Genetic diversity and population structure of Saurogobio dabryi under cascade water conservancy projects in the Jialing River

doi:10.17520/biods.2023160

Abstract

Abstract:

Aims: In recent years, with nearing the completion of channelization project of the Jialing River, the fragmentation of aquatic habitats, the decline of fish resources and other issues caused by the dams are becoming increasingly acute. Under the circumstances, it has been a worrying problem that whether the fish genetic diversity has been affected. The aim of this study is to analyze the genetic diversity and genetic structure of Saurogobio dabryi population under the background of cascade water conservancy project in the Jialing River by using mitochondrial control region sequence and microsatellite loci as molecular markers.

Methods: From July to December in 2018, 113 tails of S. dabryi were collected from Jialing River where the sampled sites were Chaotian District (CT), Cangxi County (CX), Peng’an County (PA) and Hechuan District (HC), respectively. The genetic diversity and genetic structure of S. dabryi population under the background of cascade water conservancy project were studied by using the mtDNA control region sequences and 10 microsatellite loci.

Results: The results showed that in genetic diversity analysis, whether mitochondria data or microsatellite marker, the genetic diversity parameters of HC population (PIC = 0.543, π = 0.0264, Hd = 0.940) were significantly higher than upstream and midstream (CT, CX and PA) populations. It was speculated that this phenomenon may be associated with HC population was located in the Yangtze river mainstream intersection. On the contrary, the genetic diversity and haploid number of CT population was higher, which may be associated with the population expansion in CT population, recently. In the analysis of population structure, CT, CX and PA populations were in the same branch and HC population in an independent branch. Haplotype network diagram and phylogenetic analysis showed the same result, which might be related to the geographical isolation caused by water conservancy projects and the formation of downward gene flow caused by water release.

Conclusion: The research results not only reveal the genetic diversity characteristics, population structure and population dynamics of S. dabryi population in each section of Jialing River under the background of cascade water conservancy project development, but also provide basic data for the genetic research of small and medium-sized fish in Jialing River in the future. By comparing the results of this study with the morphological differences, it was verified that there were also significant genetic differences in the population of S. dabryi in each section of Jialing River under the condition of significant differences in external morphology, so the phenomenon indicates that the natural geographical differences and genetic differences shaped the genetic pattern of species together.

Key words: cascade water conservancy projects, Jialing River, Saurogobio dabryi, genetic diversity

Yiyue He, Yuying Liu, Fubin Zhang, Qiang Qin, Yu Zeng, Zhenyu Lü, Kun Yang. Genetic diversity and population structure of Saurogobio dabryi under cascade water conservancy projects in the Jialing River[J]. Biodiv Sci, 2023, 31(11): 23160.

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URL: https://www.biodiversity-science.net/EN/10.17520/biods.2023160

https://www.biodiversity-science.net/EN/Y2023/V31/I11/23160

Figures/Tables 11

References 42

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种群 Populations	样本数(尾) Sample size	体长 Body length (mm)	湿重 Wet weight (g)	纬度 Latitude	经度 Longitude
朝天区 CT	27	100.12 ± 26.81	15.71 ± 10.27	32.65° N	105.89° E
苍溪县 CX	28	132.04 ± 11.15	27.75 ± 6.47	31.74° N	105.96° E
蓬安县 PA	30	109.95 ± 16.58	25.43 ± 33.82	31.04° N	106.40° E
合川区 HC	28	114.84 ± 11.07	17.42 ± 4.40	29.98° N	106.29° E

种群 Populations	样本数(尾) Sample size	体长 Body length (mm)	湿重 Wet weight (g)	纬度 Latitude	经度 Longitude
朝天区 CT	27	100.12 ± 26.81	15.71 ± 10.27	32.65° N	105.89° E
苍溪县 CX	28	132.04 ± 11.15	27.75 ± 6.47	31.74° N	105.96° E
蓬安县 PA	30	109.95 ± 16.58	25.43 ± 33.82	31.04° N	106.40° E
合川区 HC	28	114.84 ± 11.07	17.42 ± 4.40	29.98° N	106.29° E

位点 Loci	引物序列 Primer sequence (5'-3')	重复单元 Repeat motif	片段大小 Size range (bp)	退火温度 T_m (℃)	参考来源 Reference source	登录号 Accession number
SD01	GCTCCATTCCCAACCTATAACACAT GCCTTGATGAACGGTTATGTATG	(ACAT)₁₀	98	61	sdF5010	KX250317
SD02	GTGGCTCCTTCCCTTTCACAGAGCA GATTGTACATGTGGCTCAAG	(GCA)₉	116	62	sdF6400	KX25347
SD03	ATGACAAAGGAAAACCACGAGAAA TGCAGATACAGCGCATCACTTTAG	(AAAC)₁₆	132	62	sdF5261	KX250326
SD04	CACAGACGAGGATTCCAGTCGACA TGCAGTCCCTTACCTGTTCTC	(GAG)₅	154	65	sdF6273	KX250343
SD05	TCCGTTGTTTAGGCTACTGATCAAA TGAGATGACATGACGATAGCTGTG	(GATA)₁₇	155	63	sdF6479	KX250350
SD07	ACACTACTCGTCTGCCGCAAACAA TGCAGATTGTTTCAAAGCAG	(AAAT)₂	111	53	sdF7209	KX250353
SD08	AACTGTAGGGCACGACAAATTGAT AGTCTAAACCCGTCTGCAAGAATG	(AGAC)₁₈	232	56	sdF5145	KX250320
SD09	TCTCAGATGACGTTGAGCATATTGA CATTCATCTGGGCTCACTAAAACA	(ATCT)₁₅	151	57	sdF5163	KX250321
SD10	TTTCTGTACTTGTTAGTTTGGGGTC AGATCAATTAAATGCATCATTGCTGA	(TTTC)₆	156	56	sdF5351	KX250333
SD11	CGTCTAGTGCTGAAGGAGGTGAGT TCTCAGCCTGGAACACAGAGAGAT	(ACTC)₂₅	123	62	sdF6476	KX25329

位点 Loci	引物序列 Primer sequence (5'-3')	重复单元 Repeat motif	片段大小 Size range (bp)	退火温度 T_m (℃)	参考来源 Reference source	登录号 Accession number
SD01	GCTCCATTCCCAACCTATAACACAT GCCTTGATGAACGGTTATGTATG	(ACAT)₁₀	98	61	sdF5010	KX250317
SD02	GTGGCTCCTTCCCTTTCACAGAGCA GATTGTACATGTGGCTCAAG	(GCA)₉	116	62	sdF6400	KX25347
SD03	ATGACAAAGGAAAACCACGAGAAA TGCAGATACAGCGCATCACTTTAG	(AAAC)₁₆	132	62	sdF5261	KX250326
SD04	CACAGACGAGGATTCCAGTCGACA TGCAGTCCCTTACCTGTTCTC	(GAG)₅	154	65	sdF6273	KX250343
SD05	TCCGTTGTTTAGGCTACTGATCAAA TGAGATGACATGACGATAGCTGTG	(GATA)₁₇	155	63	sdF6479	KX250350
SD07	ACACTACTCGTCTGCCGCAAACAA TGCAGATTGTTTCAAAGCAG	(AAAT)₂	111	53	sdF7209	KX250353
SD08	AACTGTAGGGCACGACAAATTGAT AGTCTAAACCCGTCTGCAAGAATG	(AGAC)₁₈	232	56	sdF5145	KX250320
SD09	TCTCAGATGACGTTGAGCATATTGA CATTCATCTGGGCTCACTAAAACA	(ATCT)₁₅	151	57	sdF5163	KX250321
SD10	TTTCTGTACTTGTTAGTTTGGGGTC AGATCAATTAAATGCATCATTGCTGA	(TTTC)₆	156	56	sdF5351	KX250333
SD11	CGTCTAGTGCTGAAGGAGGTGAGT TCTCAGCCTGGAACACAGAGAGAT	(ACTC)₂₅	123	62	sdF6476	KX25329

种群 Populations	mtDNA线粒体控制区 mtDNA control region						微卫星位点 Microsatellite loci
种群 Populations	N	H	Hd	π	n	k	A	Ai	Ho	He	PIC	Fis
朝天区 CT	27	18	0.929	0.0180	94	16.328	4.500	4.500	0.493	0.433	0.391	-0.139
苍溪县 CX	28	10	0.877	0.0100	43	9.050	4.600	5.711	0.273	0.378	0.302	0.152
蓬安县 PA	30	14	0.875	0.0120	89	10.863	6.400	6.169	0.357	0.490	0.443	0.275
合川区 HC	28	17	0.940	0.0264	129	23.818	8.700	8.319	0.483	0.579	0.543	0.150
总计 Total	113	39	0.894	0.0348	203	12.654	12	5.923	0.302	0.438	0.531	0.145