雪峰乌骨鸡自然群体遗传多样性的微卫星分析

doi:10.3724/SP.J.1003.2008.07320

生物多样性 ›› 2008, Vol. 16 ›› Issue (5): 503-508. DOI: 10.3724/SP.J.1003.2008.07320

雪峰乌骨鸡自然群体遗传多样性的微卫星分析

魏麟¹^,^#, 刘胜贵², 史宪伟³^,^*()

1 湖南农业大学动物科学技术学院, 长沙 410128
2 湖南怀化学院生物工程系, 怀化 418008
3 云南农业大学动物科学技术学院, 云南省生物多样性与生物技术人才培养基地, 昆明 650201

收稿日期:2007-10-31 接受日期:2008-04-01 出版日期:2008-09-20 发布日期:2008-09-20
通讯作者: 史宪伟
基金资助:
湖南省自然科学基金(07JJ6044);湖南省教育厅资助科研项目(06C635)

Genetic diversity of the Xuefeng black bone chicken based on microsatellite markers

Lin Wei¹^,^#, Shenggui Liu², Xianwei Shi³^,^*()

1 College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128
2 The Department of Biological Engineering in Huaihua College, Huaihua 418008
3 College of Animal Science and Technology, Yunnan Agriculture University; The Center for Agricultural Biodiversity and Biotechnology Research and Training of Yunnan Province, Kunming 650201

Received:2007-10-31 Accepted:2008-04-01 Online:2008-09-20 Published:2008-09-20
Contact: Xianwei Shi

摘要/Abstract

摘要：

雪峰乌骨鸡是具有独特遗传特性的优质肉药兼用地方家禽品种。为了评价雪峰乌骨鸡现有品种资源的遗传背景、群体遗传结构等, 提出合理的保种方法和措施, 本研究筛选了家鸡基因组23条染色体上的23个微卫星标记, 对采自湖南怀化的50只纯种乌骨鸡个体进行了多态性检测。结果表明: 23个微卫星座位共检测到79个等位基因, 所有座位都呈现出多态性, 每个座位的等位基因数在2-6个之间, 平均为3.435个。群体表现出较高的平均杂合度和平均多态信息含量, 分别为0.6285和0.5496, 说明雪峰乌骨鸡自然群体具有较丰富的遗传多样性。研究结果将为雪峰乌骨鸡种的保护和利用提供重要的理论依据。

关键词: 雪峰乌骨鸡, 微卫星标记, 多态信息含量, 杂合度

Abstract

We studied polymorphisms of 23 microsatellite loci from 23 chromosomes in an indigenous breed of Xuefeng black bone chicken from Hunan in order to elucidate its genetic diversity and structure and to propose reasonable measures for its conservation and utilization. Seventy-nine alleles were identified in 50 individuals and the mean number of alleles was 3.435 per locus. Average heterozygosity and polymorphic information content of 23 microsatellite loci were 0.6285 and 0.5496, respectively. These results indicate that this breed of Xuefeng black bone chicken has a high level of genetic diversity and may provide a scientific basis for future conservation and utilization of the Xuefeng black bone chicken.

Key words: Xuefeng black bone chicken, microsatellite markers, polymorphism information content, heterozygosity

魏麟, 刘胜贵, 史宪伟 (2008) 雪峰乌骨鸡自然群体遗传多样性的微卫星分析. 生物多样性, 16, 503-508. DOI: 10.3724/SP.J.1003.2008.07320.

Lin Wei, Shenggui Liu, Xianwei Shi (2008) Genetic diversity of the Xuefeng black bone chicken based on microsatellite markers. Biodiversity Science, 16, 503-508. DOI: 10.3724/SP.J.1003.2008.07320.

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链接本文: https://www.biodiversity-science.net/CN/10.3724/SP.J.1003.2008.07320

https://www.biodiversity-science.net/CN/Y2008/V16/I5/503

图/表 3

参考文献 21

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采集地点 Collection sites	数量 Sample size	采集地点 Collection sites	数量 Sample size
雪峰镇界脚村 Jiejiao Village, Xuefeng Town	3	塘湾镇白羊村 Baiyang Village, Tangwan Town	3
雪峰镇车力溪村 Chelixi Village, Xuefeng Town	2	塘湾镇木兰溪村 Mulanxi Village, Tangwan Town	3
雪峰镇桐溪村 Tongxi Village, Xuefeng Town	2	塘湾镇里木冲村 Limuchong Village, Tangwan Town	3
雪峰镇先峰溪村 Xianfengxi Village, Xuefeng Town	2	安江镇倒水湾村 Daoshuiwan Village, Anjiang Town	3
雪峰镇两溪口村 Liangxikou Village, Xuefeng Town	2	安江镇桃子垅村 Taozilong Village, Anjiang Town	3
雪峰镇龙家田村 Longjiatian Village, Xuefeng Town	2	安江镇油菜园村 Youcaiyuan Village, Anjiang Town	3
雪峰镇兰溪冲村 Lanxichong Village, Xuefeng Town	2	安江镇大沙坪村 Dashaping Village, Anjiang Town	2
雪峰镇青山洞村 Qingshandong Village, Xuefeng Town	2	托口镇王家坳村 Wangjia’ao Village, Tuokou Town	3
雪峰镇笙竹村 Shengzhu Village, Xuefeng Town	2	硖州乡下坪村 Xiaping Village, Xiazhou Town	2
塘湾镇永红村 Yonghong Village, Tangwan Town	4	硖州乡溪边村 Xibian Village, Xiazhou Town	2

采集地点 Collection sites	数量 Sample size	采集地点 Collection sites	数量 Sample size
雪峰镇界脚村 Jiejiao Village, Xuefeng Town	3	塘湾镇白羊村 Baiyang Village, Tangwan Town	3
雪峰镇车力溪村 Chelixi Village, Xuefeng Town	2	塘湾镇木兰溪村 Mulanxi Village, Tangwan Town	3
雪峰镇桐溪村 Tongxi Village, Xuefeng Town	2	塘湾镇里木冲村 Limuchong Village, Tangwan Town	3
雪峰镇先峰溪村 Xianfengxi Village, Xuefeng Town	2	安江镇倒水湾村 Daoshuiwan Village, Anjiang Town	3
雪峰镇两溪口村 Liangxikou Village, Xuefeng Town	2	安江镇桃子垅村 Taozilong Village, Anjiang Town	3
雪峰镇龙家田村 Longjiatian Village, Xuefeng Town	2	安江镇油菜园村 Youcaiyuan Village, Anjiang Town	3
雪峰镇兰溪冲村 Lanxichong Village, Xuefeng Town	2	安江镇大沙坪村 Dashaping Village, Anjiang Town	2
雪峰镇青山洞村 Qingshandong Village, Xuefeng Town	2	托口镇王家坳村 Wangjia’ao Village, Tuokou Town	3
雪峰镇笙竹村 Shengzhu Village, Xuefeng Town	2	硖州乡下坪村 Xiaping Village, Xiazhou Town	2
塘湾镇永红村 Yonghong Village, Tangwan Town	4	硖州乡溪边村 Xibian Village, Xiazhou Town	2

座位 Locus	上游引物序列 Forward primer’s sequences(5’→3’)
ADL0188	CACTTCCAGTATTAACGTGA	GTGGACACAATGAGTTCCTC	54	125-209	1
ADL0190	TCAGCTCTTCAGGCAAAAAG	AACTTGGACCACAATCTTAT	52	220-231	2
MCW0224	ATTACCTTTCTTCATTAACGCC	TTCATAGACTTGAGCGAGGAC	56	291-301	3
MCW0170	TTGTGAAACTCACAGCAGCTG	TTATAGCAGGCTGGCCTGAAG	60	263-267	4
MCW0029	CATGCAATTCAGGACCGTGCA	GTGGACACCCATTTGTACCCTATG	56	149-194	5
MCW0176	AAAGAGAAGTATAAAACATGCC	TCCATTCTTGGCAGTGCATAG	58	257-270	6
MCW0120	CTATGTAAAGCTTGAATCTTCA	ATTCCTGGGTGCTAATTTACC	54	250-287	7
ADL0121	CTGGAACAAGAGGGCTTTGC	GGATGTGAAAAATCTCCTGG	56	125-157	8
MCW0134	GGAGACTTCATTGTGTAGCAC	ACCAAAAGACTGGAGGTCAAC	56	260-284	9
MCW0035	CAGAAACATTTGGACTTGGCTT	TTGCTTCATTTCTAGTCTCCAGTT	60	227-233	10
MCW0097	GGAGAGCATCTGCCTTCCTAG	TGGTCTTCCAGTCTATGGTAG	56	263-309	11
MCW0198	GATCTTTGCTACCATCCACTG	ACCCATCTGGTTGGACTATGC	58	97-152	12
MCW0104	TAGCACAACTCAAGCTGTGAG	AGACTTGCACAGCTGTGTACC	56	189-263	13
LEI0098	AAAAGACAATGCAATTGGTGC	CTGCCACTGATGCTGTCACT	60	147-170	14
MCW0080	GAAATGGTACAGTGCAGTTGG	CCGTGCATTCTTAATTGACAG	58	278-337	15
MCW0330	TGGACCTCATCAGTCTGACAG	AATGTTCTCATAGAGTTCCTGC	56	260-290	17
MCW0217	GATCTTTCTGGAACAGATTTC	CTGCACTTGGTTCAGGTTCTG	56	153-174	18
MCW0094	GGAGCTGGTATTTGTCCTAAG	GCACAGCCTTTTGACATGTAC	60	77-95	19
MCW0165	CAGACATGCATGCCCAGATGA	GATCCAGTCCTGCAGGCTGC	55	125-144	23
MCW0285	AGTTGGAGGTTATATTA CGGG	TATGACATAATCCACGCTGAG	58	156-300	26
MCW0328	ATGGAAACAGATGGAGCTGGC	CTCCAATCCCAGGCTCCAAC	57	262-324	27
ADL0284	CAGAGTTCATCCGCCACTGC	CCTCCCCACTAACATTGGAA	60	137-167	28
LEI0254	AGACCACTGGATCCAACTC	GTCTGGAACTCATCCCTTCATC	55	89-101	Z

座位 Locus	上游引物序列 Forward primer’s sequences(5’→3’)
ADL0188	CACTTCCAGTATTAACGTGA	GTGGACACAATGAGTTCCTC	54	125-209	1
ADL0190	TCAGCTCTTCAGGCAAAAAG	AACTTGGACCACAATCTTAT	52	220-231	2
MCW0224	ATTACCTTTCTTCATTAACGCC	TTCATAGACTTGAGCGAGGAC	56	291-301	3
MCW0170	TTGTGAAACTCACAGCAGCTG	TTATAGCAGGCTGGCCTGAAG	60	263-267	4
MCW0029	CATGCAATTCAGGACCGTGCA	GTGGACACCCATTTGTACCCTATG	56	149-194	5
MCW0176	AAAGAGAAGTATAAAACATGCC	TCCATTCTTGGCAGTGCATAG	58	257-270	6
MCW0120	CTATGTAAAGCTTGAATCTTCA	ATTCCTGGGTGCTAATTTACC	54	250-287	7
ADL0121	CTGGAACAAGAGGGCTTTGC	GGATGTGAAAAATCTCCTGG	56	125-157	8
MCW0134	GGAGACTTCATTGTGTAGCAC	ACCAAAAGACTGGAGGTCAAC	56	260-284	9
MCW0035	CAGAAACATTTGGACTTGGCTT	TTGCTTCATTTCTAGTCTCCAGTT	60	227-233	10
MCW0097	GGAGAGCATCTGCCTTCCTAG	TGGTCTTCCAGTCTATGGTAG	56	263-309	11
MCW0198	GATCTTTGCTACCATCCACTG	ACCCATCTGGTTGGACTATGC	58	97-152	12
MCW0104	TAGCACAACTCAAGCTGTGAG	AGACTTGCACAGCTGTGTACC	56	189-263	13
LEI0098	AAAAGACAATGCAATTGGTGC	CTGCCACTGATGCTGTCACT	60	147-170	14
MCW0080	GAAATGGTACAGTGCAGTTGG	CCGTGCATTCTTAATTGACAG	58	278-337	15
MCW0330	TGGACCTCATCAGTCTGACAG	AATGTTCTCATAGAGTTCCTGC	56	260-290	17
MCW0217	GATCTTTCTGGAACAGATTTC	CTGCACTTGGTTCAGGTTCTG	56	153-174	18
MCW0094	GGAGCTGGTATTTGTCCTAAG	GCACAGCCTTTTGACATGTAC	60	77-95	19
MCW0165	CAGACATGCATGCCCAGATGA	GATCCAGTCCTGCAGGCTGC	55	125-144	23
MCW0285	AGTTGGAGGTTATATTA CGGG	TATGACATAATCCACGCTGAG	58	156-300	26
MCW0328	ATGGAAACAGATGGAGCTGGC	CTCCAATCCCAGGCTCCAAC	57	262-324	27
ADL0284	CAGAGTTCATCCGCCACTGC	CCTCCCCACTAACATTGGAA	60	137-167	28
LEI0254	AGACCACTGGATCCAACTC	GTCTGGAACTCATCCCTTCATC	55	89-101	Z

座位 Locus	等位基因频率 Allele frequencies	等位基因数 No. of allele	杂合度 Heterozygosity	多态信息含量 PIC
座位 Locus	P1	P2	P3	P4	P5	P6
ADL0188	0.3166	0.2167	0.2000	0.2667			4	0.7417	0.6939
ADL0190	0.5167	0.4833					2	0.4994	0.3747
MCW0224	0.5714	0.3878	0.0408				3	0.5214	0.4211
MCW0170	0.1500	0.2167	0.5000	0.1333			4	0.6628	0.5690
MCW0029	0.5000	0.5000					2	0.5000	0.3750
MCW0176	0.2333	0.2167	0.5500				3	0.5961	0.5342
MCW0120	0.2800	0.2400	0.1200	0.1500	0.1500	0.0700	6	0.8026	0.7738
ADL0121	0.2414	0.3793	0.3793				3	0.7110	0.6530
MCW0134	0.3500	0.6500					2	0.4550	0.3515
MCW0035	0.2333	0.4833	0.2833				3	0.6317	0.5434
MCW0097	0.0667	0.2667	0.3176	0.3500			4	0.7016	0.6463
MCW0198	0.2500	0.2667	0.4833				3	0.6328	0.5412
MCW0104	0.2755	0.2245	0.2245	0.2755			4	0.7474	0.7015
LEI0098	0.1167	0.1833	0.7000				3	0.4628	0.3836
MCW0080	0.3167	0.4667	0.2166				3	0.6439	0.5846
MCW0330	0.2245	0.2551	0.2857	0.1837	0.0510		5	0.7666	0.7227
MCW0217	0.1600	0.4000	0.4400				3	0.6208	0.5390
MCW0094	0.4285	0.5715					2	0.4899	0.3700
MCW0165	0.5300	0.0800	0.1600	0.0600	0.1700		5	0.6546	0.5902
MCW0285	0.4500	0.2833	0.2667				3	0.6461	0.5771
MCW0328	0.5000	0.5000					2	0.5000	0.3750
ADL0284	0.2813	0.2604	0.0417	0.0208	0.1875	0.2083	6	0.7723	0.7163
LEI0254	0.1333	0.1333	0.3667	0.3667			4	0.6955	0.6041
群体平均数及标准误 Mean and standard error (Mean±SE)							3.4350±0.2501	0.6285±0.0225	0.5496±0.0276

雪峰乌骨鸡自然群体遗传多样性的微卫星分析

Genetic diversity of the Xuefeng black bone chicken based on microsatellite markers

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