Diversity of Cytospora chrysosperma from different hosts in Xinjiang

doi:10.17520/biods.2019180

Abstract

Abstract:

Cytospora chrysosperma is a very important pathogenic fungus that can be infects many different trees. Currently, the diversity, phylogenetic and genetic relationships of C. chrysosperma remain unclear as host differences could cause substantial genetic and diverse differences among C. chrysosperma strains. To analyze the genetic, phylogenetic, and current diversity of C. chrysosperma, physiological and biochemical characteristics and genetic tests were performed from different hosts in Xinjiang. Using molecular markings Biolog-FF and ISSR, 47 strains of C. chrysosperma have been divided into 15 types. Strains were cultured and displayed varying culture characteristics and carbon source utilization and metabolic capacity traits. Each strain tended to utilize more carbon over time, however, strains varied in their carbon source preferences and metabolic capacities. Using cluster analysis, 47 strains of C. chrysosperma divided into two clusters based on genetic similarity (0.58), suggesting genetic structure is essential to understanding C. chrysosperma diversity, as host species and geographical origins were not significant.

Key words: Cytospora chrysosperma, cultural characteristics, Biolog-FF, ISSR

Ying Zhao, Rong Ma, Yongxiang Yin, Zhidong Zhang, Chengming Tian. Diversity of Cytospora chrysosperma from different hosts in Xinjiang[J]. Biodiv Sci, 2019, 27(10): 1122-1131.

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URL: https://www.biodiversity-science.net/EN/10.17520/biods.2019180

https://www.biodiversity-science.net/EN/Y2019/V27/I10/1122

Figures/Tables 8

References 25

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菌株 Isolate	寄主 Host	采集时间 Collecting time	采集地点 Location
319-1	苹果 Malus sp.	2011/8/7	昌吉吉木萨尔县 Jimusar County, Changji Prefecture
790-2	苹果 Malus sp.	2015/6/28	塔城地区塔城市 Tacheng, Tacheng Prefecture
908	苹果 Malus sp.	2015/7/17	塔城地区乌苏市 Wusu, Tacheng Prefecture
936	苹果 Malus sp.	2015/8/4	塔城地区塔城市 Tacheng, Tacheng Prefecture
701	榆树 Ulmus sp.	2015/4/18	吐鲁番市鄯善县 Shanshan County, Turpan City
1155	榆树 Ulmus sp.	2015/8/20	昌吉州奇台县 Qitai County, Changji Prefecture
600-2	胡杨 Populus euphratica	2012/8/20	喀什地区泽普县 Zepu County, Kashgar Prefecture
626	新疆杨 Populus alba	2012/8/20	喀什地区喀什市 Kashgar City, Kashgar Prefecture
686-1	山杨 Populus davidiana	2014/8/31	塔城地区塔城市 Tacheng City, Tacheng Prefecture
686-2	山杨 Populus davidiana	2014/9/1	塔城地区塔城市 Tacheng City, Tacheng Prefecture
702	小叶杨 Populus simonii	2015/4/18	吐鲁番市鄯善县 Shanshan County, Turpan City
737	钻天杨 Populus nigra	2015/6/4	哈密地区哈密市 Hami City, Hami Prefecture
739	钻天杨 Populus nigra	2015/6/4	哈密地区哈密市 Hami City, Hami Prefecture
740	钻天杨 Populus nigra	2015/6/4	哈密地区哈密市 Hami City, Hami Prefecture
742	钻天杨 Populus nigra	2015/6/4	哈密地区哈密市 Hami City, Hami Prefecture
793	俄罗斯杨 Populus russkii	2015/6/29	塔城地区塔城市 Tacheng City, Tacheng Prefecture
882	北京杨 Populus beijingensis	2015/7/22	塔城地区乌苏市 Wusu City, Tacheng Prefecture
891-1	俄罗斯杨 Populus russkii	2015/7/17	塔城地区乌苏市 Wusu City, Tacheng Prefecture
891-3	俄罗斯杨 Populus russkii	2015/7/17	塔城地区乌苏市 Wusu City, Tacheng Prefecture
904	大叶杨 Populus lasiocarpa	2015/7/16	塔城地区乌苏市 Wusu City, Tacheng Prefecture
914-1	杨树 Populus sp.	2015/7/21	塔城地区乌苏市 Wusu City, Tacheng Prefecture
934	新疆杨 Populus alba	2015/8/3	塔城地区乌苏市 Wusu City, Tacheng Prefecture
1074-2	胡杨 Populus euphratica	2015/8/26	吐鲁番市托克逊县 Toxon County, Turpan City
1076	胡杨 Populus euphratica	2015/8/26	吐鲁番市托克逊县 Toxon County, Turpan City
812-1	柳树 Salix sp.	2015/7/1	塔城地区塔城市 Tacheng City, Tacheng Prefecture
835-1	柳树 Salix sp.	2015/7/11	塔城地区托里县 Tori County, Tacheng Prefecture
839-2	柳树 Salix sp.	2015/7/2	塔城地区塔城市 Tacheng City, Tacheng Prefecture
839-3	柳树 Salix sp.	2015/7/2	塔城地区塔城市 Tacheng City, Tacheng Prefecture
847	柳树 Salix sp.	2015/7/6	塔城地区裕民县 Yumin County, Tacheng Prefecture
856-1	疏锯齿柳 Salix serrulatifolia	2015/7/29	塔城地区沙湾县 Shawan County, Tacheng Prefecture
755-2	红瑞木 Swida alba	2015/6/4	哈密地区哈密市 Hami City, Hami Prefecture
874-2	旱柳 Salix matsudana	2015/7/26	塔城地区沙湾县 Shawan County, Tacheng Prefecture
895-2	柳树 Salix sp.	2015/7/17	塔城地区乌苏市 Wusu City, Tacheng Prefecture
896	灰柳 Salix cinerea	2015/7/14	塔城地区托里县 Tori County, Tacheng Prefecture
897	柳树 Salix sp.	2015/7/14	塔城地区托里县 Tori County, Tacheng Prefecture
901	疏锯齿柳 Salix serrulatifolia	2015/7/14	塔城地区托里县 Tori County, Tacheng Prefecture
919-1	旱柳 Salix matsudana	2015/7/22	塔城地区塔城市 Tacheng City, Tacheng Prefecture
933	金丝垂柳 Salix × aureo-pendula	2015/8/3	塔城地区塔城市 Tacheng City, Tacheng Prefecture
935	金丝垂柳 Salix × aureo-pendula	2015/8/3	塔城地区塔城市 Tacheng City, Tacheng Prefecture
942	旱柳 Salix matsudana	2015/8/4	塔城地区塔城市 Tacheng City, Tacheng Prefecture
944-2	柳树 Salix sp.	2015/8/10	哈密地区哈密市 Hami City, Hami Prefecture
976-1	柳树 Salix sp.	2015/8/10	五家渠市 Wujiaqu City
976-2	柳树 Salix sp.	2015/8/10	五家渠市 Wujiaqu City
863	旱柳 Salix matsudana	2015/7/29	塔城地区沙湾县 Shawan County, Tacheng Prefecture
921	柳树 Salix sp.	2015/7/31	塔城地区塔城市 Tacheng City, Tacheng Prefecture
922	疏锯齿柳 Salix serrulatifolia	2015/7/31	塔城地区塔城市 Tacheng City, Tacheng Prefecture
930-1	疏锯齿柳 Salix serrulatifolia	2015/8/3	塔城地区塔城市 Tacheng City, Tacheng Prefecture

菌株 Isolate	寄主 Host	采集时间 Collecting time	采集地点 Location
319-1	苹果 Malus sp.	2011/8/7	昌吉吉木萨尔县 Jimusar County, Changji Prefecture
790-2	苹果 Malus sp.	2015/6/28	塔城地区塔城市 Tacheng, Tacheng Prefecture
908	苹果 Malus sp.	2015/7/17	塔城地区乌苏市 Wusu, Tacheng Prefecture
936	苹果 Malus sp.	2015/8/4	塔城地区塔城市 Tacheng, Tacheng Prefecture
701	榆树 Ulmus sp.	2015/4/18	吐鲁番市鄯善县 Shanshan County, Turpan City
1155	榆树 Ulmus sp.	2015/8/20	昌吉州奇台县 Qitai County, Changji Prefecture
600-2	胡杨 Populus euphratica	2012/8/20	喀什地区泽普县 Zepu County, Kashgar Prefecture
626	新疆杨 Populus alba	2012/8/20	喀什地区喀什市 Kashgar City, Kashgar Prefecture
686-1	山杨 Populus davidiana	2014/8/31	塔城地区塔城市 Tacheng City, Tacheng Prefecture
686-2	山杨 Populus davidiana	2014/9/1	塔城地区塔城市 Tacheng City, Tacheng Prefecture
702	小叶杨 Populus simonii	2015/4/18	吐鲁番市鄯善县 Shanshan County, Turpan City
737	钻天杨 Populus nigra	2015/6/4	哈密地区哈密市 Hami City, Hami Prefecture
739	钻天杨 Populus nigra	2015/6/4	哈密地区哈密市 Hami City, Hami Prefecture
740	钻天杨 Populus nigra	2015/6/4	哈密地区哈密市 Hami City, Hami Prefecture
742	钻天杨 Populus nigra	2015/6/4	哈密地区哈密市 Hami City, Hami Prefecture
793	俄罗斯杨 Populus russkii	2015/6/29	塔城地区塔城市 Tacheng City, Tacheng Prefecture
882	北京杨 Populus beijingensis	2015/7/22	塔城地区乌苏市 Wusu City, Tacheng Prefecture
891-1	俄罗斯杨 Populus russkii	2015/7/17	塔城地区乌苏市 Wusu City, Tacheng Prefecture
891-3	俄罗斯杨 Populus russkii	2015/7/17	塔城地区乌苏市 Wusu City, Tacheng Prefecture
904	大叶杨 Populus lasiocarpa	2015/7/16	塔城地区乌苏市 Wusu City, Tacheng Prefecture
914-1	杨树 Populus sp.	2015/7/21	塔城地区乌苏市 Wusu City, Tacheng Prefecture
934	新疆杨 Populus alba	2015/8/3	塔城地区乌苏市 Wusu City, Tacheng Prefecture
1074-2	胡杨 Populus euphratica	2015/8/26	吐鲁番市托克逊县 Toxon County, Turpan City
1076	胡杨 Populus euphratica	2015/8/26	吐鲁番市托克逊县 Toxon County, Turpan City
812-1	柳树 Salix sp.	2015/7/1	塔城地区塔城市 Tacheng City, Tacheng Prefecture
835-1	柳树 Salix sp.	2015/7/11	塔城地区托里县 Tori County, Tacheng Prefecture
839-2	柳树 Salix sp.	2015/7/2	塔城地区塔城市 Tacheng City, Tacheng Prefecture
839-3	柳树 Salix sp.	2015/7/2	塔城地区塔城市 Tacheng City, Tacheng Prefecture
847	柳树 Salix sp.	2015/7/6	塔城地区裕民县 Yumin County, Tacheng Prefecture
856-1	疏锯齿柳 Salix serrulatifolia	2015/7/29	塔城地区沙湾县 Shawan County, Tacheng Prefecture
755-2	红瑞木 Swida alba	2015/6/4	哈密地区哈密市 Hami City, Hami Prefecture
874-2	旱柳 Salix matsudana	2015/7/26	塔城地区沙湾县 Shawan County, Tacheng Prefecture
895-2	柳树 Salix sp.	2015/7/17	塔城地区乌苏市 Wusu City, Tacheng Prefecture
896	灰柳 Salix cinerea	2015/7/14	塔城地区托里县 Tori County, Tacheng Prefecture
897	柳树 Salix sp.	2015/7/14	塔城地区托里县 Tori County, Tacheng Prefecture
901	疏锯齿柳 Salix serrulatifolia	2015/7/14	塔城地区托里县 Tori County, Tacheng Prefecture
919-1	旱柳 Salix matsudana	2015/7/22	塔城地区塔城市 Tacheng City, Tacheng Prefecture
933	金丝垂柳 Salix × aureo-pendula	2015/8/3	塔城地区塔城市 Tacheng City, Tacheng Prefecture
935	金丝垂柳 Salix × aureo-pendula	2015/8/3	塔城地区塔城市 Tacheng City, Tacheng Prefecture
942	旱柳 Salix matsudana	2015/8/4	塔城地区塔城市 Tacheng City, Tacheng Prefecture
944-2	柳树 Salix sp.	2015/8/10	哈密地区哈密市 Hami City, Hami Prefecture
976-1	柳树 Salix sp.	2015/8/10	五家渠市 Wujiaqu City
976-2	柳树 Salix sp.	2015/8/10	五家渠市 Wujiaqu City
863	旱柳 Salix matsudana	2015/7/29	塔城地区沙湾县 Shawan County, Tacheng Prefecture
921	柳树 Salix sp.	2015/7/31	塔城地区塔城市 Tacheng City, Tacheng Prefecture
922	疏锯齿柳 Salix serrulatifolia	2015/7/31	塔城地区塔城市 Tacheng City, Tacheng Prefecture
930-1	疏锯齿柳 Salix serrulatifolia	2015/8/3	塔城地区塔城市 Tacheng City, Tacheng Prefecture

编号 No.	引物 Primer	引物序列(5′-3′) Primer sequence (5′-3′)	退火温度 Annealing temp. (℃)
1	IJ-5	GAGAGAGAGAGAGAGATG	52
2	IL-7	ACAACACACACACACAC	52
3	IJ-7	GGGTGGGGTGGGGTG	52
4	IJ-9	AGATGTGTGTGTGTGTG	52
5	IJ-8	ATAAGAGAGAGAGAGAG	52
6	ID-8	GTGCGTGCGTGCGTGC	52
7	IL-3	CACGAGAGAGAGAGAGA	52
8	IQ-2	CCAAACCACCACCACCACCA	53
9	IJ-3	AGAGAGAGAGAGAGAGTC	53
10	IJ-4	GAGAGAGAGAGAGAGATC	53

编号 No.	引物 Primer	引物序列(5′-3′) Primer sequence (5′-3′)	退火温度 Annealing temp. (℃)
1	IJ-5	GAGAGAGAGAGAGAGATG	52
2	IL-7	ACAACACACACACACAC	52
3	IJ-7	GGGTGGGGTGGGGTG	52
4	IJ-9	AGATGTGTGTGTGTGTG	52
5	IJ-8	ATAAGAGAGAGAGAGAG	52
6	ID-8	GTGCGTGCGTGCGTGC	52
7	IL-3	CACGAGAGAGAGAGAGA	52
8	IQ-2	CCAAACCACCACCACCACCA	53
9	IJ-3	AGAGAGAGAGAGAGAGTC	53
10	IJ-4	GAGAGAGAGAGAGAGATC	53

观察项 Item	代表值 Value
观察项 Item	0	1	2	3	4	5
菌落颜色 Colony color	白色 White	米黄色 Light yellow	土黄色 Khaki	灰色 Gray	深灰色 Dark gray	桔红色 Orange
菌落形状 Colony shape	圆形生长 Circular growth	辐射状生长 Radial growth	-	-	-	-
表面形态 Surface morphology	菌丝稠密 Hyphae dense	菌丝稠密 Hyphae dense	菌丝稠密 Hyphae dense	菌丝稠密 Hyphae dense	菌丝稠密 Hyphae dense	菌丝稀疏 Hyphae sparse
	平伏 Flat exhibition	轻微凸起 Slight raised	轻微凸起 Slight raised	凸起 Raised	凸起 Raised	轻微凸起 Slight raised
	无轮纹 No concentric ring	无轮纹 No concentric ring	有轮纹 Concentric ring	无轮纹 No concentric ring	有轮纹 Concentric ring	无轮纹 No concentric ring
有/无子实体 Sporulation	子实体小 Small sporophore	子实体小 Small sporophore	子实体大 Larger sporophore	子实体大 Larger sporophore	-	-
有/无子实体 Sporulation	密集型 Intensive	稀疏型 Sparse	稀疏型 Sparse	聚生型 Concentrated	-	-
色素分泌 Pigment secretion	米白色 Creamy-white	米黄色 Light yellow	土黄色 Khaki	灰色 Gray	黑色 Black	桔红色 Orange