Biodiversity Science ›› 2013, Vol. 21 ›› Issue (6): 715-722.doi: 10.3724/SP.J.1003.2013.08152

• Orginal Article • Previous Article     Next Article

Diversity of calcium speciation in leaves of Primulina species (Gesneriaceae)

Qingwen Qi1, 2, Zhuan Hao1, 2, Junjie Tao1, 2, Ming Kang1, *()   

  1. 1 Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650
    2 University of the Chinese Academy of Sciences, Beijing 100049
  • Received:2013-07-03 Accepted:2013-10-28 Online:2013-12-02
  • Kang Ming E-mail:mingkang@scbg.ac.cn

Primulina is a genus containing typical “stone plants” or “cave plants” that show a high degree of edaphic specialization in the karst limestone regions of southwest China. Most species of the genus occur only on calcareous soils developed from carbonate bedrock, while a few species are found only on the red soil developed from the Danxia landform or acidic soil developed from sandshale bedrock. The aim of this study is to investigate the diversity and characteristics of calcium absorption and storage in Primulina from different soil substrates. Calcium in leaves was determined for plants sampled from 15 populations representing 11 Primulina species occurring on calcareous soil, red soil or acid soil. We analyzed the main types of calcium found in leaves, and compared the calcium content within and among species from different soil types. The results revealed a general high level of leaf calcium content in Primulina species compared with other plants from the karst regions of southwest China. However, we found a significant difference in calcium content among Primulina species from different soil types, with high average calcium content (2,285.6 mg/kg) in Primulina from calcareous soil relative to low levels present in Primulina from both acid soil (1,379.3 mg/kg) and Danxia red soil (1,329.1 mg/kg). The main form of calcium stored in most Primulina species (9 out of 11) was pectate calcium, which accounted for 31.6-64.2% of the total calcium in the leaves. In contrast, for two species, P. linearifolia and P. medica, which grow on soil with a pH > 8, the main calcium form was soluble calcium, which accounted for about 40% of the total calcium in plant leaves. In addition, differences in calcium amount and type were recorded within species from either the same or different soil types. These results suggest that there is variation in calcium speciation found in Primulina at both interspecific and intraspecific levels. Our findings provide a valuable basis for further studies on adaptive mechanisms of edaphic specialization in Primulina.

Key words: Primulina, karst plant, calcareous soil, acidic soil, calcium speciation, diversity

Table 1

Sampling information of the 15 Primulina populations in this study"

土壤基岩及编号
Bedrocks and code
种名
Species
采集地点
Sampling locations
土壤 pH
Soil pH
石灰岩 Limestone
ZJHZ01 西子报春苣苔 Primulina xiziae 浙江杭州 Hangzhou, Zhejiang -
DX03 牛耳朵 P. eburnean 湖南道县 Daoxian, Hunan 7.5
HNBT03 烟叶报春苣苔 P. heterotricha 海南保亭县 Baoting, Hainan 7.2
YLY01 线叶报春苣苔 P. linearifolia 广西武鸣县 Wuming, Guangxi 8.1
GXQT01 药用报春苣苔 P. medica 广西平乐县 Pingle, Guangxi 8.0
丹霞地貌 Danxia
GXRX01 钟冠报春苣苔 P. swinglei 广西容县 Rongxian, Guangxi 7.8
GDLC07 报春苣苔属一新种 Primulina sp. 广东乐昌 Lechang, Guangdong 7.7
DXS05 蚂蝗七 P. fimbrisepala 广东丹霞山 Danxia, Guangdong 7.6
GXZY01 蚂蝗七 P. fimbrisepala 广西资源县 Ziyuan, Guangxi 5.0
砂页岩 Sandshale
GDSZ01 中华报春苣苔 P. sinensis 广东深圳 Shenzhen, Guangdong 4.3
HNBT01 烟叶报春苣苔 P. heterotricha 海南保亭县 Baoting, Hainan 5.5
WG02 蚂蝗七 P. fimbrisepala 湖南武冈市 Wugang, Hunan 3.6
CBL01 蚂蝗七 P. fimbrisepala 广东始兴县 Shixing, Guangdong 3.8
LBJX01 齿萼报春苣苔 P. verecunda 广西金秀县 Jinxiu, Guangxi 3.3
GZJK01 钝齿报春苣苔 P. obtusidentata 贵州江口县 Jiangkou, Guizhou 4.2

Table 2

Extraction procedure and chemical fraction of calcium speciation in leaves"

提取顺序
Extraction order
提取剂
Extraction solution
主要钙形态
Calcium speciation
简写
Abbreviation
1 80% C2H5OH 硝酸钙与氯化钙 Calcium nitrate and calcium chloride AIC-Ca
2 Distilled water 水溶性有机酸钙 Water soluble calcium H2O-Ca
3 1 M NaCl 果胶酸钙 Calcium pectate NaCl-Ca
4 2% CH3COOH 磷酸钙与碳酸钙 Calcium phosphate and calcium carbonate HAC-Ca
5 0.6% HCl 草酸钙 Calcium oxalate HCl-Ca
残渣 Residual - 残渣钙(硅酸钙) Silicate calcium Res-Ca

Table 3

Average contents of calcium speciation and total calcium content (mg/kg) in leaves of Primulina from different soil types. The values in the parentheses represent the standard errors."

土壤基岩
Bedrock
果胶酸钙 NaCl-Ca 水溶性钙
H2O-Ca
硝酸钙和氯化钙 AIC-Ca 磷酸钙和碳酸钙
HAC-Ca
草酸钙
HCl-Ca
硅酸钙
Res-Ca
总钙
Total calcium
石灰岩 Limestone 959.4(90.1)A 710.6(197.9)A 437.8(80.9)A 82.7(12.1) 23.5(4.3)A 71.5(13.8)A 2,285.6(292.8)A
丹霞地貌 Danxia 733.0(95.3)B 296.5(58.0)B 137.1(28.1)C 83.2(22.7) 20.0(3.2)AB 59.2(10.4)B 1,329.1(133.2)B
砂页岩 Sandshale 691.5(54.5)B 287.0(53.4)B 241.3(32.4)B 83.0(10.2) 17.8(2.5)B 58.7(6.0)B 1,379.3(112.1)B

Table 4

Content of various calcium speciation (mg/kg) in leaves of the 15 Primulina populations. The values in the parentheses represent the standard errors."

编号
Code
果胶酸钙
NaCl-Ca
水溶性钙
H2O-Ca
硝酸钙和氯化钙
AIC-Ca
磷酸钙和碳酸钙
HAC-Ca
草酸钙
HCl-Ca
硅酸钙
Res-Ca
ZJHZ01 1,148.7(78.0)A 617.5(6.5)C 564.6(44.9)B 62.7(3.7)EF 19.8(0.7)EF 128.3(1.4)A
DX03 1,132.5(31.0)A 354.4(9.1)F 128.3(2.7)F 72.7(3.1)ED 40.5(1.6)A 35.1(1.3)G
HNBT03 656.1(25.1)D 243.7(6.6)H 530.8(5.4)B 69.0(0.5)ED 13.6(1.0)HJ 57.5(3.4)E
YLY01 782.8(10.7)C 847.9(16.0)B 342.8(9.3)C 73.0(1.2)ED 26.9(1.6)CB 65.0(1.6)ED
GXQT01 1,076.9 (16.0)A 1,489.6(53.9)A 622.7(41.7)A 136.3(5.4)B 16.7(0.9)HFG 71.6(1.3)D
GXRX01 427.0(14.1)F 160.8(9.3)I 224.4(6.4)D 39.0(2.5)G 10.1(0.3)J 72.8(0.7)CD
GDLC07 748.5(8.8)C 366.6(12.6)EF 68.3(2.8)G 158.2(8.9)A 27.2(1.4)CB 45.2(7.8)F
DXS05 811.0(6.3)C 210.4(5.2)IH 136.1(4.9)F 78.7(2.3)D 24.0(1.5)CD 33.4(1.0)G
GXZY01 945.7(15.2)B 448.3(11.8)D 119.6(1.9)G 57.0(1.7)F 18.8(1.1)EFG 85.4(0.8)B
GDSZ01 746.2(8.4)C 369.1(6.3)EF 351.7(3.7)C 126.7(2.7)B 9.1(0.3)J 48.6(2.1)F
HNBT01 514.8(10.7)E 298.1(5.8)G 206.7(5.0)ED 40.8(0.5)G 15.4(0.5)HG 68.1(2.0)D
WG02 902.9(7.0)B 416.2(9.5)ED 144.2(1.5)F 99.4(1.4)C 21.9(0.5)ED 71.2(1.7)D
CBL01 644.2(4.4)D 181.9(1.4)I 162.1(1.8)EF 69.1(0.4)ED 10.7(0.3)JI 35.6(0.5)G
LBJX01 816.5(2.2)C 425.8(2.1)D 249.1(0.7)D 90.5(1.3)C 22.3(0.8)ED 47.7(0.5)F
GZJK01 524.5(3.7)E 30.6(0.3)J 334.0(4.1)C 71.2(0.5)ED 27.8(0.5)B 81.0(1.3)CB

Fig. 1

Percentage of various calcium speciation of leaves for the 15 Primulina populations. Population codes and abbreviations of calcium speciation as shown in Table 1 and Table 2, respectively."

Table 5

Comparison of calcium speciation concentration in leaves of Primulina fimbrisepala and Primulina heterotricha from different bedrocks. The values in the parentheses represent standard errors."

物种
Species
土壤基岩
Bedrock
编号
Code
果胶酸钙
NaCl-Ca
水溶性钙H2O-Ca 硝酸钙
和氯化钙
AIC-Ca
磷酸钙
和碳酸钙
HAC-Ca
草酸钙
HCl-Ca
硅酸钙
Res-Ca
总钙
Total calcium
蚂蝗七
Primulina
fimbrisepala
丹霞地貌
Danxia
DXS05/
GXZY01
878.3(25.2) 329.4(42.6) 127.9(3.9)B 67.9(4.1)B 21.4(1.3) 59.4(26.0) 1,484.2(67.9)
砂页岩
Sandshale
WG02/
CBL01
773.5(45.9) 299.0(41.7) 153.2(3.4)A 84.3(5.4)A 16.3(2.0) 53.4(6.4) 1,379.7(97.8)
烟叶报春苣苔
Primulina
heterotricha
石灰岩
Limestone
HNBT03 656.1(25.1)A 243.7(6.6)B 530.8(5.4)A 69.0(0.5)A 13.6(1.0) 57.5(3.4) 1,570.6(21.0)
砂页岩
Sandshale
HNBT01 514.8(10.7)B 298.1(5.8)A 206.7(5.0)B 40.8(0.5)B 15.4(0.5) 68.1(2.0) 1,144.0(6.7)
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