Biodiv Sci ›› 2012, Vol. 20 ›› Issue (6): 710-715.DOI: 10.3724/SP.J.1003.2012.06090

• Research Report • Previous Articles     Next Articles

Effect of zinc on DNA methylation diversity at different developmental stages of filial generation in Drosophila

Xinxin Guo, Xue Mao, Min Zhang*()   

  1. College of Life Sciences, Shaanxi Normal University, Xi’an 710062
  • Received:2012-04-01 Accepted:2012-06-16 Online:2012-11-20 Published:2013-01-04
  • Contact: Min Zhang

Abstract:

To examine the features of DNA methylation diversity, we used a zinc treated Canton-S strain of Drosophila melanogaster as a model organism in this experiment. Our results showed significant differences in DNA methylation polymorphisms among embryos, third instar larvae, pupae, and female and male individuals. After zinc treatment (at a concentration of 2.5%, 5%, and 10%) to the parental generation, the percentage of DNA methylation polymorphic bands of offspring (5-8 h embryo) in the experimental groups F1-1, F1-2, F1-3 were significantly higher than the control group. The experimental group F1-2 (zinc concentration 2.5%) in 5-8 h embryo was affected most by zinc treatment of the parental generation, and DNA methylation polymorphic band ratios in 12-15 h embryo, 18-21 h embryo and female D. melanogaster were significantly higher than the control groups. Our results indicate that the embryo stage contains the highest DNA methylation diversity, and appropriate concentration of zinc material can increase DNA methylation diversity of D. melanogaster offspring.

Key words: Drosophila, zinc, AFLP, DNA methylation