Allozyme genetic variation at six loci (Per-2, Got-2, Est-2, Gpi-2, Idh-1, Idh-2 ) in eight populations of C. mollissima was invested using starch gel electrophoresis. The result showed very high genetic variation in the populations with the proportion of polymorphic loci of 97.9% in the average expected heterozygosity of 0.452. In general, about 89.2% of the genetic variation was attributed within populations, while the remaining 10.8% among populations. The genetic distance ranged from 0.036～0.394. There existed obvious difference, especially in a few locus .The primary result indicted the high level of genetic variation and difference could be closely related to the long gene migration flow which attributed to the diversity of its habitat, the outcrossing-winded character and so on.