寄主植物转换对B型烟粉虱和温室粉虱淀粉酶及蛋白酶活性的影响

doi:10.3724/SP.J.1003.2008.08050

生物多样性 ›› 2008, Vol. 16 ›› Issue (4): 313-320. DOI: 10.3724/SP.J.1003.2008.08050

所属专题：昆虫多样性与生态功能

• 研究报告 • 下一篇

寄主植物转换对B型烟粉虱和温室粉虱淀粉酶及蛋白酶活性的影响

张桂芬¹, 雷芳¹^,², 万方浩¹^,^*(), 马骏¹^,², 杨玉国³

1 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100094
2 湖南农业大学生物安全科技学院, 长沙 410128
3 北京市顺义区马坡镇农业公司, 北京 101300

收稿日期:2008-03-05 接受日期:2008-07-02 出版日期:2008-07-20 发布日期:2008-07-20
通讯作者: 万方浩
作者简介:第一联系人：** 同等贡献作者 The two authors contributed equally to this work
基金资助:
国家重点基础研究发展规划项目(2002CB111400)

Effects of plant species switching on dynamics of amylase and proteinase activity of Bemisia tabaci biotype B and Trialeurodes vaporariorum

Guifen Zhang¹, Fang Lei¹^,², Fanghao Wan¹^,^*(), Jun Ma¹^,², Yuguo Yang³

1 State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094
2 College of Bio-Safety Science and Technology, Hunan Agricultural University, Changsha 410128
3 Agricultural Corporations of Mapo Township, Shunyi, Beijing 101300

Received:2008-03-05 Accepted:2008-07-02 Online:2008-07-20 Published:2008-07-20
Contact: Fanghao Wan

摘要/Abstract

摘要：

B型烟粉虱(Bemisia tabaci)和温室粉虱(Trialeurodes vaporariorum)在华北地区常混合发生, 为了揭示寄主植物在两种粉虱生态位竞争中的作用, 以番茄饲养的B型烟粉虱与温室粉虱为实验种群, 研究了转换取食棉花、甘蓝及玉米后, 其体内淀粉酶及蛋白酶的变化。结果表明, 无论在饲养寄主上还是转换植物上, B型烟粉虱淀粉酶活性较温室粉虱高1.49-1.66倍, 改变植物种类对两种粉虱淀粉酶活性的影响不大。植物种类的改变对B型烟粉虱的蛋白酶活性没有显著影响, 而温室粉虱却受到明显抑制, 由番茄转换到甘蓝、棉花及玉米上, 温室粉虱蛋白酶活性下降了29.9-42.7%; 转换到甘蓝或棉花上, B型烟粉虱蛋白酶活性分别较温室粉虱高1.30倍和1.21倍, 而在玉米上两种粉虱间没有明显差异。B型烟粉虱在嗜食寄主甘蓝或非寄主玉米上取食, 其淀粉酶活性的动态变化趋势为激活—抑制型或抑制—激活型, 而温室粉虱激活—抑制不明显; 在B型烟粉虱嗜食、温室粉虱亦可利用的棉花上, 两种粉虱淀粉酶活性的动态趋势相仿。转换的植物种类不同, 两种粉虱蛋白酶活性的变化趋势各异; 在B型烟粉虱嗜食的甘蓝和棉花上, 其蛋白酶活性的动态趋势为激活—抑制型; 在非寄主玉米上虽亦为激活—抑制型, 但激活需时长且较为平缓; 而温室粉虱无论转换为寄主还是非寄主植物, 其蛋白酶活性的变化趋势均相仿且较为平缓。以上结果表明B型烟粉虱应对植物种类转换的能力较温室粉虱强。

关键词: B型烟粉虱, 温室粉虱, 淀粉酶, 蛋白酶, 动态变化趋势, 植物种类转换, 适应

Abstract

Bemisia tabaci biotype B (Bt) and Trialeurodes vaporariorum(Tv) are two whiteflies that often coexist on greenhouse-grown vegetable crops in northern China. The host plant species ofB. tabaci biotype B have been increasing with the spread of its invaded areas. To clarify the effects of plant species on the performances of the two species, amylase and proteinase activity dynamics of both whitefly species were evaluated when their host plant species switched from tomato (preferred by both species, also used as the control) to cotton (preferred by Bt and suitable for Tv), cabbage (preferred by Bt, but unsuitable for Tv) or maize (unsuitable for both species) plants. There were no prominent effects of plant species switching on the amylase activity of the two whitefly species. The amylase activity of Bt was 1.49-1.66 folds higher than that of Tv when fed on tomato or when switched to different host species. There were no effects on the proteinase activity of Bt when switched to a different host species, but that of Tv decreased by 29.9-42.7%. The proteinase activity of Bt was 1.30 and 1.21 folds higher than that of Tv when switched to cabbage and cotton plants, respectively. There were no interspecific differences in proteinase activity when switched to maize plants. Bt amylase activity dynamics were expressed as activation then inhibition or inhibition then activation when switched to cabbage and maize plants; however, those of Tv were smooth. Amylase activity dynamics were similar for both species when switched to cotton. Although proteinase activity dynamics of Bt were expressed as activation then inhibition when switched to any of the three plant species tested, the activation period was longer when switched to maize plants. In the case of Tv, proteinase activity dynamics were similarly smooth regardless of which plant species switched to. Our results indicated that Bt was more adaptable to plant species switching than T. vaporariorum.

Key words: Bemisia tabaci biotype B, Trialeurodes vaporariorum, amylase, proteinase, dynamics, plant species switching, adaptability

张桂芬, 雷芳, 万方浩, 马骏, 杨玉国 (2008) 寄主植物转换对B型烟粉虱和温室粉虱淀粉酶及蛋白酶活性的影响. 生物多样性, 16, 313-320. DOI: 10.3724/SP.J.1003.2008.08050.

Guifen Zhang, Fang Lei, Fanghao Wan, Jun Ma, Yuguo Yang (2008) Effects of plant species switching on dynamics of amylase and proteinase activity of Bemisia tabaci biotype B and Trialeurodes vaporariorum. Biodiversity Science, 16, 313-320. DOI: 10.3724/SP.J.1003.2008.08050.

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链接本文: https://www.biodiversity-science.net/CN/10.3724/SP.J.1003.2008.08050

https://www.biodiversity-science.net/CN/Y2008/V16/I4/313

图/表 4

表1 寄主植物种类转换对B型烟粉虱和温室粉虱淀粉酶活性的影响

Table 1 Effects of plant species switching on amylase activity of Bemisia tabacibiotype B and Trialeurodes vaporariorum (mean±SE, μg•mg-1•min-1)

寄主植物种类 Host plant species	B型烟粉虱 Bemisia tabaci biotype B		温室粉虱 Trialeurodes vaporariorum
寄主植物种类 Host plant species	淀粉酶活性 Amylase activity	相对比值* Ratio		淀粉酶活性 Amylase activity	相对比值* Ratio
甘蓝 Cabbage	61.89±5.59^{a A}	0.89	38.94±4.40^{a B}		0.93
棉花 Cotton	61.00±5.04^{a A}	0.88	40.98±3.07^{a B}		0.98
玉米 Maize	70.99±8.18^{a A}	1.02	42.86±3.50^{a B}		1.02
番茄(对照) Tomato (CK)	69.49±7.54^{a A}	1.00	41.89±3.86^{a B}		1.00

表2 寄主植物种类转换对B型烟粉虱和温室粉虱蛋白酶活性的影响

Table 2 Effects of plant species switching on proteinase activity of Bemisia tabacibiotype B and Trialeurodes vaporariorum (mean±SE, µg•mg-1•min-1)

寄主植物种类 Host plant species	B型烟粉虱 Bemisia tabaci biotype B		温室粉虱 Trialeurodes vaporariorum
寄主植物种类 Host plant species	蛋白酶活性 Proteinase activity	相对比值* Ratio	蛋白酶活性 Proteinase activity	相对比值* Ratio
甘蓝 Cabbage	28.29±3.53^{a A}	0.96	21.68±2.43^{abc B}	0.70
棉花 Cotton	21.52±2.75^{a A}	0.73	17.72±2.02^{a A}	0.57
玉米 Maize	20.57±4.00^{a A}	0.70	19.17±3.44^{ab A}	0.62
番茄(对照) Tomato (CK)	29.36±3.90^{a A}	1.00	30.93±3.87^{c A}	1.00

图1 寄主植物种类转换对两种粉虱淀粉酶活性变化趋势的影响。(A: 甘蓝, B: 棉花, C: 玉米; * 图中数据为以两种粉虱在番茄上的淀粉酶活性为对照的相对比值; 同一检测时间字母相同表示两种粉虱在同一种植物上淀粉酶活性在P = 0.05水平差异不显著(成对数据t检验))。

Fig. 1 Effects of plant species switching on dynamics of amylase activity of Bemisia tabacibiotype B and Trialeurodes vaporariorum. (A), Cabbage; (B), Cotton; (C), Maize. * The data are ratios of amylase activity on tomato to other host plant species at the same testing time. Data at the same testing time followed by the same letter are not significantly different at P=0.05 between B. tabacibiotype B and T. vaporariorum when feeding on the same plant species (Paired samples ttest).

图2 寄主植物种类转换对两种粉虱蛋白酶活性变化趋势的影响。(A: 甘蓝, B: 棉花, C: 玉米; * 图中数据为以两种粉虱在番茄上的蛋白酶活性为对照的相对比值; 同一检测时间字母相同表示两种粉虱在同一种植物上的蛋白酶活性在P = 0.05水平差异不显著(成对数据t检验))。

Fig. 2 Effects of plant species switching on dynamics of proteinase activity of Bemisia tabacibiotype B and Trialeurodes vaporariorum. (A), Cabbage; (B), Cotton; (C), Maize. * The data are ratios of proteinase activity on tomato to other host plant species at the same testing time. Data at the same testing time followed by the same letter are not significantly different at P=0.05 between B. tabaci biotype B and T. vaporariorum when feeding on the same plant species (Paired samples ttest).

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寄主植物转换对B型烟粉虱和温室粉虱淀粉酶及蛋白酶活性的影响

Effects of plant species switching on dynamics of amylase and proteinase activity of Bemisia tabaci biotype B and Trialeurodes vaporariorum

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