Diversity of IAA-producing endophytic bacteria isolated from the roots of Cymbidium goeringii

doi:10.3724/SP.J.1003.2010.195

Abstract

Abstract:

Endophytic bacteria can directly stimulate plant growth in several ways, including production of plant hormones such as indole-3-acetic acid (IAA). We investigated the diversity of IAA-producing endophytic bacteria in the roots of Cymbidium goeringii using culture-dependent approaches. A total of 57 IAA-producing strains were obtained from 256 strains isolated from the interior roots of C. goeringii. Based on amplified ribosomal DNA restriction analyses (ARDRA) and 16S rDNA sequences, these 57 strains were divided into 25 groups. The IAA-producing endophytic bacteria were grouped into six phyla, i.e., Alphaproteobacteria (35.1%), Firmicutes (33.3%), Gammaproteobacteria (14.0%), Betaproteobacteria (8.8%), Actinobacteria (7.0%) and Bacteroidetes (1.8%). The dominant groups were Alphaproteobacteria and Firmicutes, and the dominant genus, Paenibacillus, produced relatively high levels of IAA. In addition, four potential novel taxonomic units were found. Our results revealed diverse and abundant communities of IAA-producing endophytic bacteria in the roots of C. goeringii and highlight the microbial resources represented by plant growth-promoting bacteria.

Key words: Cymbidium goeringii, plant growth-promoting bacteria, ARDRA, 16S rDNA

Lin Liu, Lei Sun, Ruiying Zhang, Na Yao, Lubin Li. Diversity of IAA-producing endophytic bacteria isolated from the roots of Cymbidium goeringii[J]. Biodiv Sci, 2010, 18(2): 182-187.

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URL: https://www.biodiversity-science.net/EN/10.3724/SP.J.1003.2010.195

https://www.biodiversity-science.net/EN/Y2010/V18/I2/182

Figures/Tables 2

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类群 Group	分型测序菌株Isolate	菌株数 No.of strains	IAA分泌量 IAA production (mg·L^-1· (OD₆₀₀)^-1)	最相近菌种(登录号) Nearest strain (accession no.)	序列相似性Similarity (%)
α-变形菌纲Alphaproteobacteria (35.1%)	R209	7	1.0^#-26.3^#	Rhizobium miluonense (EF061096)	100.0
	R128-2	1	5.8^#	R. huautlense (AF025852)	97.1
	R141	1	3.6^#	R. giardinii (U86344)	97.3
	R224-3	4	10.0^#-43.1^#	Skermanella aerolata (DQ672568)	90.1
	R120	2	23.9*-47.1^#	Devosia insulae (EF012357)	97.8
	R90	2	0.5*-1.7^#	Sphingopyxis macrogoltabida (AB372254)	99.8
	R83-1	1	2.3^#	Altererythrobacter epoxidivorans (DQ304436)	96.3
	R178	1	14.9^#	Mesorhizobium opportunistum (AY601515)	97.5
	R191	1	25.8*	Agrobacterium tumefaciens (EU445236)	100.0
β-变形菌纲Betaproteobacteria (8.8%)	R94	2	0.7^#-5.3^#	Variovorax paradoxus (AJ420329)	99.0
	R199	1	1.9^#	V. soli (DQ432053)	98.4
	R163	1	18.9^#	Burkholderia caribensis (Y17009)	99.1
	R235-1	1	13.1*	Herbaspirillum chlorophenolicum (NR_024804)	99.4
γ-变形菌纲 Gammaproteobacteria (14.0%)	R215-2	7	10.1*-14.6^#	Dyella yeojuensis (DQ181549)	100.0
γ-变形菌纲 Gammaproteobacteria (14.0%)	R192	1	3.3*	Pseudoxanthomonas japonensis (AB008507)	99.7
厚壁菌门 Firmicutes (33.33%)	R138	11	1.1*-114.8^#	Paenibacillus agaridevorans (NR_025490)	98.0
	R172	4	37.6^#-44.0^#	P. contaminans (EF626690)	94.6
	R12-2	2	26.3^#-26.4^#	P. alkaliterrae (AY960748)	96.3
	R59	1	7.7^#	Bacillus aerophilus (AJ831844)	100.0
	R95	1	11.6^#	B. niabensis (DQ176422)	99.8
放线菌门 Actinobacteria (7.0%)	R73-1	1	1.7^#	Nocardioides aquiterrae (AF529063)	98.3
	R100	1	15.3^#	Brachybacterium paraconglomeratum (AJ415377)	100.0
	R152	1	9.6^#	Arthrobacter niigatensis (AB248526)	100.0
	R205	1	1.6^#	Streptomyces albaduncus (AY999757)	98.7
拟杆菌门 Bacteroidetes (1.8%)	R156-1	1	2.1*	Chitinophaga terrae (AB267724)	97.4

类群 Group	分型测序菌株Isolate	菌株数 No.of strains	IAA分泌量 IAA production (mg·L^-1· (OD₆₀₀)^-1)	最相近菌种(登录号) Nearest strain (accession no.)	序列相似性Similarity (%)
α-变形菌纲Alphaproteobacteria (35.1%)	R209	7	1.0^#-26.3^#	Rhizobium miluonense (EF061096)	100.0
	R128-2	1	5.8^#	R. huautlense (AF025852)	97.1
	R141	1	3.6^#	R. giardinii (U86344)	97.3
	R224-3	4	10.0^#-43.1^#	Skermanella aerolata (DQ672568)	90.1
	R120	2	23.9*-47.1^#	Devosia insulae (EF012357)	97.8
	R90	2	0.5*-1.7^#	Sphingopyxis macrogoltabida (AB372254)	99.8
	R83-1	1	2.3^#	Altererythrobacter epoxidivorans (DQ304436)	96.3
	R178	1	14.9^#	Mesorhizobium opportunistum (AY601515)	97.5
	R191	1	25.8*	Agrobacterium tumefaciens (EU445236)	100.0
β-变形菌纲Betaproteobacteria (8.8%)	R94	2	0.7^#-5.3^#	Variovorax paradoxus (AJ420329)	99.0
	R199	1	1.9^#	V. soli (DQ432053)	98.4
	R163	1	18.9^#	Burkholderia caribensis (Y17009)	99.1
	R235-1	1	13.1*	Herbaspirillum chlorophenolicum (NR_024804)	99.4
γ-变形菌纲 Gammaproteobacteria (14.0%)	R215-2	7	10.1*-14.6^#	Dyella yeojuensis (DQ181549)	100.0
γ-变形菌纲 Gammaproteobacteria (14.0%)	R192	1	3.3*	Pseudoxanthomonas japonensis (AB008507)	99.7
厚壁菌门 Firmicutes (33.33%)	R138	11	1.1*-114.8^#	Paenibacillus agaridevorans (NR_025490)	98.0
	R172	4	37.6^#-44.0^#	P. contaminans (EF626690)	94.6
	R12-2	2	26.3^#-26.4^#	P. alkaliterrae (AY960748)	96.3
	R59	1	7.7^#	Bacillus aerophilus (AJ831844)	100.0
	R95	1	11.6^#	B. niabensis (DQ176422)	99.8
放线菌门 Actinobacteria (7.0%)	R73-1	1	1.7^#	Nocardioides aquiterrae (AF529063)	98.3
	R100	1	15.3^#	Brachybacterium paraconglomeratum (AJ415377)	100.0
	R152	1	9.6^#	Arthrobacter niigatensis (AB248526)	100.0
	R205	1	1.6^#	Streptomyces albaduncus (AY999757)	98.7
拟杆菌门 Bacteroidetes (1.8%)	R156-1	1	2.1*	Chitinophaga terrae (AB267724)	97.4