Genetic patterns of an invasive Procambarus clarkii population in the three river basins of Anhui Province

doi:10.3724/SP.J.1003.2010.398

Abstract

Abstract:

Procambarus clarkii is an alien invasive crayfish species that occurs in the major river basins along the middle and lower reaches of the Yangtze River. To understand genetic diversity and population structure of the species in the region, we collected 182 individuals from nine sites in four regions in the Yangtze, Huaihe, and Xin'anjiang River basins in Anhui Province. Using nine pairs of microsatellite markers, we studied the genetic differentiation, genetic structure and genetic clustering pattern of these P. clarkiipopulations. The expected heterozygosity (H_E) was 0.78 while the observed heterozygosity (H_O) was 0.36. Of the nine populations, the highest expected heterozygosity (0.76) was in the Wangjiang population, and the lowest (0.56) was for the Feixi population. Using a Bottleneck Test, we found a significant signal of heterozygote deficiency, indicating a recent decline in these populations. Our studies also showed that there were high levels of gene flow among populations, and a significant correlation found between genetic distance and geographic distance (r= 0.33, P< 0.05). We detected evidence of a significant jump dispersal event between the Xin’anjiang and Chengdonghu populations, thus indicating that human-mediated dispersal has played an important role in the present genetic structure ofProcambarus clarkiipopulation.

Key words: Procambarus clarkii, microsatellite DNA, genetic structure, biological invasion, species dispersal

Lingliang Cao, Lizhi Zhou, Baowei Zhang. Genetic patterns of an invasive Procambarus clarkii population in the three river basins of Anhui Province[J]. Biodiv Sci, 2010, 18(4): 398-407.

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URL: https://www.biodiversity-science.net/EN/10.3724/SP.J.1003.2010.398

https://www.biodiversity-science.net/EN/Y2010/V18/I4/398

Figures/Tables 8

References 64

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地方种群 Local populations	编号 Code	采集点 Sampling site	样本量 Sample size	期望杂合度 H_E	观测杂合度 H_O	平均等位基因数 MNA	私有等位基因数 Np
淮河 Huaihe River
城东湖 Chengdong Lake	Cd	霍邱黄泊渡 Huangbodu, Huoqiu	24	0.72	0.31	6.62	5
江淮之间 Between Yangtze River and Huaihe River
巢湖 Chaohu Lake	Ch	义城 Yicheng	7	0.65	0.24	4.89	5
巢湖 Chaohu Lake	Ch	长临河口 Changlin River Mouths	3	0.65	0.24	4.89	0
肥西 Feixi	Fx	柿树岗 Stream in Shishugang	12	0.56	0.39	4.44	3
合肥 Hefei	Hf	董铺水库 Dongpu Reservoir	6	0.57	0.33	4.75	2
合肥 Hefei	Hf	三十头支流 Stream in Sanshitou	9	0.57	0.33	4.75	1
长江 Yangtze River
菜子湖 Caizi Lake	Czh	王湾咀 Wangwanzui	7	0.67	0.47	9.89	4
		杨湾 Yangwan	3				0
		肖店 Xiaodian	21				6
		双店 Shuangdian	12				3
升金湖 Shengjin Lake	Sj	升金湖 Shenjin Lake	13	0.71	0.56	6.33	5
东至 Dongzhi	Dz	香口乡 Stream in Xiangkou	21	0.74	0.33	7.55	9
望江 Wangjiang	Wj	华阳河 Huayang River	20	0.76	0.17	7.44	18
新安江 Xin'anjiang River
新安江 Xin'anjiang River	Xa	屯光镇 Tunguang	24	0.64	0.39	6.00	3
总计 Total			182	0.67	0.35	-	-

地方种群 Local populations	编号 Code	采集点 Sampling site	样本量 Sample size	期望杂合度 H_E	观测杂合度 H_O	平均等位基因数 MNA	私有等位基因数 Np
淮河 Huaihe River
城东湖 Chengdong Lake	Cd	霍邱黄泊渡 Huangbodu, Huoqiu	24	0.72	0.31	6.62	5
江淮之间 Between Yangtze River and Huaihe River
巢湖 Chaohu Lake	Ch	义城 Yicheng	7	0.65	0.24	4.89	5
巢湖 Chaohu Lake	Ch	长临河口 Changlin River Mouths	3	0.65	0.24	4.89	0
肥西 Feixi	Fx	柿树岗 Stream in Shishugang	12	0.56	0.39	4.44	3
合肥 Hefei	Hf	董铺水库 Dongpu Reservoir	6	0.57	0.33	4.75	2
合肥 Hefei	Hf	三十头支流 Stream in Sanshitou	9	0.57	0.33	4.75	1
长江 Yangtze River
菜子湖 Caizi Lake	Czh	王湾咀 Wangwanzui	7	0.67	0.47	9.89	4
		杨湾 Yangwan	3				0
		肖店 Xiaodian	21				6
		双店 Shuangdian	12				3
升金湖 Shengjin Lake	Sj	升金湖 Shenjin Lake	13	0.71	0.56	6.33	5
东至 Dongzhi	Dz	香口乡 Stream in Xiangkou	21	0.74	0.33	7.55	9
望江 Wangjiang	Wj	华阳河 Huayang River	20	0.76	0.17	7.44	18
新安江 Xin'anjiang River
新安江 Xin'anjiang River	Xa	屯光镇 Tunguang	24	0.64	0.39	6.00	3
总计 Total			182	0.67	0.35	-	-

位点 Loci	重复单元Repeat unit	引物序列 Primer sequence(5'-3')	退火温度 Annealing temperature (°C)	长度 Size range (bp)	登录号 Accession no.	N_a	H_E	H_O	PIC	F_is	F_it	F_st
PclG33	(GT)₂₁	F: FAM-TTCGAGGCGTTGCTGATTGTAAGT R: CAAGGAAGCGTATAGCCGGAGTCT	56	120-180	AF290936	23	0.83	0.46	0.81	0.50	0.56	0.110
PCL29	(GA)₂₇	F: HEX-CACTCAAGCCTGCCCTCACTC R: GTCTCTTCCTCCCCCATTCTCAC	56	170-214	EF564126	14	0.87	0.44	0.85	0.55	0.55	0.108
PCL47	(TG)₁₃	F: TAMRA-ACTCTGCCCATTGTTTCTCGG R: AGCCCTTGGACCCCGCCTATC	56	324-344	EF564128	10	0.79	0.82	0.75	0.57	0.62	0.110
PCL33	(TG)₃₀	F: FAM-ACTCCTGTCCCATTTCACTAC R: ACAACTAACTGCAACTCATTCTA	56	132-158	EF564127	24	0.81	0.43	0.78	0.49	0.54	0.100
PCL28	(CA)₂₉	F: HEX-CCTACCAGAGAACCCAAAACAGAA R: GTCAGCCTCCACCACATCACTT	56	229-239	EF564125	23	0.82	0.35	0.80	0.50	0.56	0.111
PCL17	(TC)₁₇	F: TAMRA-TTCACTACCGCCCACAGGATG R: TAGGCACCGCACTTATTAGACCAG	56	370-422	EF564122	18	0.71	0.10	0.69	0.45	0.51	0.111
PclG48	(CA)₁₂	F: FAM-CTGTTGGTGATTTCCGTCAATTTT R: AGATTCAACGCTGTGTTCCTGATC	56	146-190	AF290941	22	0.81	0.24	0.79	0.47	0.53	0.108
PCL02	(TG)₃₀	F: HEX-GAAGACGGGACACCACGAG R: ATCAAATCAAACGAAGCAAGAAAG	56	245-271	EF564119	24	0.86	0.23	0.84	0.46	0.52	0.113
PCL50	(GA)₁₂	F: TAMRA-AAGCGCTGAAATGCACAAACAAGA R: CAAGCCCCGAGGTCAAAGGTC	56	424-525	EF564129	19	0.53	0.17	0.51	0.48	0.53	0.105

位点 Loci	重复单元Repeat unit	引物序列 Primer sequence(5'-3')	退火温度 Annealing temperature (°C)	长度 Size range (bp)	登录号 Accession no.	N_a	H_E	H_O	PIC	F_is	F_it	F_st
PclG33	(GT)₂₁	F: FAM-TTCGAGGCGTTGCTGATTGTAAGT R: CAAGGAAGCGTATAGCCGGAGTCT	56	120-180	AF290936	23	0.83	0.46	0.81	0.50	0.56	0.110
PCL29	(GA)₂₇	F: HEX-CACTCAAGCCTGCCCTCACTC R: GTCTCTTCCTCCCCCATTCTCAC	56	170-214	EF564126	14	0.87	0.44	0.85	0.55	0.55	0.108
PCL47	(TG)₁₃	F: TAMRA-ACTCTGCCCATTGTTTCTCGG R: AGCCCTTGGACCCCGCCTATC	56	324-344	EF564128	10	0.79	0.82	0.75	0.57	0.62	0.110
PCL33	(TG)₃₀	F: FAM-ACTCCTGTCCCATTTCACTAC R: ACAACTAACTGCAACTCATTCTA	56	132-158	EF564127	24	0.81	0.43	0.78	0.49	0.54	0.100
PCL28	(CA)₂₉	F: HEX-CCTACCAGAGAACCCAAAACAGAA R: GTCAGCCTCCACCACATCACTT	56	229-239	EF564125	23	0.82	0.35	0.80	0.50	0.56	0.111
PCL17	(TC)₁₇	F: TAMRA-TTCACTACCGCCCACAGGATG R: TAGGCACCGCACTTATTAGACCAG	56	370-422	EF564122	18	0.71	0.10	0.69	0.45	0.51	0.111
PclG48	(CA)₁₂	F: FAM-CTGTTGGTGATTTCCGTCAATTTT R: AGATTCAACGCTGTGTTCCTGATC	56	146-190	AF290941	22	0.81	0.24	0.79	0.47	0.53	0.108
PCL02	(TG)₃₀	F: HEX-GAAGACGGGACACCACGAG R: ATCAAATCAAACGAAGCAAGAAAG	56	245-271	EF564119	24	0.86	0.23	0.84	0.46	0.52	0.113
PCL50	(GA)₁₂	F: TAMRA-AAGCGCTGAAATGCACAAACAAGA R: CAAGCCCCGAGGTCAAAGGTC	56	424-525	EF564129	19	0.53	0.17	0.51	0.48	0.53	0.105

	Ch	Czh	Fx	Sj	Dz	Wj	Hf	Xa	Cd
Ch		4.00	1.56	4.08	2.59	1.63	2.86	1.52	3.13
Czh	0.06		1.67	9.49	2.52	1.62	5.75	1.38	1.92
Fx	0.14	0.13		2.89	1.23	2.00	1.71	0.86	1.27
Sj	0.06	0.03	0.08		2.72	2.58	3.97	1.27	2.03
Dz	0.09	0.09	0.17	0.08		2.66	1.54	2.72	2.26
Wj	0.13	0.13	0.11	0.09	0.09		1.80	1.92	2.41
Hf	0.08	0.04	0.13	0.06	0.14	0.12		1.11	1.57
Xa	0.14	0.15	0.23	0.16	0.08	0.12	0.18		1.69
Cd	0.07	0.12	0.16	0.11	0.10	0.09	0.14	0.13