Biodiversity Science ›› 2017, Vol. 25 ›› Issue (4): 409-417.doi: 10.17520/biods.2016331

• Original Papers: Animal Diversity • Previous Article     Next Article

Diversity investigation and application of DNA barcoding of Acridoidea from Baiyangdian Wetland

Jinfeng Hao, Xiaohong Zhang, Yusong Wang, Jinlin Liu, Yongchao Zhi, Xinjiang Li*()   

  1. The Key Laboratory of Zoological Systematics and Application, College of Life Sciences, Hebei University, Baoding, Hebei 071002
  • Received:2016-11-22 Accepted:2017-04-10 Online:2017-04-20
  • Li Xinjiang E-mail:hbulxj@163.com

Both the species diversity and distribution pattern of the superfamily Acridoidea of the suborder Caelifera have important contributions to understanding the local biodiversity of the Baiyangdian Wetland. This research tries to study the species diversity and distribution pattern of the superfamily Acridoidea within the Baiyangdian Wetland and test the feasibility of DNA barcoding in species identification for this superfamily. Sequences of the cox1 gene were obtained from 97 individuals of 21 species of the superfamily Acridoidea. Phylogenetic, genetic distance and sequence difference threshold analyses using the Neighbor Joining (NJ), Automatic Barcode Gap Discovery (ABGD) and Molecular Defined Operational Taxonomic Units (MOTU) methods, respectively, were performed for these and the 25 additional sequences of 10 species downloaded from GenBank. The results indicate that there are 34 species, 23 genera, and 6 families of the superfamily Acridoidea insects around the farmland, dam, and grassland of the Baiyangdian Wetland, including a new-record genus, Euchorthippus, and three new-record species, Euchorthippus unicolor, Atractomorpha psittacina and Oxya japonica. The DNA barcoding technology therefore is very efficient and helpful for identifying the species of the superfamily Acridoidea, although the morphological approach is still playing a key role in the species identifications.

Key words: Orthoptera, Acridoidea, Baiyangdian Wetland, diversity, DNA barcoding

Fig. 1

The collecting localities of grasshoppers from Baiyangdian Wetland"

Table 1

Species checklist of Acridoidea from Baiyangdian Wetland with the specimen information"

科名
Family
属名
Genus
种名
Species
采集地点
Collection location
采集时间
Collection time
癞蝗科 Pamphagidae 笨蝗属 Haplotropis 笨蝗 Haplotropis brunneriana
锥头蝗科
Pyrgomorphidae
负蝗属 Atractomorpha 短额负蝗 Atractomorpha sinensis 寨里 Zhaili
崔公堤 Cuigongdi
大王镇 Dawangzhen
大树刘庄 Dashuliuzhuang
2011.8
2014.9
2015.8
2015.9
柳枝负蝗 Atractomorpha psittacina 宋庄 Songzhuang
杨庄子 Yangzhuangzi
赵北口 Zhaobeikou
2015.8
2011.8
2014.9
令箭负蝗 Atractomorpha sagittaris 南曲堤 Nanqudi
大树刘庄 Dashuliuzhuang
端村 Duancun
2011.8
2015.9
2014.9
长额负蝗 Atractomorpha lata 圈头 Quantou
崔公堤 Cuigongdi
杨庄子 Yangzhuangzi
宋庄 Songzhuang
2011.8
2014.9
2011.8
2015.8
斑腿蝗科 Catantopidae 稻蝗属 Oxya 日本稻蝗 Oxya japonica 寨里 Zhaili
大王镇 Dawangzhen
端村 Duancun
2011.8
2015.8
2014.9
中华稻蝗 Oxya chinensis 圈头 Quantou
南曲堤 Nanqudi
大树刘庄 Dashuliuzhuang
2011.8
2011.8
2015.9
蔗蝗属 Hieroglyphus 斑角蔗蝗 Hieroglyphus annulicornis
棉蝗属 Chondracris 棉蝗 Chondracris rosea rosea
黄脊蝗属 Patanga 日本黄脊蝗 Patanga japonica
星翅蝗属 Calliptamus 短星翅蝗 Calliptamus abbreviatus 杨庄子 Yangzhuangzi
赵北口 Zhaobeikou
大王镇 Dawangzhen
2011.8
2014.9
2015.8
素木蝗属 Shirakiacris 长翅素木蝗 Shirakiacris shirakii 南曲堤 Nanqudi
大树刘庄 Dashuliuzhuang
赵北口 Zhaobeikou
2011.8
2015.9
2014.9
斑翅蝗科 Oedipodidae 飞蝗属 Locusta 东亚飞蝗
Locusta migratoria manilensis
崔公堤 Cuigongdi
大王镇 Dawangzhen
赵北口 Zhaobeikou
宋庄 Songzhuang
2014.9
2015.8
2014.9
2015.8
车蝗属 Gastrimargus 云斑车蝗 Gastrimargus marmoratus
尖翅蝗属 Epacromius 大垫尖翅蝗 Epacromius coerulipes 端村 Duancun
赵北口 Zhaobeikou
崔公堤 Cuigongdi
2014.9
2011.8
2014.9
小车蝗属 Oedaleus 黄胫小车蝗
Oedaleus infernalis infernalis
大王镇 Dawangzhen
端村 Duancun
南曲堤 Nanqudi
崔公堤 Cuigongdi
2015.8
2014.9
2011.8
2014.9
亚洲小车蝗 Oedaleus asiaticus 杨庄子 Yangzhuangzi
南曲堤 Nanqudi
崔公堤 Cuigongdi
赵北口 Zhaobeikou
端村 Duancun
2011.8
2011.8
2014.9
2014.9
2014.9
赤翅蝗属 Celes 大赤翅蝗 Celes skalozubovi akitanus
疣蝗属 Trilophidia 疣蝗 Trilophidia annulata 崔公堤 Cuigongdi
赵北口 Zhaobeikou
杨庄子 Yangzhuangzi
2014.9
2014.9
2011.8
绿纹蝗属 Aiolopus 花胫绿纹蝗 Aiolopus tamulus 崔公堤 Cuigongdi
大王镇 Dawangzhen
赵北口 Zhaobeikou
杨庄子 Yangzhuangzi
2014.9
2015.8
2014.9
2011.8
异痂蝗属 Bryodemella 轮纹异痂蝗 Bryodemella tuberculatum dilutum
网翅蝗科
Arcypteridae
曲背蝗属 Pararcyptera 宽翅曲背蝗
Pararcyptera microptera meridionalis
网翅蝗属 Arcyptera 隆额网翅蝗 Arcyptera coreana
雏蝗属 Chorthippus 黑翅雏蝗 Chorthippus aethalinus
小翅雏蝗 Chorthippus fallax
北方雏蝗 Chorthippus hammarstroemi
狭翅雏蝗 Chorthippus dubius
白纹雏蝗 Chorthippus albonemus
华北雏蝗
Chorthippus brunneus huabeiensis
东方雏蝗 Chorthippus intermedius
异爪蝗属
Euchorthippus
素色异爪蝗 Euchorthippus unicolor 寨里 Zhaili
崔公堤 Cuigongdi
大王镇 Dawangzhen
2011.8
2014.9
2015.8
剑角蝗科
Acrididae
鸣蝗属
Mongolotettix
条纹鸣蝗
Mongolotettix japonicus vittatus
剑角蝗属 Acrida 中华剑角蝗 Acrida cinerea 南曲堤 Nanqudi
大树刘庄 Dashuliuzhuang
大王镇 Dawangzhen
崔公堤 Cuigongdi
寨里 Zhaili
2011.8
2015.9
2015.8
2014.9
2011.8
戛蝗属 Gonista 二色戛蝗 Gonista bicolor 崔公堤 Cuigongdi
大王镇 Dawangzhen
寨里 Zhaili
2014.9
2015.8
2011.8

Fig. 2

NJ Phylogenetic tree, ABGD result and MOTU result of Acridoidea from Baiyangdian Wetland based on cox1 gene sequences"

Fig. 3

Automatic partition results of ABGD in 122 samples based on prior intraspecific divergence range of 0.001-0.1"

Fig. 4

Variation in the number of MOTUs defined at 0 to100 cut-off values for DNA barcodings"

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