Biodiversity Science ›› 2015, Vol. 23 ›› Issue (5): 649-657.doi: 10.17520/biods.2015032

Special Issue: Forest Dynamics Monitoring

• Orginal Article • Previous Article     Next Article

Soil bacterial diversity in the Baotianman deciduous broad-leaved forest

Aihua Zhao1, 2, Xiaojun Du1, *(), Jing Zang1, Shouren Zhang1, Zhihua Jiao3   

  1. 1 State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093
    2 University of Chinese Academy of Sciences, Beijing 100049
    3 School of Chemical and Environmental Engineering, China University of Mining & Technology (Beijing), Beijing 100083;
  • Received:2015-02-10 Accepted:2015-02-10 Online:2015-10-12
  • Du Xiaojun

Soil microbes play an essential role in forest ecosystems. The development of the high-throughput sequencing method provides an invaluable opportunity to further understand soil microbial communities. In this study, we investigated soil bacterial diversity in the Baotianman forest using the Illumina Miseq platform. Results showed that in the 31 soil sampling points, the number of bacterial taxa detected at different classification levels increased with increasing number of sampling points. When all 31 samples were considered, the number of relative bacterial groups included 45 phyla, 163 classes, 319 orders, 495 families, 785 genera and 42,632 OTUs. The mean values of relative bacterial taxa in the 31 samples were 34.2 phyla, 114.7 classes, 215.2 orders, 323.7 families, 446.6 genera, and 5,924.7 OTUs. At the classification level of phylum, class and order, the dominant groups were Proteobacteria (38.30%), α-Proteobacteria (18.08%), and Rhizobiales (10.62%), respectively. These preliminary findings suggest that Baotianman forest soils have high levels of bacterial diversity to some degree and provide basic information and knowledge to further understand the relationship between soil bacterial diversity and plant diversity and other related scientific questions.

Key words: forest, soil microbe, bacterial diversity, high-throughput sequencing, Proteobacteria, α-Proteobacteria, Rhizobiales, Baotianman National Nature Reserve

Fig. 1

Location of the 31 soil samples in the 25 ha Baotianman Forest Dynamics Plot"

Table 1

Sequence numbers and OTU numbers of individual samples"

Sample identity
Original sequence number
Original OTU number
Even sequence number
Even OTU number
0101 37,569 7,148 36,000 6,979
0105 39,949 5,671 36,000 5,320
0203 39,698 5,661 36,000 5,327
0218 39,337 5,579 36,000 5,320
0306 39,281 6,396 36,000 6,097
0318 39,760 6,263 36,000 5,906
0411 39,756 6,008 36,000 5,672
0606 39,636 6,642 36,000 6,286
0701 39,945 6,059 36,000 5,694
0819 39,864 6,324 36,000 5,933
0913 39,827 5,275 36,000 4,971
0919 39,720 5,958 36,000 5,645
1002 39,844 5,486 36,000 5,175
1006 39,551 6,658 36,000 6,322
1018 39,711 5,526 36,000 5,184
1104 39,746 6,380 36,000 6,036
1210 39,569 6,255 36,000 5,933
1212 39,659 6,016 36,000 5,691
1318 39,809 6,706 36,000 6,326
1405 39,825 5,910 36,000 5,547
1510 39,379 5,650 36,000 5,378
1512 37,588 5,555 36,000 5,413
1607 39,739 6,338 36,000 5,981
1615 39,212 6,246 36,000 5,978
1618 39,578 6,552 36,000 6,200
1704 39,495 6,405 36,000 6,024
1718 39,130 6,910 36,000 6,564
1808 39,739 6,833 36,000 6,498
1814 39,866 6,850 36,000 6,501
1902 39,155 7,283 36,000 6,920
1913 38,882 7,145 36,000 6,844

Fig. 2

The quantity of bacteria at different taxonomic levels in the 31 soil samples in the Baotianman Forest Dynamics Plot"

Fig. 3

Rarefaction curves of the observed bacterial quantity at different taxonomic levels varying with number of samples in the Baotianman Forest Dynamics Plot"

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