Biodiversity Science ›› 2009, Vol. 17 ›› Issue (5): 490-498.doi: 10.3724/SP.J.1003.2009.09101

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Diversity and phylogenetic analysis of planktonic bacteria in eutrophic zone of Lake Wuliangsuhai

Xinxin Sun, Huirong Liu, Fuying Feng*, Jianyu Meng, Heng Li, Malina   

  1. Institute for Applied & Environmental Microbiology of Bioengineering College, Inner Mongolia Agriculture University, Huhhot 010018
  • Received:2009-04-16 Online:2009-09-20
  • Fuying Feng

The relationship between the bacterial communities and eutrophication of aquatic ecosystem is not clear. We investigated and analyzed the planktonic bacterial diversity and phylogeny of Wuliangsuhai Lake by using the construction of 16S rRNA gene clone library, and discussed the relationship between the bacterial community and eutrophication. Totally 87 clones were digested with Hae III and were grouped into 23 operational taxonomic units (OTUs). The estimate of library coverage showed that the library coverage value was of 73.6%, which indicated that the clone library could provide a fine inventory of bacterial diversity in the lake. Each representative clone of the 23 types was finally sequenced and homology analysis showed that the majority were affiliated to α-, β-, γ-Proteobacteria, Bacteroidetes, and Actinobacteria, which accounted for 10.3%, 41.4%, 4.6% and 6.9%, respectively. However, unlike bacterial composition of typical freshwater, the water had 10.3% of total bacteria slightly halo-and-alkaline-philic, making the composition unique. Moreover, 83.9% of total bacteria were lower than 97% of homology with cultured species, of which 58.9% were even failed to be identified at genus level, while ones (16.1% of total bacteria) affiliated to the cultured with the ability for pollutants degradation. Bacteroidetes and Firmicutes, and some groups of β-Proteobacteria came into dominant groups in the water, which could be the result of responding to the eu-trophication.

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[4] BU Ren-Cang, CHANG Yu, HU Yuan-Man, LI Xiu-Zhen, HE Hong-Shi. SENSITIVITY OF CONIFEROUS TREES TO ENVIRONMENTAL FACTORS AT DIFFERENT SCALES IN THE SMALL XING’AN MOUNTAINS, CHINA[J]. Chin J Plan Ecolo, 2008, 32(1): 80 -87 .
[5] Ramanjulu Sunkar and Jian-Kang Zhu. Micro RNAs and Short-interfering RNAs in Plants[J]. J Integr Plant Biol, 2007, 49(6): 817 -826 .
[6] . [J]. Chin Bull Bot, 1994, 11(专辑): 13 .
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