Biodiv Sci ›› 2016, Vol. 24 ›› Issue (11): 1296-1305.  DOI: 10.17520/biods.2016202

Special Issue: 昆虫多样性与生态功能

• Original Papers: Animal Diversity • Previous Articles     Next Articles

Estimation of species richness of moths (Insecta: Lepidoptera) based on DNA barcoding in Suqian, China

Qian Jin1,2, Fen Chen1, Guijie Luo1, Weijia Cai1, Xu Liu1, Hao Wang1, Caiqing Yang2, Mengdi Hao2, Aibing Zhang2,*()   

  1. 1 Suqian Institute of Agricultural Sciences, Jiangsu Academy of Agricultural Sciences, Suqian, Jiangsu 223800
    2 College of Life Sciences, Capital Normal University, Beijing 100048
  • Received:2016-07-22 Accepted:2016-11-02 Online:2016-11-20 Published:2016-12-14
  • Contact: Zhang Aibing


To explore the feasibility of assessing species diversity using DNA barcoding, we investigated this approach by focusing on moths species (Lepidoptera) in Suqian, China. The study evaluated community species richness and rank-abundance curves using the DNA barcoding method, and compared it with the traditional morphology method. Results indicated that there was no significant difference between the DNA barcode-based approach and the morphology-based approach. All DNA barcode-based rank-abundance curves gave similar and clear patterns when compared with morphology-based curves (Kolmogorov-Smirnov two sample test, P > 0.05). Our results indicate that the DNA barcode-based approach is able to be used to estimate species richness.

Key words: Lepidoptera, DNA barcoding, species richness, rank-abundance curves