基于ITS和ndhF-rpl32序列的荞麦种间亲缘关系分析

doi:10.17520/biods.2015259

摘要/Abstract

摘要：

荞麦起源于我国, 演化形成了丰富的物种和遗传多样性。为了有效研究和利用荞麦及其野生种资源, 以从四川、甘肃、贵州等地采集的荞麦属(Fagopyrum)10个种(含变种、亚种和复合体种)共71份材料为对象, 通过ITS和叶绿体ndhF-rpl32序列分析, 利用MEGA5.0构建系统进化树, 探讨了荞麦种内及种间亲缘关系。结果表明, 在ITS序列矩阵中, 序列长度为725 bp, 信息位点为150个, 占序列总长度的20.7%; 在ndhF-rpl32序列矩阵中, 序列长度为940 bp, 信息位点为158个, 占序列总长度的16.8%。由ITS序列和ndhF-rpl32序列构建的两个进化树都可以将71份材料分为大粒荞麦种组和小粒荞麦种组; 其中, 大粒荞麦种组包括栽培苦荞和米苦荞(F. tataricum)、金荞复合体(F. cymosum complex)、栽培甜荞(F. esculentum)和野生甜荞(F. esculentum ssp. ancestralis); 小粒荞麦种组包括齿翅野荞(F. gracilipes var. odontopterum)、疏穗小野荞(F. leptopodum var. grossii)、小野荞(F. leptopodum)、密毛野荞(F. densovillosum)、细柄野荞(F. gracilipes)和硬枝万年荞(F. urophyllum)。而ndhF-rpl32序列构建的系统发育树还能区分栽培甜荞和野生甜荞, 具有更好的聚类效果。另外, 与栽培甜荞相比, 金荞复合体与栽培苦荞的亲缘关系更近。该研究为荞麦属种的分类和条形码研究提供了一定的科学依据。

关键词: Fagopyrum, 亲缘关系, ITS, ndhF-rpl32

Abstract

Buckwheat was originated in China and evolved into abundant species and genetic diversity. In order to effectively use germplasm resources of buckwheat and its wild relatives, the genetic relationships of buckwheat species were analyzed on 71 Fagopyrum samples including 10 species (subspecies, varieties and complex), which were collected mainly from Sichuan, Guizhou and Gansu provinces. Through the analysis of ITS and ndhF-rpl32 sequences and the construction of phylogenetic trees, the inter- and intra-specific relationships of these species were discussed. Results showed that the sequence length of ITS was 725 bp in the matrix, while the parsimony informative sites was 150, accounting for 20.7% of the total length. In the matrix of ndhF-rpl32 sequence, the length was 940 bp, while the parsimony informative sites was 158, accounting for 16.8% of total length. On one hand, the phylogenetic trees based on both ITS and ndhF-rpl32 sequencing data clearly separated the big-achene-group buckwheat (F. tataricum, F. cymosum complex, F. esculentum and F. esculentum ssp. ancestralis) from the small-achene-group buckwheat (F. gracilipes var. odontopterum, F. leptopodum var. grossii, F. leptopodum, F. densovillosum, F. gracilipes, and F. urophyllum). On the other hand, the phylogenetic tree based on ndhF-rpl32 sequences can be used to distinguish F. esculentum ssp. ancestralis and F. esculentum, which indicated that the ndhF-rpl32 sequence was more effective in analyzing the phylogenetic relationships of buckwheat species. These results provide a scientific basis for studies of taxonomy and establishing barcodes of Fagopyrum species.

Key words: buckwheat, genetic relationships, ITS, ndhF-rpl32

胡亚妮, 张宗文, 吴斌, 高佳, 李艳琴 (2016) 基于ITS和ndhF-rpl32序列的荞麦种间亲缘关系分析. 生物多样性, 24, 296-303. DOI: 10.17520/biods.2015259.

Yani Hu, Zongwen Zhang, Bin Wu, Jia Gao, Yanqin Li (2016) Genetic relationships of buckwheat species based on the sequence analysis of ITS and ndhF-rpl32. Biodiversity Science, 24, 296-303. DOI: 10.17520/biods.2015259.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.biodiversity-science.net/CN/10.17520/biods.2015259

https://www.biodiversity-science.net/CN/Y2016/V24/I3/296

图/表 5

参考文献 27

1	Chen QF (1999a) A study of resources of Fagopyrum (Polygonaceae) native to China. Botanical Journal of the Linnean Society, 130, 53-64.
2	Chen QF (1999b) Hybridization between Fagopyrum (Polygonaceae) species native to China. Botanical Journal of the Linnean Society, 131, 177-185.
3	Chen QF (2004) A study of isozyme and interspecific hybridization on big-achene group of buckwheat species (Fagopyrum, Polygonaceae). Crop Sciences, 44, 1151-1158.
4	Chen QF (2012) Plant Sciences on Genus Fagopyrum. Science Press, Beijing.(in Chinese)
	[陈庆富 (2012) 荞麦属植物科学. 科学出版社, 北京.]
5	Doyle JJ (1986) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19, 11-15.
6	Han JP, Song JY, Liu C, Chen J, Qian J, Zhu YJ, Shi LC, Yao H, Chen SL (2010) Identification of Cistanche species (Orobanchaceae) based on sequences of the plastid psbA- trnH intergenic region. Acta Pharmaceutica Sinica, 45, 126-130.
7	Hedberg O (1946) Pollen morphology in the genus Polygonees L. (s. lat.) and its taxonomical significance. Svensk Botaisk Tidskrift, 40, 371-404.
8	Hirose T, Ujihara A, Kitayashi H, Minami M (1995) Pollen tube behavior related to self incompatibility in interspecific crosses of Fagopyrum. Breeding Science, 45, 65-70.
9	Hou YJ, Zhang ZW, Wu B, Li YQ (2009) Genetic diversity in tartary buckwheat revealed by AFLP analysis. Scientia Agricultura Sinica, 42, 4166-4174.(in Chinese with English abstract)
	[侯雅君, 张宗文, 吴斌, 李艳琴 (2009) 苦荞种质资源AFLP标记遗传多样性分析. 中国农业科学, 42, 4166-4174.]
10	Liu J, He T, Chun Z (2009) Analysis and authentication of chloroplast matK gene sequences of Herba dendrobii. Acta Pharmaceutica Sinica, 44, 1051-1055.(in Chinese with English abstract)
	[刘静, 何涛, 淳泽 (2009) 药用石斛的叶绿体matK基因序列分析及鉴别. 药学学报, 44, 1051-1055.]
11	Ohsako T, Ohishi O (2000) Intra and interspecific phylogeny of wild Fagopyrum (Polygonaceae) species based on nucleotide sequences of noncoding regions in chloroplast DNA. American Journal of Botany, 87, 573-582.
12	Sharma TR, Jana S (2002a) Random amplified polymorphic DNA (RAPD) variation in Fagopyrum tataricum Gaertn. accessions from China and the Himalayan region. Euphytica, 127, 327-333.
13	Sharma TR, Jana S (2002b) Species relationships in Fagopyrum revealed by PCR based DNA fingerprinting. Theoretical & Applied Genetics, 105, 306-312.
14	Shevchuk TE, Gavrilyuk IP, Konarev VG (1981) Immunochemical analysis of the affinities of the genus Fagopyrum and the species of some other genera of the family Polygonaceae. Botanical Journal, 66, 259-267.
15	Shi JQ, Li YQ, Zhang ZW, Wu B, Wang AH (2015) Genetic diversity of buckwheat and its wild species. Journal of Plant Genetic Resources, 16, 443-450.(in Chinese with English abstract)
	[史建强, 李艳琴, 张宗文, 吴斌, 王安虎 (2015) 荞麦及其野生种遗传多样性分析. 植物遗传资源学报, 16, 443-450.]
16	Steward AN (1930) The Polygonaceae of Eastern Asia. Contributions from the Gray Herbarium of Harvard University, Boston.
17	Tsuji K, Ohnishi O (2001) Phylogenetic position of east Tibetan natural populations in Tartary buckwheat (Fagopyrum tataricum Gaertn.) revealed by RAPD analyses. Genetic Resources and Crop Evolution, 48, 63-67.
18	Wang AH, Xia MZ, Cai GZ, Ren YH (2006) Study of the characteristics and geographical distribution of wild buckwheat resources in eastern Liangshan of Sichuan Province. Crops, (5), 25-27.(in Chinese)
	[王安虎, 夏明忠, 蔡光泽, 任迎虹 (2006) 四川省凉山州东部野生荞麦资源的特征特性和地理分布研究. 作物杂志, (5), 25-27.]
19	Wang AH, Xia MZ, Cai GZ, Yang P (2008a) The origin of cultivating buckwheat and the genetic analysis of the kindred species. Southwest China Journal of Agricultural Sciences, 21, 282-285.(in Chinese with English abstract)
	[王安虎, 夏明忠, 蔡光泽, 杨坪 (2008a) 栽培苦荞麦的起源及其近缘种亲缘分析. 西南农业学报, 21, 282-285.]
20	Wang AH, Xia MZ, Cai GZ, Ren YH (2008b) Studies on the characteristics and the geographical distribution diversity of wild buckwheat resources in Sichuan. Southwest China Journal of Agricultural Sciences, 21, 575-580.(in Chinese with English abstract)
	[王安虎, 夏明忠, 蔡光泽, 任迎虹 (2008b) 四川野生荞麦资源的特征特性与地理分布多样性研究. 西南农业学报, 21, 575-580.]
21	Wang LH, Yin FY, Liu JM, Ye CR (2004) Genetic diversity and relationships of the wild buckwheat resources from Yunnan Province by RAPD. Molecular Plant Breeding, 2, 807-815.(in Chinese with English abstract)
	[王莉花, 殷富有, 刘继梅, 叶昌荣 (2004) 利用RAPD分析云南野生荞麦资源的多样性和亲缘关系. 分子植物育种, 2, 807-815.]
22	White TJ, Bruns T, Lee S (1990) Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. Academic Press, San Diego.
23	Xu LP (2014) Research progress of nutrient and chemical constituents in buckwheat. Sichuan Chemical Industry, 17(4), 4-8.(in Chinese with English abstract)
	[徐珑珀 (2014) 荞麦营养与化学成份研究进展. 四川化工, 17(4), 4-8.]
24	Yang YX (2008) Studies on Genetic Diversity of Buckwheat Germplasms. PhD dissertation, Sichuan Agricultural University, Chengdu.(in Chinese with English abstract)
	[杨玉霞 (2008) 荞麦种质资源遗传多样性研究. 博士学位论文, 四川农业大学, 雅安]
25	Yang XY, Chen H, Shao JR, Wu Q, Tang Y (2007) Preliminary study on genetic relationship among buckwheat species in north western Sichuan. Acta Botanica Boreali-Occidentalia Sinica, 27, 1752-1758.(in Chinese with English abstract)
	[杨小艳, 陈惠, 邵继荣, 吴琦, 唐宇 (2007) 川西北荞麦种间亲缘关系初步研究. 西北植物学报, 27, 1752-1758.]
26	Yu H, Mao J, Qu SP, Xiong L, Li YY, He R (2005) Study on the relationship of nine varieties of Asian Lilium based on nrDNA ITS. Southwest China Journal of Agricultural Sciences, 18, 387-391.(in Chinese with English abstract)
	[虞泓, 毛钧, 瞿素萍, 熊丽, 李永谊, 和锐 (2005) 亚洲系百合九个品种的亲缘关系研究——来自nrDNA ITS证据. 西南农业学报, 18, 387-391.]
27	Zhang L, Gao FH, Gao LJ, Yin XM, Diao Y (2011) Research progress of buckwheat nutrition, function and its products. South China Agriculture, 5(6), 74-77.(in Chinese)
	[张玲, 高飞虎, 高伦江, 尹旭敏, 刁源 (2011) 荞麦营养功能及其利用研究进展. 南方农业, 5(6), 74-77.]

编号 Collecting number	原产地 Origin	代号 Code	编号 Collecting number	原产地 Origin	代号 Code
齿翅野荞 Fagopyrum gracilipes var. odontopterum			细柄野荞 F. gracilipes
YXCC201211030	四川越西 Yuexi, Sichuan	a1	XDXB201211028	四川喜德 Xide, Sichuan	g1
ZJCC2012102601	四川昭觉 Zhaojue, Sichuan	a2	ZJXB2012102602	四川昭觉 Zhaojue, Sichuan	g2
ZJCC2012102603	四川昭觉 Zhaojue, Sichuan	a3	ZJXB2012102605	四川昭觉 Zhaojue, Sichuan	g3
ZJCC2012102604	四川昭觉 Zhaojue, Sichuan	a4	ZJXB2012102703	四川昭觉 Zhaojue, Sichuan	g4
ZJCC2012102704	四川昭觉 Zhaojue, Sichuan	a5	MGXB2012102901	四川美姑 Meigu, Sichuan	g5
BTCC2012102706	四川布拖 Butuo, Sichuan	a6	HLXB2012110303	四川会理 Huili, Sichuan	g6
ZJCC2012102801	四川昭觉 Zhaojue, Sichuan	a7	HLXB2012110306	四川会理 Huili, Sichuan	g7
MGCC2012102902	四川美姑 Meigu, Sichuan	a8	HDXB2012110404	四川会东 Huidong, Sichuan	g8
LBCC2012103002	四川雷波 Leibo, Sichuan	a9	HDXB2012110501	四川会东 Huidong, Sichuan	g9
HLCC2012110304	四川会理 Huili, Sichuan	a10	HDCC2012110504	四川会东 Huidong, Sichuan	g10
HLCC2012110305	四川会理 Huili, Sichuan	a11	HDXB2012110504	四川会东 Huidong, Sichuan	g11
HDCC2012110502	四川会东 Huidong, Sichuan	a12	HLXB2012110301	四川会理 Huili, Sichuan	g12
HDCC2012110503	四川会东 Huidong, Sichuan	a13	MNXB201210006	四川冕宁 Mianning, Sichuan	g13
YXCC201211040	四川越西 Yuexi, Sichuan	a14	XDXB201210014	四川喜德 Xide, Sichuan	g14
YXCC201211044	四川越西 Yuexi, Sichuan	a15	XDXB201211046	四川喜德 Xide, Sichuan	g15
YYCC201210012	四川盐源 Yanyuan, Sichuan	a16	YXXB201211031	四川越西 Yuexi, Sichuan	g16
HDCC2012110402	四川越西 Yuexi, Sichuan	a17	金荞复合体 F. cymosum complex
栽培苦荞 F. tataricum			XDJQ201210004	四川喜德 Xide, Sichuan	h1
XDKQ201210003	四川喜德 Xide, Sichuan	b1	MNJQ201210007	四川冕宁 Mianning, Sichuan	h2
XDKQ201210005	四川喜德 Xide, Sichuan	b2	XDKQ201210017	西川喜德 Xide, Sichuan	h3
XDKQ201210013	四川喜德 Xide, Sichuan	b3	XDJQ201210022	西川喜德 Xide, Sichuan	h4
XDKQ201210019	四川喜德 Xide, Sichuan	b4	YXJQ201211029	四川越西 Yuexi, Sichuan	h5
XDKQ201210020	四川喜德 Xide, Sichuan	b5	YXJQ201211039	四川越西 Yuexi, Sichuan	h6
YXKQ201211032	四川越西 Yuexi, Sichuan	b6	XDJQ201211045	四川喜德 Xide, Sichuan	h7
YXKQ201211033	四川越西 Yuexi, Sichuan	b7	DCJQ2012110302	四川德昌 Dechang, Sichuan	h8
疏穗小野荞 F. leptopodum var.grossii			PGJQ2012110601	四川普格 Puge, Sichuan	h9
GLSS201211037	四川甘洛 Ganluo, Sichuan	c1	DCJQ2012110202	四川德昌 Dechang, Sichuan	h10
GLSS201211038	四川甘洛 Ganluo, Sichuan	c2	HDJQ2012110403	四川会东 Huidong, Sichuan	h11
GLSS201211050	四川甘洛 Ganluo, Sichuan	c3	XDKQ201210018	四川喜德 Xide, Sichuan	h12
GLSS201211052	四川甘洛 Ganluo, Sichuan	c4	YXJQ201211043	四川越西 Yuexi, Sichuan	h13
YXSS201211051	四川越西 Yuexi, Sichuan	c5	硬枝万年荞 F. urophyllum
野生甜荞 F. esculentum ssp. ancestralis			YYYZ201210011	四川盐源 Yanyuan, Sichuan	i1
XCYTQ201210008	四川西昌 Xichang, Sichuan	d1	LBYZ2012103004	四川雷波 Leibo, Sichuan	i2
LBTJ2012103001	四川雷波 Leibo, Sichuan	d2	MGYZ2012102903	四川美姑 Meigu, Sichuan	i3
小野荞 F. leptopodum			栽培甜荞 F. esculentum
XDXY201210021	四川喜德 Xide, Sichuan	e1	00000664	甘肃武威 Wuwei, Gansu	j1
MNXY201210024	四川冕宁 Mianning, Sichuan	e2	00000906	贵州威宁 Weining, Guizhou	j2
密毛野荞 F. densovillosum			米苦荞 F. tataricum
MGMM2012102804	四川美姑 Meigu, Sichuan	f1	YXXMQ201211034	四川越西 Yuexi, Sichuan	k1
LBMM2012103003	四川雷波 Leibo, Sichuan	f2	YXXMQ201211042	四川越西 Yuexi, Sichuan	k2

编号 Collecting number	原产地 Origin	代号 Code	编号 Collecting number	原产地 Origin	代号 Code
齿翅野荞 Fagopyrum gracilipes var. odontopterum			细柄野荞 F. gracilipes
YXCC201211030	四川越西 Yuexi, Sichuan	a1	XDXB201211028	四川喜德 Xide, Sichuan	g1
ZJCC2012102601	四川昭觉 Zhaojue, Sichuan	a2	ZJXB2012102602	四川昭觉 Zhaojue, Sichuan	g2
ZJCC2012102603	四川昭觉 Zhaojue, Sichuan	a3	ZJXB2012102605	四川昭觉 Zhaojue, Sichuan	g3
ZJCC2012102604	四川昭觉 Zhaojue, Sichuan	a4	ZJXB2012102703	四川昭觉 Zhaojue, Sichuan	g4
ZJCC2012102704	四川昭觉 Zhaojue, Sichuan	a5	MGXB2012102901	四川美姑 Meigu, Sichuan	g5
BTCC2012102706	四川布拖 Butuo, Sichuan	a6	HLXB2012110303	四川会理 Huili, Sichuan	g6
ZJCC2012102801	四川昭觉 Zhaojue, Sichuan	a7	HLXB2012110306	四川会理 Huili, Sichuan	g7
MGCC2012102902	四川美姑 Meigu, Sichuan	a8	HDXB2012110404	四川会东 Huidong, Sichuan	g8
LBCC2012103002	四川雷波 Leibo, Sichuan	a9	HDXB2012110501	四川会东 Huidong, Sichuan	g9
HLCC2012110304	四川会理 Huili, Sichuan	a10	HDCC2012110504	四川会东 Huidong, Sichuan	g10
HLCC2012110305	四川会理 Huili, Sichuan	a11	HDXB2012110504	四川会东 Huidong, Sichuan	g11
HDCC2012110502	四川会东 Huidong, Sichuan	a12	HLXB2012110301	四川会理 Huili, Sichuan	g12
HDCC2012110503	四川会东 Huidong, Sichuan	a13	MNXB201210006	四川冕宁 Mianning, Sichuan	g13
YXCC201211040	四川越西 Yuexi, Sichuan	a14	XDXB201210014	四川喜德 Xide, Sichuan	g14
YXCC201211044	四川越西 Yuexi, Sichuan	a15	XDXB201211046	四川喜德 Xide, Sichuan	g15
YYCC201210012	四川盐源 Yanyuan, Sichuan	a16	YXXB201211031	四川越西 Yuexi, Sichuan	g16
HDCC2012110402	四川越西 Yuexi, Sichuan	a17	金荞复合体 F. cymosum complex
栽培苦荞 F. tataricum			XDJQ201210004	四川喜德 Xide, Sichuan	h1
XDKQ201210003	四川喜德 Xide, Sichuan	b1	MNJQ201210007	四川冕宁 Mianning, Sichuan	h2
XDKQ201210005	四川喜德 Xide, Sichuan	b2	XDKQ201210017	西川喜德 Xide, Sichuan	h3
XDKQ201210013	四川喜德 Xide, Sichuan	b3	XDJQ201210022	西川喜德 Xide, Sichuan	h4
XDKQ201210019	四川喜德 Xide, Sichuan	b4	YXJQ201211029	四川越西 Yuexi, Sichuan	h5
XDKQ201210020	四川喜德 Xide, Sichuan	b5	YXJQ201211039	四川越西 Yuexi, Sichuan	h6
YXKQ201211032	四川越西 Yuexi, Sichuan	b6	XDJQ201211045	四川喜德 Xide, Sichuan	h7
YXKQ201211033	四川越西 Yuexi, Sichuan	b7	DCJQ2012110302	四川德昌 Dechang, Sichuan	h8
疏穗小野荞 F. leptopodum var.grossii			PGJQ2012110601	四川普格 Puge, Sichuan	h9
GLSS201211037	四川甘洛 Ganluo, Sichuan	c1	DCJQ2012110202	四川德昌 Dechang, Sichuan	h10
GLSS201211038	四川甘洛 Ganluo, Sichuan	c2	HDJQ2012110403	四川会东 Huidong, Sichuan	h11
GLSS201211050	四川甘洛 Ganluo, Sichuan	c3	XDKQ201210018	四川喜德 Xide, Sichuan	h12
GLSS201211052	四川甘洛 Ganluo, Sichuan	c4	YXJQ201211043	四川越西 Yuexi, Sichuan	h13
YXSS201211051	四川越西 Yuexi, Sichuan	c5	硬枝万年荞 F. urophyllum
野生甜荞 F. esculentum ssp. ancestralis			YYYZ201210011	四川盐源 Yanyuan, Sichuan	i1
XCYTQ201210008	四川西昌 Xichang, Sichuan	d1	LBYZ2012103004	四川雷波 Leibo, Sichuan	i2
LBTJ2012103001	四川雷波 Leibo, Sichuan	d2	MGYZ2012102903	四川美姑 Meigu, Sichuan	i3
小野荞 F. leptopodum			栽培甜荞 F. esculentum
XDXY201210021	四川喜德 Xide, Sichuan	e1	00000664	甘肃武威 Wuwei, Gansu	j1
MNXY201210024	四川冕宁 Mianning, Sichuan	e2	00000906	贵州威宁 Weining, Guizhou	j2
密毛野荞 F. densovillosum			米苦荞 F. tataricum
MGMM2012102804	四川美姑 Meigu, Sichuan	f1	YXXMQ201211034	四川越西 Yuexi, Sichuan	k1
LBMM2012103003	四川雷波 Leibo, Sichuan	f2	YXXMQ201211042	四川越西 Yuexi, Sichuan	k2

种名 Species	ITS (%)	ndhF-rpl32 (%)
齿翅野荞 F. gracilipes var. odontopterum	65.57	24.17
栽培苦荞 F. tataricum	67.65	22.79
疏穗小野荞 F. leptopodum var. grossii	65.96	22.01
野生甜荞 F. esculentum ssp. ancestralis	66.46	24.47
小野荞 F. leptopodum	66.82	23.43
密毛野荞 F. densovillosum	65.34	24.77
细柄野荞 F. gracilipes	65.81	23.17
金荞复合体 F. cymosum complex	65.80	22.75
硬枝万年荞 F. urophyllum	63.93	24.68
栽培甜荞 F. esculentum	65.47	24.13
米苦荞 F. tataricum	67.95	23.40

种名 Species	ITS (%)	ndhF-rpl32 (%)
齿翅野荞 F. gracilipes var. odontopterum	65.57	24.17
栽培苦荞 F. tataricum	67.65	22.79
疏穗小野荞 F. leptopodum var. grossii	65.96	22.01
野生甜荞 F. esculentum ssp. ancestralis	66.46	24.47
小野荞 F. leptopodum	66.82	23.43
密毛野荞 F. densovillosum	65.34	24.77
细柄野荞 F. gracilipes	65.81	23.17
金荞复合体 F. cymosum complex	65.80	22.75
硬枝万年荞 F. urophyllum	63.93	24.68
栽培甜荞 F. esculentum	65.47	24.13
米苦荞 F. tataricum	67.95	23.40

种名 Species	ITS	ndhF-rpl32
齿翅野荞 F. gracilipes var. odontopterum	0.001	0.182
栽培苦荞 F. tataricum	0.003	0.000
疏穗小野荞 F. leptopodum var. grossii	0.002	0.002
野生甜荞 F. esculentum ssp. ancestralis	0.107	0.001
小野荞 F. leptopodum	0.062	0.021
密毛野荞 F. densovillosum	0.003	0.018
细柄野荞 F. gracilipes	0.001	0.099
金荞复合体 F. cymosum complex	0.028	0.026
硬枝万年荞 F. urophyllum	0.165	0.014
栽培甜荞 F. esculentum	0.110	0.000
米苦荞 F. tataricum	0.000	0.001