运用PCR-RFLP方法研究三亚鹿回头岸礁造礁石珊瑚共生藻的组成

doi:10.3724/SP.J.1003.2008.08074

生物多样性 ›› 2008, Vol. 16 ›› Issue (5): 498-502. DOI: 10.3724/SP.J.1003.2008.08074 cstr: 32101.14.SP.J.1003.2008.08074

运用PCR-RFLP方法研究三亚鹿回头岸礁造礁石珊瑚共生藻的组成

董志军¹^,²^,³, 黄晖¹^,^*(), 黄良民¹, 李元超¹, 李秀保¹

1 中国科学院南海海洋研究所海洋生物资源可持续利用重点实验室, 广州 510301
2 中国科学院研究生院, 北京 100049
3 中国科学院海南热带海洋生物实验站, 三亚 572000

收稿日期:2008-04-02 接受日期:2008-05-21 出版日期:2008-09-20 发布日期:2008-09-20
通讯作者: 黄晖
基金资助:
中科院台站基金支持项目(YTZJJ0502);国家海洋局908专项资助项目(908-ST-01-08-珊瑚礁调查);国家自然科学基金(30200039);国家自然科学基金(90211015);国家自然科学基金(40776085);国家自然科学基金(40576052);国家基金委–广东联合基金项目(U0633007)

PCR-RFLP analysis of large subunit rDNA of symbiotic dinoflagellates in scleractinian corals from Luhuitou fringing reef of Sanya, Hainan

Zhijun Dong¹^,²^,³, Hui Huang¹^,^*(), Liangmin Huang¹, Yuanchao Li¹, Xiubao Li¹

1 Key Laboratory of Marine Bio-resourses Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301
2 Graduate University of the Chinese Academy of Sciences, Beijing 100049
3 Hainan National Experiment Station of Tropical Marine Biology, Sanya 572000

Received:2008-04-02 Accepted:2008-05-21 Online:2008-09-20 Published:2008-09-20
Contact: Hui Huang

摘要/Abstract

摘要：

造礁石珊瑚共生藻的系统分类研究对于理解珊瑚礁生态系统对全球变化的响应具有十分重要的意义。本研究利用PCR技术扩增核糖体基因大亚基片段以及限制性片段长度多态性(RFLP)的方法, 对海南三亚鹿回头岸礁的8科14属22种造礁石珊瑚的共生藻组成进行了研究。结果表明鹿回头岸礁造礁石珊瑚共生藻以C系群为优势系群, 偶尔发现D系群与鹿角杯形珊瑚(Pocilolpora damicornis)和黄癣蜂巢珊瑚(Favia favus)共生; 另外丑鹿角珊瑚(Acropora horrida)和丛生盔形珊瑚(Galaxea fascicularis)可以同时与C系群和D系群共生。共生多型性的发现暗示珊瑚与共生藻的共生关系具有灵活性。研究结果同样显示共生藻的核糖体基因大片段的DNA多态性偏低。未来应该结合其他的分子标记对鹿回头岸礁造礁石珊瑚共生藻的DNA多态性进行更深入的研究。

关键词: 中国南海, 造礁石珊瑚, 共生藻, DNA多态性

Abstract

The taxonomy of symbiotic dinoflagellates in scleractinian corals is important in understanding how coral reefs will respond to global climate change. We investigated the composition of the dinoflagellate genus Symbiodinium in 22 species of scleractinian corals from 14 genera and eight families in Luhuitou fringing reef of Sanya, using polymerase chain reaction (PCR) of the large subunit rDNA and restriction fragment length polymorphism (RFLP). The results showed that the dinoflagellate genus Symbiodinium clade C was dominant in the study area. Pocillopora damicornis and Favia favusharbor Symbiodinium Clade D, while Galaxea fascicularis and Acropora horrida can harbor more than one type of symbiont (Symbiodinium Clade C and D). Polymorphic symbiosis suggested that the flexibility of the symbiosis between corals and Symbiodinium. The large subunit rDNA polymorphisms of symbiotic dinoflagellates was lower. Further study is needed to investigate zooxanthellae diversity using other molecular markers.

Key words: South China Sea, scleractinian corals, symbiotic dinoflagellates, DNA polymorphisms

董志军, 黄晖, 黄良民, 李元超, 李秀保 (2008) 运用PCR-RFLP方法研究三亚鹿回头岸礁造礁石珊瑚共生藻的组成. 生物多样性, 16, 498-502. DOI: 10.3724/SP.J.1003.2008.08074.

Zhijun Dong, Hui Huang, Liangmin Huang, Yuanchao Li, Xiubao Li (2008) PCR-RFLP analysis of large subunit rDNA of symbiotic dinoflagellates in scleractinian corals from Luhuitou fringing reef of Sanya, Hainan. Biodiversity Science, 16, 498-502. DOI: 10.3724/SP.J.1003.2008.08074.

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链接本文: https://www.biodiversity-science.net/CN/10.3724/SP.J.1003.2008.08074

https://www.biodiversity-science.net/CN/Y2008/V16/I5/498

图/表 2

图1 造礁石珊瑚共生藻28S rDNA基因5'端片段扩增(A,B,C)及RsaI酶切的RFLP分析(D,E,F)。M, DNA marker; 10: 鹿角杯形珊瑚; 01: Acropora cerealis; 07: 丑鹿角珊瑚; 15: 浪花鹿角珊瑚; 18-20, 26, 28-30, 32, 34: 多孔鹿角珊瑚; 14: Acropora sarmentosa; 06, 17: 十字牡丹珊瑚; 12: 皱纹厚丝珊瑚; 13: 标准厚丝珊瑚; 03, 21, 22, 27, 31, 33: 澄黄滨珊瑚; 08: 扁缩滨珊瑚; 04: Porites murrayensis; 02: 细角孔珊瑚; 38: 二异角孔珊瑚; 23-25: 丛生盔形珊瑚; 35: 腐蚀刺柄珊瑚; 41: 黄癣蜂巢珊瑚; 11: 梳状菊花珊瑚; 16: 锯齿刺星珊瑚; 39: 同双星珊瑚; 37: 中华扁脑珊瑚; 09: 泡囊珊瑚

Fig. 1 Electrophoresis of the PCR-amplified 5' end of the 28S rDNA of Symbiodinium3(A, B, C) and RFLP genotypes of Symbiodiniumobtained from different colonies of scleractinian corals restricted by RsaI (D, E, F). M, DNA marker; 10, Pocillopora damicornis; 01, Acropora cerealis; 07, Acropora horrida; 15, Acropora cytherea; 18-20, 26, 28-30, 32, and 34, Acropora millepora; 14, Acropora sarmentosa; 06 and 17, Pavona decussata; 12, Pachyseris rugosa; 13, Pachyseris speciosa; 03, 21, 22, 27, 31, and 33, Porites lutea; 08, Porites compressa; 04, Porites murrayensis; 02, Goniopora gracilis; 38, Goniopora duofasciata; 23-25, Galaxea fascicularis; 35, Hydnophora exesa; 41, Favia favus; 11, Goniastrea pectinata; 16, Cyphastrea serailia; 39, Diploastrea heliopora; 37, Platygyra sinensis; 09, Plerogyra sinuosa.

表1 鹿回头造礁石珊瑚种类及其共生藻系群

Table 1 Scleractinian coral species and their symbiotic dinoflagellates in Luhuitou fringing reef of Sanya

珊瑚种类 Coral species	采样数量 Sample size	共生藻系群 Symbiodinium clade
杯形珊瑚科 Pocilloporidae
杯形珊瑚属 Pocillopora
鹿角杯形珊瑚 P. damicornis	1	D
鹿角珊瑚科 Acroporidae
鹿角珊瑚属 Acropora
丑鹿角珊瑚 A. horrida	1	C & D
浪花鹿角珊瑚 A. cytherea	1	C
多孔鹿角珊瑚 A. millepora	9	C
A. cerealis*	1	C
A. sarmentosa*	1	C
菌珊瑚科 Agariciidae
牡丹珊瑚属 Pavona
十字牡丹珊瑚 P. decussata	2	C
厚丝珊瑚属 Pachyseris
皱纹厚丝珊瑚 P. rugosa	1	C
标准厚丝珊瑚 P. speciosa	1	C
滨珊瑚科 Poritidae
滨珊瑚属 Porites
澄黄滨珊瑚 P. lutea	6	C
扁缩滨珊瑚 P. compressa	1	C
P. murrayensis*	1	C
角孔珊瑚属 Goniopora
细角孔珊瑚 G. gracilis	1	C
二异角孔珊瑚 G. duofasciata	1	C
枇杷珊瑚科 Oculinidae
盔形珊瑚属 Galaxea
丛生盔形珊瑚 G. fascicularis	3	C & D
裸肋珊瑚科 Merulinidae
刺柄珊瑚属 Hydnophora
腐蚀刺柄珊瑚 H. exesa	1	C
蜂巢珊瑚科 Faviidae
蜂巢珊瑚属 Favia
黄癣蜂巢珊瑚 F. favus	1	D
菊花珊瑚属 Goniastrea
梳状菊花珊瑚 G. pectinata	1	C
刺星珊瑚属 Cyphastrea
锯齿刺星珊瑚 C. serailia	1	C
双星珊瑚属 Diploastrea
同双星珊瑚 D. heliopora	1	C
扁脑珊瑚属 Platygyra
中华扁脑珊瑚 P. sinensis	1	C
丁香珊瑚科 Caryophylliidae
泡囊珊瑚属 Plerogyra
泡囊珊瑚 P. sinuosa	1	C

参考文献 27

[1]	Baker AC (2001) Reef corals bleach to survive change. Nature, 411,765-766. DOI URL PMID
[2]	Baker AC (2003) Flexibility and specificity of coral-algal symbiosis: diversity, ecology, and biogeography of Symbiodinium. Annual Review of Ecology, Evolution, and Systematics, 34,661-689.
[3]	Baker AC, Starger CJ, McClanahan TR, Glynn PW (2004) Corals’ adaptive response to climate change. Nature, 430,741. URL PMID
[4]	Berkelmans R, van Oppen MJH(2006) The role of zooxanthellae in the thermal tolerance of corals: a ‘nugget of hope’ for coral reefs in an era of climate change. Proceedings of the Royal Society of London Series B: Biological Sciences, 273,2305-2312.
[5]	Carlos AA, Baillie BK, Kawachi M, Maruyama T (1999) Phylogenetic position of Symbiodinium (Dinophyceae) isolates from tridacnids (Bivalvia), cardiids (Bivalvia), a sponge (Porifera), a soft coral (Anthozoa), and a free living strain. Journal of Phycology, 35,1054-1062.
[6]	Chen CA, Lam KK, Nakano Y, Tsai WS (2003) A stable association of the stress-tolerant zooxanthellae, Symbiodinium clade D, with the low-temperature-tolerant coral, Oulastrea crispata (Scleractinia: Faviidae) in subtropical non-reefal coral communities. Zoological Studies, 42,540-550.
[7]	Chen CA, Wang JT, Fang LS, Yang YW (2005a) Fluctuating algal symbiont communities in Acropora palifera (Cnidaria; Scleractinia) from Taiwan. Marine Ecology Progress Series, 295,343-347.
[8]	Chen CA, Yang YW, Wei NV, Tsai WS, Fang LS (2005b) Symbiont diversity in scleractinian corals from tropical reefs and subtropical non-reef communities in Taiwan. Coral Reefs, 24,11-22.
[9]	Davies PS (1993) Endosymbiosis in marine cnidarians. In:,Plant-Animal Interactions in the Marine Benthos (eds John DM, Hawkins SJ, Price JH), pp. 511-540. Clarendon, Oxford.
[10]	Hoegh-Guldberg O (1999) Climate change, coral bleaching and the future of the world’s coral reefs. Marine Freshwater Research, 50,839-866.
[11]	Huang H, Dong ZJ, Huang LM, Zhang JB (2006) Restriction fragment length polymorphism analysis of large subunit rDNA of symbiotic dinoflagellates from scleractinian corals in the Zhubi coral reef of the Nansha Islands. Journal of Integrative Plant Biology, 48,148-152.
[12]	Iglesias-Prieto R, Beltrán VH, LaJeunesse TC, Reyes-Bonilla H, Thome PE (2004) Different algal symbionts explain the vertical distribution of dominant reef corals in the eastern Pacific. Proceedings of the Royal Society of London Series B: Biological Sciences, 271,1757-1763. DOI URL PMID
[13]	Kinzie RA III, Takayama M, Santos SR (2001) The adaptive bleaching hypothesis: experimental tests of critical assumptions. The Biological Bulletin, 200,51-58.
[14]	LaJeunesse TC, Trench RK (2000) Biogeography of two species of Symbiodinium (Freudenthal) inhabiting the intertidal sea anemone Anthopleura elegantissima (Brandt). The Biological Bulletin, 199,126-134. DOI URL PMID
[15]	Lewis CL, Coffroth MA (2004) The acquisition of exogenous algal symbionts by an octocoral after bleaching. Science, 304,1490-1492. URL PMID
[16]	Pochon X, Pawlowski J, Zaninetti L, Rowan R (2001) High genetic diversity and relative specificity among Symbiodinium-like endosymbiotic dinoflagellates in soritid foraminiferans. Marine Biology, 139,1069-1078.
[17]	Pochon X, Montoya-Burgos JI, Stadelmann B, Pawlowski J (2006) Molecular phylogeny, evolutionary rates, and divergence timing of the symbiotic dinoflagellate genus Symbiodinium. Molecular Phylogenetics and Evolution, 38,20-30. URL PMID
[18]	Rodriguez-Lanetty M (2003) Evolving lineages of Symbiodinium-like dinoflagellates based on ITS1 rDNA. Molecular Phylogenetics and Evolution, 28,152-168. DOI URL PMID
[19]	Rowan R (2004) Thermal adaptation in reef coral symbionts. Nature, 430,742.
[20]	Rowan R, Powers DA (1991) A molecular genetic classification of zooxanthellae and the evolution of animal-algal symbiosis. Science, 251,1348-1351.
[21]	Rowan R, Knowlton N, Baker AC, Jara J (1997) Landscape ecology of algal symbiont communities explains variation in episodes of coral bleaching. Nature, 388,265-269. DOI URL PMID
[22]	Toller WW, Rowan R, Knowlton N (2001a) Zooxanthellae of the Montastraea annularis species complex: patterns of distribution of four taxa of Symbiodinium on divergent reefs and across depths. The Biological Bulletin, 201,348-359.
[23]	Toller WW, Rowan R, Knowlton N (2001b) Repopulation of zooxanthellae in the Caribbean corals Montastraea annularis and M. faveolata following experimental and disease associated bleaching. The Biological Bulletin, 201,360-373. URL PMID
[24]	Yu DP (于登攀), Zou RL (邹仁林) (1996) Study on the species diversity of the scleractinian coral community on Luhuitou fringing reef. Acta Ecologica Sinica(生态学报), 16,469-475. (in Chinese with English abstract)
[25]	Zhang QM (张乔民), Shi Q (施祺), Chen G (陈刚), Fong TCW (方静威), Wong DCC (黄志俊), Huang H (黄晖), Wang HK (王汉奎), Zhao MX (赵美霞) (2006) Status monitoring and health assessment of Luhuitou fringing reef of Sanya, Hainan, China. Chinese Science Bulletin (科学通报), 51,81-88. (in Chinese)
[26]	Zou RL (邹仁林) (2001) Fauna Sinica: Hermatypic Coral(中国动物志‧造礁石珊瑚). Science Press,Beijing. (in Chinese)
[27]	Zou RL (邹仁林), Song SW (宋善文), Ma JH (马江虎) (1975) Shallow Water Hermatypic Coral in Hainan Island (海南岛浅水造礁石珊瑚). Science Press,Beijing. (in Chinese)

运用PCR-RFLP方法研究三亚鹿回头岸礁造礁石珊瑚共生藻的组成

PCR-RFLP analysis of large subunit rDNA of symbiotic dinoflagellates in scleractinian corals from Luhuitou fringing reef of Sanya, Hainan

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