运用PCR-RFLP方法研究三亚鹿回头岸礁造礁石珊瑚共生藻的组成

doi:10.3724/SP.J.1003.2008.08074

生物多样性 ›› 2008, Vol. 16 ›› Issue (5): 498-502. DOI: 10.3724/SP.J.1003.2008.08074

运用PCR-RFLP方法研究三亚鹿回头岸礁造礁石珊瑚共生藻的组成

董志军¹^,²^,³, 黄晖¹^,^*(), 黄良民¹, 李元超¹, 李秀保¹

1 中国科学院南海海洋研究所海洋生物资源可持续利用重点实验室, 广州 510301
2 中国科学院研究生院, 北京 100049
3 中国科学院海南热带海洋生物实验站, 三亚 572000

收稿日期:2008-04-02 接受日期:2008-05-21 出版日期:2008-09-20 发布日期:2008-09-20
通讯作者: 黄晖
作者简介:*(coral@scsio.ac.cn)
基金资助:
中科院台站基金支持项目(YTZJJ0502);国家海洋局908专项资助项目(908-ST-01-08-珊瑚礁调查);国家自然科学基金(30200039);国家自然科学基金(90211015);国家自然科学基金(40776085);国家自然科学基金(40576052);国家基金委–广东联合基金项目(U0633007)

PCR-RFLP analysis of large subunit rDNA of symbiotic dinoflagellates in scleractinian corals from Luhuitou fringing reef of Sanya, Hainan

Dong Zhijun¹^,²^,³, Huang Hui¹^,^*(), Huang Liangmin¹, Li Yuanchao¹, Li Xiubao¹

1 Key Laboratory of Marine Bio-resourses Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301
2 Graduate University of the Chinese Academy of Sciences, Beijing 100049
3 Hainan National Experiment Station of Tropical Marine Biology, Sanya 572000

Received:2008-04-02 Accepted:2008-05-21 Online:2008-09-20 Published:2008-09-20
Contact: Huang Hui

摘要/Abstract

摘要：

造礁石珊瑚共生藻的系统分类研究对于理解珊瑚礁生态系统对全球变化的响应具有十分重要的意义。本研究利用PCR技术扩增核糖体基因大亚基片段以及限制性片段长度多态性(RFLP)的方法, 对海南三亚鹿回头岸礁的8科14属22种造礁石珊瑚的共生藻组成进行了研究。结果表明鹿回头岸礁造礁石珊瑚共生藻以C系群为优势系群, 偶尔发现D系群与鹿角杯形珊瑚(Pocilolpora damicornis)和黄癣蜂巢珊瑚(Favia favus)共生; 另外丑鹿角珊瑚(Acropora horrida)和丛生盔形珊瑚(Galaxea fascicularis)可以同时与C系群和D系群共生。共生多型性的发现暗示珊瑚与共生藻的共生关系具有灵活性。研究结果同样显示共生藻的核糖体基因大片段的DNA多态性偏低。未来应该结合其他的分子标记对鹿回头岸礁造礁石珊瑚共生藻的DNA多态性进行更深入的研究。

关键词: 中国南海, 造礁石珊瑚, 共生藻, DNA多态性

Abstract

The taxonomy of symbiotic dinoflagellates in scleractinian corals is important in understanding how coral reefs will respond to global climate change. We investigated the composition of the dinoflagellate genus Symbiodinium in 22 species of scleractinian corals from 14 genera and eight families in Luhuitou fringing reef of Sanya, using polymerase chain reaction (PCR) of the large subunit rDNA and restriction fragment length polymorphism (RFLP). The results showed that the dinoflagellate genus Symbiodinium clade C was dominant in the study area. Pocillopora damicornis and Favia favusharbor Symbiodinium Clade D, while Galaxea fascicularis and Acropora horrida can harbor more than one type of symbiont (Symbiodinium Clade C and D). Polymorphic symbiosis suggested that the flexibility of the symbiosis between corals and Symbiodinium. The large subunit rDNA polymorphisms of symbiotic dinoflagellates was lower. Further study is needed to investigate zooxanthellae diversity using other molecular markers.

Key words: South China Sea, scleractinian corals, symbiotic dinoflagellates, DNA polymorphisms

董志军, 黄晖, 黄良民, 李元超, 李秀保 (2008) 运用PCR-RFLP方法研究三亚鹿回头岸礁造礁石珊瑚共生藻的组成. 生物多样性, 16, 498-502. DOI: 10.3724/SP.J.1003.2008.08074.

Dong Zhijun, Huang Hui, Huang Liangmin, Li Yuanchao, Li Xiubao (2008) PCR-RFLP analysis of large subunit rDNA of symbiotic dinoflagellates in scleractinian corals from Luhuitou fringing reef of Sanya, Hainan. Biodiversity Science, 16, 498-502. DOI: 10.3724/SP.J.1003.2008.08074.

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链接本文: https://www.biodiversity-science.net/CN/10.3724/SP.J.1003.2008.08074

https://www.biodiversity-science.net/CN/Y2008/V16/I5/498

图/表 2

图1 造礁石珊瑚共生藻28S rDNA基因5'端片段扩增(A,B,C)及RsaI酶切的RFLP分析(D,E,F)。M, DNA marker; 10: 鹿角杯形珊瑚; 01: Acropora cerealis; 07: 丑鹿角珊瑚; 15: 浪花鹿角珊瑚; 18-20, 26, 28-30, 32, 34: 多孔鹿角珊瑚; 14: Acropora sarmentosa; 06, 17: 十字牡丹珊瑚; 12: 皱纹厚丝珊瑚; 13: 标准厚丝珊瑚; 03, 21, 22, 27, 31, 33: 澄黄滨珊瑚; 08: 扁缩滨珊瑚; 04: Porites murrayensis; 02: 细角孔珊瑚; 38: 二异角孔珊瑚; 23-25: 丛生盔形珊瑚; 35: 腐蚀刺柄珊瑚; 41: 黄癣蜂巢珊瑚; 11: 梳状菊花珊瑚; 16: 锯齿刺星珊瑚; 39: 同双星珊瑚; 37: 中华扁脑珊瑚; 09: 泡囊珊瑚

Fig. 1 Electrophoresis of the PCR-amplified 5' end of the 28S rDNA of Symbiodinium3(A, B, C) and RFLP genotypes of Symbiodiniumobtained from different colonies of scleractinian corals restricted by RsaI (D, E, F). M, DNA marker; 10, Pocillopora damicornis; 01, Acropora cerealis; 07, Acropora horrida; 15, Acropora cytherea; 18-20, 26, 28-30, 32, and 34, Acropora millepora; 14, Acropora sarmentosa; 06 and 17, Pavona decussata; 12, Pachyseris rugosa; 13, Pachyseris speciosa; 03, 21, 22, 27, 31, and 33, Porites lutea; 08, Porites compressa; 04, Porites murrayensis; 02, Goniopora gracilis; 38, Goniopora duofasciata; 23-25, Galaxea fascicularis; 35, Hydnophora exesa; 41, Favia favus; 11, Goniastrea pectinata; 16, Cyphastrea serailia; 39, Diploastrea heliopora; 37, Platygyra sinensis; 09, Plerogyra sinuosa.

表1 鹿回头造礁石珊瑚种类及其共生藻系群

Table 1 Scleractinian coral species and their symbiotic dinoflagellates in Luhuitou fringing reef of Sanya

珊瑚种类 Coral species	采样数量 Sample size	共生藻系群 Symbiodinium clade
杯形珊瑚科 Pocilloporidae
杯形珊瑚属 Pocillopora
鹿角杯形珊瑚 P. damicornis	1	D
鹿角珊瑚科 Acroporidae
鹿角珊瑚属 Acropora
丑鹿角珊瑚 A. horrida	1	C & D
浪花鹿角珊瑚 A. cytherea	1	C
多孔鹿角珊瑚 A. millepora	9	C
A. cerealis*	1	C
A. sarmentosa*	1	C
菌珊瑚科 Agariciidae
牡丹珊瑚属 Pavona
十字牡丹珊瑚 P. decussata	2	C
厚丝珊瑚属 Pachyseris
皱纹厚丝珊瑚 P. rugosa	1	C
标准厚丝珊瑚 P. speciosa	1	C
滨珊瑚科 Poritidae
滨珊瑚属 Porites
澄黄滨珊瑚 P. lutea	6	C
扁缩滨珊瑚 P. compressa	1	C
P. murrayensis*	1	C
角孔珊瑚属 Goniopora
细角孔珊瑚 G. gracilis	1	C
二异角孔珊瑚 G. duofasciata	1	C
枇杷珊瑚科 Oculinidae
盔形珊瑚属 Galaxea
丛生盔形珊瑚 G. fascicularis	3	C & D
裸肋珊瑚科 Merulinidae
刺柄珊瑚属 Hydnophora
腐蚀刺柄珊瑚 H. exesa	1	C
蜂巢珊瑚科 Faviidae
蜂巢珊瑚属 Favia
黄癣蜂巢珊瑚 F. favus	1	D
菊花珊瑚属 Goniastrea
梳状菊花珊瑚 G. pectinata	1	C
刺星珊瑚属 Cyphastrea
锯齿刺星珊瑚 C. serailia	1	C
双星珊瑚属 Diploastrea
同双星珊瑚 D. heliopora	1	C
扁脑珊瑚属 Platygyra
中华扁脑珊瑚 P. sinensis	1	C
丁香珊瑚科 Caryophylliidae
泡囊珊瑚属 Plerogyra
泡囊珊瑚 P. sinuosa	1	C

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运用PCR-RFLP方法研究三亚鹿回头岸礁造礁石珊瑚共生藻的组成

PCR-RFLP analysis of large subunit rDNA of symbiotic dinoflagellates in scleractinian corals from Luhuitou fringing reef of Sanya, Hainan

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