东乡野生稻根际可培养细菌多样性及其植物促生活性分析

doi:10.3724/SP.J.1003.2011.09002

生物多样性 ›› 2011, Vol. 19 ›› Issue (4): 476-484. DOI: 10.3724/SP.J.1003.2011.09002

所属专题：土壤生物与土壤健康

东乡野生稻根际可培养细菌多样性及其植物促生活性分析

罗菲¹^,², 汪涯², 曾庆桂¹, 颜日明¹, 张志斌¹, 朱笃¹^,²^,^*()

1 江西师范大学生命科学学院/江西省亚热带植物资源保护与利用重点实验室, 南昌 330022
2 宜春学院江西省天然药物活性成分研究重点实验室, 江西宜春 336000

收稿日期:2011-01-12 接受日期:2011-06-09 出版日期:2011-07-20 发布日期:2011-07-29
通讯作者: 朱笃
作者简介:* E-mail: zhudu12@163.com
基金资助:
江西省科技攻关项目(2009ZDN10600)

Diversity and plant growth promoting activities of the cultivable rhizobacteria of Dongxiang wild rice (Oryza rufipogon)

Fei Luo¹^,², Ya Wang², Qinggui Zeng¹, Riming Yan¹, Zhibin Zhang¹, Du Zhu¹^,²^,^*()

1 Key Laboratory of Protection and Utilization of Subtropical Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang 330022
2 Key Laboratory for Research on Active Ingredients in Natural Medicine of Jiangxi Province, Yichun University, Yichun 336000

Received:2011-01-12 Accepted:2011-06-09 Online:2011-07-20 Published:2011-07-29
Contact: Du Zhu

摘要/Abstract

摘要：

为了解江西东乡野生稻(Oryza rufipogon)不同生育期根际可培养细菌群落多样性动态, 从中寻找新的具有植物促生活性的微生物资源, 分别于2009年5月、8月和11月采集了东乡野生稻秧苗期、抽穗期和成熟期根际土壤, 采用稀释平板法分离到118株根际细菌, 并检测了其植物促生活性。16S rRNA基因序列分析结合菌体形态观察表明: 分离获得的118株细菌属于5大类群11个属20个种, 其中29株(24.6%)属于变形菌门α亚群(α-Proteobacteria)、9株(7.6%)属于变形菌门β亚群(β-Proteobacteria)、37株(31.4%)属于变形菌门γ亚群(γ-Proteobacteria)、41株(34.7%)属于厚壁菌门(Firmicutes)、2株(1.7%)属于产水菌门(Aquificae)。芽孢杆菌属(Bacillus)、泛菌属(Pantoea)和鞘氨醇单胞菌属(Sphingomonas)的菌株数占优势。东乡野生稻根际可培养细菌多样性丰富, Shannon-Wiener指数为2.037-2.741, 且细菌类群随水稻的生育期不同而改变。分离菌株的植物促生活性检测表明: 东乡野生稻根际蕴含着丰富的固氮、溶磷、产IAA和分泌铁载体等生物活性细菌, 其中泛菌属为优势根际促生细菌。此外, 根际可培养细菌数量、促生细菌数量和植物促生活性等在东乡野生稻秧苗期开始升高, 抽穗期到达高峰, 成熟期下降。研究结果表明东乡野生稻根际土壤微生物多样性丰富, 是根际促生细菌的重要资源库。

关键词: Oryza rufipogon, 根际细菌, 可培养细菌, 群落多样性, 植物促生活性

Abstract

In order to understand the diversity of cultivable rhizobacteria associated with Dongxiang wild rice at different growth stages and to discover new species and strains of plant growth-promoting rhizobacteria (PGPR), we isolated 118 bacteria strains with different colony morphology using the dilution plate counting method and tested their plant growth promoting activities (PGPA) in May, August and November 2009. Phylogenetic analysis based on partial 16S rRNA sequences indicated that 118 strains clustered into 11 genera, and the dominant bacteria genera were Bacillus, Pantoea and Sphingomonas. The rhizobacteria displayed a high diversity with Shannon-Wiener indices of 2.037-2.741. PGPA analyses indicated that most strains displayed PGPR effects, including nitrogen-fixation, phosphorus-solubilization, IAA-production and siderophore-secretion, and that the dominant PGPR was Pantoea. Additionally, we found that the total amount and PGPA of cultivable rhizobacteria began to increase gradually at seedling stage of Dongxiang wild rice, and reached its peak at heading stage, then decreased at grain filling stage. Our results show that the diversity of bacterial communities in the rhizosphere soil of Dongxiang wild rice is abundant, and that the rich rhizobacteria may be a fascinating PGPR reservoir.

Key words: Oryza rufipogon, rhizosphere bacteria, cultivable bacteria, community diversity, plant growth- promoting activities

罗菲, 汪涯, 曾庆桂, 颜日明, 张志斌, 朱笃 (2011) 东乡野生稻根际可培养细菌多样性及其植物促生活性分析. 生物多样性, 19, 476-484. DOI: 10.3724/SP.J.1003.2011.09002.

Fei Luo, Ya Wang, Qinggui Zeng, Riming Yan, Zhibin Zhang, Du Zhu (2011) Diversity and plant growth promoting activities of the cultivable rhizobacteria of Dongxiang wild rice (Oryza rufipogon). Biodiversity Science, 19, 476-484. DOI: 10.3724/SP.J.1003.2011.09002.

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链接本文: https://www.biodiversity-science.net/CN/10.3724/SP.J.1003.2011.09002

https://www.biodiversity-science.net/CN/Y2011/V19/I4/476

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参考文献 41

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类群 Microbe groups	代表菌株 Representative strains	基因登录号 GenBank accession no.	最近缘物种(基因登录号) Closest relatives in NCBI (GenBank accession no.)	最大相似度 Max. identity (%)	生长阶段(单菌落数) Growing stage (Colony number)
变形菌门α亚群 α-Proteobacteria	S109	GU998805	Sphingomonas sp. (AB461702)	99	HS(21)^a; GF(2)
	S22	HQ647260	Rhodopseudomonas palustris (AB250613.1)	99	HS(1); GF(1)
	S13	HQ647259	Methylobacterium fujisawaense(AB558142)	100	RS(2); HS(1); GF(1)
变形菌门β亚群 β-Proteobacteria	T26	HQ443228	Uncultured Cupriavidus sp. (GQ417899)	98	GF(1)
	T24	HQ647268	C. basilensis (GU171379.1)	98	RS(2); HS(1); GF(2)
	TS20	GU998815	Burkholderia cepacia (FJ652618)	99	HS(2); GF(1)
变形菌门γ亚群 γ-Proteobacteria	TS4	GU998802	Pantoea dispersa (GQ246183)	99	RS(4)^a; HS(6)^a; GF(1)
	T2	HQ443233	P. agglomerans (HM130693)	100	RS(6)^a; HS(6)^a; GF(1)
	TS52	GU998808	Uncultured Pseudomonadales bacterium (DQ169126)	99	GF(1)
	TS57	GU998820	P. aeruginosa (FJ907193)	99	GF(1)
	P6	GU998813	P. poae (FJ937922)	99	RS(2); HS(3)
	F32	HQ647251	P. fluorescens (GU198119)	100	RS(1)
	TS51	GU998810	Stenotrophomonas maltophilia (GU358076)	99	RS(2); HS(1); GF(2)
产水菌门 Aquificae	TS54	HQ647283	Chryseobacterium indologenes (AY050493)	98	GF(2)
厚壁菌门 Firmicutes	S71	GU998803	Bacillus subtilis (FJ392727)	99	RS(6)^a; HS(5)^a; GF(4)^a
	T184	HQ647278	B. megaterium (EU594558)	99	RS(3); HS(2); GF(5)^a
	T47	GU998812	B. koreensis (AY667496)	100	GF(3)^a
	S59	HQ443223	B. aryabhattai (GU563348)	99	HS(1); GF(2)
	T111	HQ443236	B. cereus (HM752769)	98	RS(1); HS(1); GF(3)^a
	T172	HQ647277	B. pumilus (FJ763642)	99	RS(2); HS(1); GF(2)

类群 Microbe groups	代表菌株 Representative strains	基因登录号 GenBank accession no.	最近缘物种(基因登录号) Closest relatives in NCBI (GenBank accession no.)	最大相似度 Max. identity (%)	生长阶段(单菌落数) Growing stage (Colony number)
变形菌门α亚群 α-Proteobacteria	S109	GU998805	Sphingomonas sp. (AB461702)	99	HS(21)^a; GF(2)
	S22	HQ647260	Rhodopseudomonas palustris (AB250613.1)	99	HS(1); GF(1)
	S13	HQ647259	Methylobacterium fujisawaense(AB558142)	100	RS(2); HS(1); GF(1)
变形菌门β亚群 β-Proteobacteria	T26	HQ443228	Uncultured Cupriavidus sp. (GQ417899)	98	GF(1)
	T24	HQ647268	C. basilensis (GU171379.1)	98	RS(2); HS(1); GF(2)
	TS20	GU998815	Burkholderia cepacia (FJ652618)	99	HS(2); GF(1)
变形菌门γ亚群 γ-Proteobacteria	TS4	GU998802	Pantoea dispersa (GQ246183)	99	RS(4)^a; HS(6)^a; GF(1)
	T2	HQ443233	P. agglomerans (HM130693)	100	RS(6)^a; HS(6)^a; GF(1)
	TS52	GU998808	Uncultured Pseudomonadales bacterium (DQ169126)	99	GF(1)
	TS57	GU998820	P. aeruginosa (FJ907193)	99	GF(1)
	P6	GU998813	P. poae (FJ937922)	99	RS(2); HS(3)
	F32	HQ647251	P. fluorescens (GU198119)	100	RS(1)
	TS51	GU998810	Stenotrophomonas maltophilia (GU358076)	99	RS(2); HS(1); GF(2)
产水菌门 Aquificae	TS54	HQ647283	Chryseobacterium indologenes (AY050493)	98	GF(2)
厚壁菌门 Firmicutes	S71	GU998803	Bacillus subtilis (FJ392727)	99	RS(6)^a; HS(5)^a; GF(4)^a
	T184	HQ647278	B. megaterium (EU594558)	99	RS(3); HS(2); GF(5)^a
	T47	GU998812	B. koreensis (AY667496)	100	GF(3)^a
	S59	HQ443223	B. aryabhattai (GU563348)	99	HS(1); GF(2)
	T111	HQ443236	B. cereus (HM752769)	98	RS(1); HS(1); GF(3)^a
	T172	HQ647277	B. pumilus (FJ763642)	99	RS(2); HS(1); GF(2)

生长阶段 Growth stages	多样性指数 H	均匀度指数 J	优势度指数 D	丰富度指数 S
秧苗期 RS	2.232	0.931	0.097	11
抽穗期 HS	2.037	0.772	0.208	14
成熟期 GF	2.741	0.948	0.074	18

生长阶段 Growth stages	多样性指数 H	均匀度指数 J	优势度指数 D	丰富度指数 S
秧苗期 RS	2.232	0.931	0.097	11
抽穗期 HS	2.037	0.772	0.208	14
成熟期 GF	2.741	0.948	0.074	18

东乡野生稻根际可培养细菌多样性及其植物促生活性分析

Diversity and plant growth promoting activities of the cultivable rhizobacteria of Dongxiang wild rice (Oryza rufipogon)

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