light microscope, the egg had a circular shape and floated within a wider perivitelline space; eggs ranged in diameter from 0.68 to 0.87 mm with a mean diameter of 0.73±0.03 mm; eggs had a single layer membrane and were smooth and transparent; the oil globules were located opposite the yolk sac, ranged from 6 to 15 in number, and diameter ranged from 0.04 to 0.10 mm (mean diameter: 0.07±0.01 mm); there were obvious characteristics including the 2 dorsal fin rays and pigmentation and body length changes in the period of metamorphosis for larvae identification. Results of genetic analysis showed that the average K2P distances pairwise-species in Cynoglossus sp., C. joyneri and C.lighti ranged from 0.006 to 0.009, and was 0.002 for C. abbreviatus and C. purpureomaculatus. The two groups were placed in two different clades with the support of a high bootstrap value and had a close genetic relationship, separately. The neighbor-joining (NJ) phylogenetic trees were constructed for COI gene sequences of 8 species using MEGA 6.0, and Zebrias zebra was included as an outgroup. The above analysis and results showed that the Cynoglossus sp. is C. joyneri.

Key words: Cynoglossus, synonym, ichthyoplankton, COI, identification

周晓梦, 郭书新, 宋娜, 张秀梅 (2017) 短吻红舌鳎鱼卵、仔鱼的DNA条形码和形态学鉴定. 生物多样性, 25, 847-855. DOI: 10.17520/biods.2017094.

Xiaomeng Zhou, Shuxin Guo, Na Song, Xiumei Zhang (2017) Identification of Cynoglossus joyneri eggs and larvae by DNA barcoding and morphological method. Biodiversity Science, 25, 847-855. DOI: 10.17520/biods.2017094.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.biodiversity-science.net/CN/10.17520/biods.2017094

https://www.biodiversity-science.net/CN/Y2017/V25/I8/847

图/表 7

参考文献 33

[1]	Ahlstrom EH, Moser HG (1976) Eggs and larvae of fishes and their role in systematic investigations and in fisheries. Revue des Travaux de l’Institut des Pêches Maritimes, 40, 379-398.
[2]	Ball SL, Hebert PDN, Burian SK, Webb JM (2005) Biological identifications of mayflies (Ephemeroptera) using DNA barcodes. Journal of the North American Benthological Society, 24, 508-524.
[3]	Bian XD, Zhang XM, Xiao YS, Gao TX, Wan RJ (2007) Identification of fish eggs of Japanese halfbeak (Hyporhamphus sajori) using mtDNA sequencing. Periodical of Ocean University of China, 37(S1), 111-116. (in Chinese with English abstract)
	[卞晓东, 张秀梅, 肖永双, 高天翔, 万瑞景 (2007) 线粒体DNA序列在沙氏下鱵鱼鱼卵鉴别上的应用. 中国海洋大学学报(自然科学版), 37(S1), 111-116.]
[4]	Carpenter KE, Niem VH (2001) FAO Species Identification Guide for Fishery Purposes: The Living Marine Resources of the Western Central Pacific, pp. 3890-3891. FAO, Rome.
[5]	Chen YX, Wu RX, Liang N, Liu J (2015) Phylogenetic relationship in family Sparidae of China in mitochondrial COI gene sequence. Oceanologia et Limnologia Sinica, 46, 611-619. (in Chinese with English abstract)
	[陈咏霞, 吴仁协, 梁娜, 刘静 (2015) 基于线粒体COI基因序列的中国鲷科鱼类系统进化关系. 海洋与湖沼, 46, 611-619.]
[6]	Gao DK (2015) Applying Basic Research on the Morphological and Molecular Approaches to Identifying the Ichthyoplankton. PhD dissertation, Ocean University of China, Qingdao. (in Chinese with English abstract)
	[高东奎 (2015) 鱼类浮游生物形态及分子鉴定方法的应用基础研究. 博士学位论文, 中国海洋大学, 青岛.]
[7]	General Administration of Quality Supervision, Inspection and Quarantine of China, Standardization Administration of China(2007) Specifications for Oceanographic Survey-- Part 6: Marine Biological Survey. China Standards Press, Beijing. (in Chinese)
	[中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会(2007) 海洋调查规范第6部分: 海洋生物调查. 中国标准出版社, 北京.]
[8]	Gu SO, Hwang HJ, Kim JK (2009) Morphological description of Cynoglossus joyneri larvae collected from the Yellow Sea. Korean Journal of Fisheries and Aquatic Sciences, 42, 725-728. (in Korean with English abstract)
[9]	Hebert PDN, Cywinska A, Ball SL (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London B: Biological Sciences, 270, 313-321.
[10]	Li SZ, Wang HM (1995) Fauna Sinica: Osteichthyes. Pleuronectiformes. Science Press, Beijing. (in Chinese)
	[李思忠, 王惠民 (1995) 中国动物志硬骨鱼纲鲽形目. 科学出版社, 北京.]
[11]	Li XM, Ye ZJ, Li ZG, Hu HS, Zou Q (2016) Spatial and temporal changes of the egg size changes of Japanese anchovy (Engraulis japonicus) inhabiting the near shore spawning ground of central Yellow Sea. Periodical of Ocean University of China, 46(2), 54-60. (in Chinese with English abstract)
	[李秀梅, 叶振江, 李增光, 胡海生, 邹全 (2016) 黄海中部近岸产卵场日本鳀卵子大小的时空变化. 中国海洋大学学报(自然科学版), 46(2), 54-60.]
[12]	Liu SF, Liu JX, Zhuang ZM, Gao TX, Han ZQ, Chen DG (2010a) Monophyletic origin and synonymic phenomena in the sub-family Cynoglossinae inferred from mitochondrial DNA sequences. Biodiversity Science, 18, 275-282. (in Chinese with English abstract)
	[柳淑芳, 刘进贤, 庄志猛, 高天翔, 韩志强, 陈大刚 (2010a) 舌鳎亚科鱼类单系起源和同种异名的线粒体DNA证据. 生物多样性, 18, 275-282.]
[13]	Liu SF, Chen LL, Dai FQ, Zhuang ZM (2010b) Applicaction of DNA barcoding gene CO1 for classifying family Sciaenidae. Oceanologia et Limnologia Sinica, 41, 223-232. (in Chinese with English abstract)
	[柳淑芳, 陈亮亮, 戴芳群, 庄志猛 (2010b) 基于线粒体CO1基因的DNA条形码在石首鱼科(Sciaenidae)鱼类系统分类中的应用. 海洋与湖沼, 41, 223-232.]
[14]	Menon AGK (1977) A Systematic Monograph of the Tongue Soles of the Genus Cynoglossus Hamilton-Buchanan (Pisces, Cynoglossidae). Smithsonian Institution Press, Washington, DC.
[15]	Miao XG, Jiang JX, Shi W, Wang ZM, Wang SY (2013) Phylogenetic relationship of Cynoglossinae based on COI barcoding marker. Journal of Tropical Oceanography, 32(5), 85-92. (in Chinese with English abstract)
	[苗宪广, 江金霞, 时伟, 王忠明, 王淑英 (2013) 舌鳎亚科Cynoglossinae鱼类基于COI条形码的系统发育关系. 热带海洋学报, 32(5), 85-92.]
[16]	Minami T (1983) The early life history of a tongue fish Cynoglossus joyneri. Bulletin of the Japanese Society of Scientific Fisheries, 49, 719-724. (in Japanese with English abstract)
[17]	Nakabo T (2000) Fishes of Japan with Pictorial Keys to the Species, 2nd ed. Tokai University Press, Tokyo. (in Japanese)
[18]	Ni HE, Zheng ZM (2002) Comparison of shapes and growth features of three species in Cynoglossus. Marine Sciences, 26(12), 61-64. (in Chinese with English abstract)
	[倪海儿, 郑忠明 (2002) 几种舌鳎的形态和生长特性的比较. 海洋科学, 26(12), 61-64.]
[19]	Pasco-Viel E, Charles C, Chevret P, Semon M, Tafforeau P, Viriot L, Laudet V (2010) Evolutionary trends of the pharyngeal dentition in Cypriniformes (Actinopterygii: Ostariophysi). PLoS ONE, 5, e11293.
[20]	Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning. Cold Spring Harbor Laboratory Press, New York.
[21]	Sharina SN, Kartavtsev YP (2010) Phylogenetic and taxonomic analysis of flatfish species (Teleostei, Pleuronectiformes) inferred from the primary nucleotide sequence of cytochrome oxidase 1 gene (Co-1). Russian Journal of Genetics, 46, 356-361.
[22]	Song C, Yu YN, Zhang T, Yang G, Zhang LZ (2014) Phylogenetic analysis of Cynoglossidae in the Yangtze Estuary based on partial sequence of mitochondrial COI. Chinese Journal of Zoology, 49, 716-726. (in Chinese with English abstract)
	[宋超, 于亚男, 张涛, 杨刚, 章龙珍 (2014) 基于线粒体COI基因部分序列的长江口舌鳎科鱼类系统分类研究. 动物学杂志, 49, 716-726.]
[23]	Su JX (1995) Fish and Marine Fish Culture, 2nd ed. China Agriculture Press, Beijing. (in Chinese)
	[苏锦祥 (1995) 鱼类学与海水鱼类养殖, 第二版. 中国农业出版社, 北京.]
[24]	Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques, 10, 506-513.
[25]	Wan RJ, Jiang YW (2000) The species and biological characteristics of the eggs and larvae of osteichthyes in the Bohai and Yellow Sea. Journal of Shanghai Fisheries University, 9, 290-297. (in Chinese with English abstract)
	[万瑞景, 姜言伟 (2000) 渤、黄海硬骨鱼类鱼卵与仔稚鱼种类组成及其生物学特征. 上海水产大学学报, 9, 290-297.]
[26]	Wan RJ, Jiang YW, Zhuang ZM (2004) Morphological and developmental characters at the early stages of the tonguefish Cynoglossus semilaevis. Acta Zoologica Sinica, 50, 91-102. (in Chinese with English abstract)
	[万瑞景, 姜言伟, 庄志猛 (2004) 半滑舌鳎早期形态及发育特征. 动物学报, 50, 91-102.]
[27]	Wang ZD, Guo YS, Chen RL, He XY, Liu CW, Liu Y (2009) COI barcoding sequences of teleosts in the South China Sea. Oceanologia et Limnologia Sinica, 40, 608-614. (in Chinese with English abstract)
	[王中铎, 郭昱嵩, 陈荣玲, 何晓莹, 刘楚吾, 刘筠 (2009) 南海常见硬骨鱼类COI条码序列. 海洋与湖沼, 40, 608-614.]
[28]	Ward RD, Zemlak TS, Innes BH, Last PR, Hebert PD (2005) DNA barcoding Australia’s fish species. Philosophical Transactions of the Royal Society of London B: Biological Sciences, 360, 1847-1857.
[29]	Xu H, Li J, Kong XY, Xiao ZZ, Li YL, Zhang YC, Shi W, Wei ZP, Yu ZN (2008) Phylogenetic relationship and length variation in the first ribosomal internal transcribed spacer of Cynoglossinae species. Oceanologia et Limnologia Sinica, 39, 35-41. (in Chinese with English abstract)
	[徐晖, 李军, 孔晓瑜, 肖志忠, 李玉龙, 张艳春, 时伟, 位正鹏, 喻子牛 (2008) 6种舌鳎亚科鱼类ITS1序列长度多态性及系统分析. 海洋与湖沼, 39, 35-41.]
[30]	Yagi Y, Kinoshita I, Fujita S, Ueda H, Aoyoma D (2009) Comparison of the early life histories of two Cynoglossus species in the inner estuary of Ariake Bay, Japan. Ichthyological Research, 56, 363-371.
[31]	Yang DL, Wu GZ, Pang HY (1983) The morphology of the early stage of longuefishes, Cynoglossus semilaevis Günther and C. joyneri Günther, in the Bohai Bay. Marine Sciences, 7(2), 29-32. (in Chinese with English abstract)
	[杨东莱, 吴光宗, 庞红艳 (1983) 渤海半滑舌鳎及焦氏舌鳎的鱼卵和仔稚鱼的形态. 海洋科学, 7(2), 29-32.]
[32]	Zhou CW, Cheng QT (1997) Ichthyography of Shandong. Shandong Science and Technology Press, Ji’nan. (in Chinese)
	[周才武, 成庆泰 (1997) 山东鱼类志. 山东科学技术出版社, 济南.]
[33]	Zhou MY, Chen X, Yang SY (2015) Identification of several fish eggs and larvae by DNA barcoding in Xiamen Water. Marine Environmental Science, 34(1), 120-125, 135. (in Chinese with English abstract)
	[周美玉, 陈骁, 杨圣云 (2015) 采用DNA条形码技术对厦门海域鱼卵、仔稚鱼种类的鉴定. 海洋环境科学, 34(1), 120-125, 135.]

形态特征 Morphological characters	短吻红舌鳎 C. joyneri			长吻红舌鳎 C. lighti
形态特征 Morphological characters	Li & Wang, 1995	Zhou & Cheng, 1997	Nakabo, 2000	Li & Wang, 1995	Zhou & Cheng, 1997	Nakabo, 2000
上、中侧线鳞间鳞数 Scales between upper and middle lateral line	12-13	11-12	/	10-11	10-11	/
体长/体高 Body length/body depth	3.6-4.4	3.8-4.0	3.4-4.1	3.8-4.5	4.2-5.0	3.8-4.5
头长/头高 Head length/head depth	≤1	/	/	>1	/	/

形态特征 Morphological characters	短吻红舌鳎 C. joyneri			长吻红舌鳎 C. lighti
形态特征 Morphological characters	Li & Wang, 1995	Zhou & Cheng, 1997	Nakabo, 2000	Li & Wang, 1995	Zhou & Cheng, 1997	Nakabo, 2000
上、中侧线鳞间鳞数 Scales between upper and middle lateral line	12-13	11-12	/	10-11	10-11	/
体长/体高 Body length/body depth	3.6-4.4	3.8-4.0	3.4-4.1	3.8-4.5	4.2-5.0	3.8-4.5
头长/头高 Head length/head depth	≤1	/	/	>1	/	/

编号Number	种类 Species	序列编号Sequence numbers	序列来源 Sources of sequences
1	舌鳎属样品 Cynoglossus sp.	egg.1-egg.3; lar.1-lar.3	本研究 This study
2	短吻红舌鳎 C. joyneri	joy.1-joy.5	本研究(1-3); JQ738430.1; KF979127.1
3	长吻红舌鳎 C. lighti	lig.1-lig4	本研究(1-2); DQ116752.1; HQ711865.1
4	宽体舌鳎 C. robustus	rob.1-rob.3	KT718591.1; HM180552.1; HM180553.1
5	半滑舌鳎 C. semilaevis	sem.1-sem.3	JN242742.1; JN242743.1; JN242744.1
6	紫斑舌鳎 C. purpureomaculatus	pur.1-pur.3	DQ116749.1; JQ738570.1; JQ738571.1
7	短吻三线舌鳎 C. abbreviatus	abb.1	DQ116750.1
8	带纹条鳎 Zebrias zebra	zeb.1-zeb.3	JQ738566.1; JQ738567.1; JQ738568.1

编号Number	种类 Species	序列编号Sequence numbers	序列来源 Sources of sequences
1	舌鳎属样品 Cynoglossus sp.	egg.1-egg.3; lar.1-lar.3	本研究 This study
2	短吻红舌鳎 C. joyneri	joy.1-joy.5	本研究(1-3); JQ738430.1; KF979127.1
3	长吻红舌鳎 C. lighti	lig.1-lig4	本研究(1-2); DQ116752.1; HQ711865.1
4	宽体舌鳎 C. robustus	rob.1-rob.3	KT718591.1; HM180552.1; HM180553.1
5	半滑舌鳎 C. semilaevis	sem.1-sem.3	JN242742.1; JN242743.1; JN242744.1
6	紫斑舌鳎 C. purpureomaculatus	pur.1-pur.3	DQ116749.1; JQ738570.1; JQ738571.1
7	短吻三线舌鳎 C. abbreviatus	abb.1	DQ116750.1
8	带纹条鳎 Zebrias zebra	zeb.1-zeb.3	JQ738566.1; JQ738567.1; JQ738568.1

碱基 Base	总频率 Total	密码子第1位点 The first position of codon	密码子第2位点 The second position of codon	密码子第3位点 The third position of codon
T	31.3	18.0	43.8	32.1
C	26.5	24.5	28.1	27.1
A	25.6	27.4	14.5	34.8
G	16.6	30.1	13.6	6.0
A+T	56.9	45.4	58.3	66.9
G+C	43.1	54.6	41.7	33.1

短吻红舌鳎鱼卵、仔鱼的DNA条形码和形态学鉴定

Identification of Cynoglossus joyneri eggs and larvae by DNA barcoding and morphological method

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 33

相关文章 11

编辑推荐

Metrics

本文评价

种类 Species	样本量 Sample size	平均GC含量 Average GC content (%)
种类 Species	样本量 Sample size	全部位点 Total positions	第1位点 The first position	第2位点 The second position	第3位点 The third position
1舌鳎属样品 Cynoglossus sp.	6	42.9	57.2	42	29.5
2短吻红舌鳎 C. joyneri	5	43	56.9	42	29.9
3长吻红舌鳎 C. lighti	4	43	57.3	42	29.9
4宽体舌鳎 C. robustus	3	42.5	53.3	42	31.9
5半滑舌鳎 C. semilaevis	3	40.8	52.4	42	28.2
6紫斑舌鳎 C. purpureomaculatus	3	43.1	52.3	42	34.9
7短吻三线舌鳎 C. abbreviatus	1	42.9	51.8	42	34.9
8带纹条鳎 Zebrias zebra	3	48.4	53.9	42.5	49.0

种间 Pairwise-species	1	2	3	4	5	6	7	8
1舌鳎属样品 Cynoglossus sp.	0.009
2短吻红舌鳎 C. joyneri	0.009	0.008
3长吻红舌鳎 C. lighti	0.008	0.006	0.004
4宽体舌鳎 C. robustus	0.236	0.240	0.240	0.002
5半滑舌鳎 C. semilaevis	0.189	0.192	0.190	0.208	0.000
6紫斑舌鳎 C. purpureomaculatus	0.181	0.182	0.181	0.200	0.113	0.000
7短吻三线舌鳎C. abbreviatus	0.182	0.185	0.184	0.203	0.116	0.002	n/c
8带纹条鳎 Zebrias zebra	0.245	0.245	0.244	0.277	0.278	0.302	0.304	0.002

[1]	翁茁先,黄佳琼,张仕豪,余锴纯,钟福生,黄勋和,张彬. 利用线粒体COI基因揭示中国乌骨鸡遗传多样性和群体遗传结构[J]. 生物多样性, 2019, 27(6): 667-676.
[2]	刘青青, 董志军. 基于线粒体COI基因分析钩手水母的群体遗传结构[J]. 生物多样性, 2018, 26(11): 1204-1211.
[3]	杨倩倩, 刘苏汶, 茹炜岽, 刘光富, 俞晓平. 基于DNA条形码技术对浙江省外来入侵福寿螺进行分子鉴定[J]. 生物多样性, 2016, 24(3): 341-350.
[4]	李海涛, 张保学, 高阳, 时小军, 周鹏. DNA条形码技术在海洋贝类鉴定中的实践: 以大亚湾生态监控区为例[J]. 生物多样性, 2015, 23(3): 299-305.
[5]	王太, 张艳萍, 管丽红, 杜岩岩, 娄忠玉, 焦文龙. 甘肃省鱼类资源现状及DNA条形码在鱼类物种鉴定中的应用[J]. 生物多样性, 2015, 23(3): 306-313.
[6]	何锴, 王文智, 李权, 罗培鹏, 孙悦华, 蒋学龙. DNA条形码技术在小型兽类鉴定中的探索: 以甘肃莲花山为例[J]. 生物多样性, 2013, 21(2): 197-205.
[7]	陈哲, 张姜, 傅杭飞, 许争争, 邓坤正, 张加勇. 基于形态特征和线粒体COI基因探讨扶桑绵粉蚧物种的有效性并记述一体色变异型扶桑绵粉蚧[J]. 生物多样性, 2012, 20(4): 443-450.
[8]	马英, 李海龙, 鲁亮, 刘起勇. DNA条形码技术在青海海东地区小型兽类鉴定中的应用[J]. 生物多样性, 2012, 20(2): 193-198.
[9]	隆沂峄, 杨丽媛, 廖万金. 利用PCR-RFLP技术鉴定传粉榕小蜂隐种混合样品的物种组成[J]. 生物多样性, 2010, 18(4): 414-419.
[10]	刘淑德, 线薇微. 长江口及其邻近水域鱼类浮游生物群落的时空格局[J]. 生物多样性, 2009, 17(2): 151-159.
[11]	阎雪岚, 唐文乔, 杨金权. 基于线粒体控制区的序列变异分析中国东南部沿海凤鲚种群遗传结构[J]. 生物多样性, 2009, 17(2): 143-150.